66 -- CUSTOM GLASS MICROARRAYS, FLUORESCENT LABELING KITS AND ARRAY SPECIFIC PRIMERS

Notice Date

August 17, 2001

Contracting Office

Department of Health and Human Services, Center for Disease Control and Prevention, Morgantown -- NIOSH, 1095 Willowdale Road, Morgantown, WV, 26505

ZIP Code

26505

Description

NOTICE OF INTENT TO SOLE SOURCE ? The National Institute for Occupational Safety and Health (NIOSH) facility in Morgantown, WV has a requirement to purchase oliogonucleotide primers, fluorescent labeling kits and printing of custom glass microarrays. The NIOSH/HELD/Toxicology and Molecular Biology Branch?s Musculoskeletal Diseases and Inflammatory Processes Project, which includes muscle injury and vibration models in mice, examines the role that inflammatory mediators play in the injury and repair processes. The government intends to negotiate on a non-competitive basis with Clontech Laboratories, Inc., Palo Alto, CA because they possess a majority of the pre-optimized oligonucleotides required for a custom microarray to be used in this project. Specifications for Atlas Ready to Print Long Oligios are as follows: 80 bp long oligos; 8,327 named genes represented; each oligo has less than 70% homology to any entry in the gene bank; each oligo permits discrimination in multigene families and alternative splice forms of genes; each oligo is quality tested via two enzymatic tests which insure signal intensity will be greater than 10 fold less than the strongest signal, oligo sequence integrity is maintained; the oligos are not amine modified and the oligos come with a two-day training seminar. Clontech is the only known vendor that can supply array specific primers, and materials and reagents for indirect fluorescent labeling of probe, which provides a higher dye incorporation efficiency than other available protocols. The specific items the government intends to purchase from Clontech are as follows: #CS2003, custom atlas glass microarray, 1 EACH; additional microarrays , 190 EACH; 37 Non-Atlas genes, 1 EACH; Optional CDS primer mix, 770 PAIR; and #K1037-1-ATLAS GL. Fluorescentlab, 12 EACH. Organizations who believe they can provide products equal to those stated above may respond within 7 days after publication of this notice. Responses shall be submitted to: Brenda L. Goodwin (MS-1019), Reference: #2001-Q-04122, DHHS, PHS, CDC, NIOSH, ALOSH, 1095 Willowdale Road, Morgantown, WV 26505. Information received would generally be used for the purpose of determining whether to conduct a competitive procurement. This notice is issued for informational purposes only.

Web Link

Visit this URL for the latest information about this (http://www.eps.gov/spg/HHS/CDCP/MNIOSH/2001-Q-04122/listing.html)

Record

Loren Data Corp. 20010821/SPMSC004.HTM (D-229 SN50V3G0)