66 -- NANO-CAPILLARY FLOW LIQUID CHROMATOGRAPHY (LC)

Notice Date

June 21, 2001

Contracting Office

USDA, ARS, Pacific West Area, 800 Buchanan St., Albany, CA 94710

ZIP Code

94710

Solicitation Number

113-91H2-01

Response Due

July 23, 2001

Point of Contact

Clarice E. Dixson 510-559-6027 / 510-559-5638 (FAX)

E-Mail Address

USDA, ARS, Western Regional Research Center, Location (cdixson@pw.usda.gov)

Description

One fully configured nano-capillary flow liquid chromatography (LC) system with PC for instrument control and data acquisition, including RS232 extension card; conversion kit for nano LC (10mm nano flow cell, 15cm nano-column, connection tubing); parking kit for nano LC/nano spray/MS/MS with Harvard syringe pump, upchurch microvalve and actuator, software plug-in; well plate autosampler; Peltier cooling option; column switching unit. Integrated instrument that allows nano flow chromatography and handling of nano liter volumes with ability to operate in a nano flow chromatography mode at 50 to 200 nano liters per minute; specialized hardware/software with specific application in the area of electrospray mass spectrometry. Must meet the following criteria: a capillary HPLC with reciprocating double-piston pump to produce low-pressure proportioned quaternary gradients; capillary HPLC with flow calibrators and flow cells for use as a nano-flow HPLC at 50nl up to 200uL/min; with flexibility to run nano-bore or micro-bore, capillary columns, also capability to run conventional LC columns with minor modification at flow rates of up to 5ml; overall flow rate range of 50 nl/min up to 200 uL/min; gradient delay time of 3 minutes or less, capillary or nano HPLC modes; on-line monitoring of column pressure in capillary and nano-HPLC modes; autosampler with dual needle (fused silica needle for sample pickup, sheathed inside a stainless steel needle) for nL or uL sample pickup from vials, or well plates with zero sample loss, capable of piercing plastic vial caps, and rapid valve switching for low dispersion injection; sample injection volumes down to 20nL; integrated column switching device for in-line sample clean-up, 2-D chromatography, fast injection of large volumes (30uL or more in nano HPLC mode) for gel digest analysis, etc.; UV/Vis scanning detector with flow cell of 10mm path length and 45nL or smaller volume in capillary HPLC mode, 10mm path length and 3nL or smaller volume in nano HPLC mode, capability to monitor 4 wavelength simultaneously; control of the above integrated system from the LCQ software program xCalibur or Analyst mass spec software via a supplied software plug-in; integrated peak parking for increasing sensitivity and the number of mass spectra acquired 20nl per minute; helium sparging of solvents to prevent bubble formation during low flow (peak parking) conditions. System must allow automatic sample introduction into an existing Finnigan LCQ electrospray ion-trap mass spectrometer, provide nano/micro-scale sample analysis, and be capable of performing analysis of proteins present in low copy numbers.

Web Link

none (none)

Record

Loren Data Corp. 20010625/66SOL015.HTM (W-172 SN50P6Q4)