A -- FLUORESCENCE DETECTION OF LIGAND BINDING TO 2-AMINOPURINE LABELED REV

Notice Date

June 19, 2001

Contracting Office

Department of Commerce, National Institute of Standards and Technology (NIST), Acquisition and Logistics Division, 100 Bureau Drive, Building 301, Room B129, Mail Stop 3571, Gaithersburg, MD, 20899-3571

ZIP Code

20899-3571

Description

Researchers at the National Institute of Standards and Technology (NIST) are working on the following technologies: A method is disclosed for selective substitution of highly flourescent nucleotide base analogs within the sequence of nucleic acid drug targets, such that these bases can be used as probes to monitor/screen for the interaction of ligands with a nucleic acid target. In designing the fluorescent nucleic acid target, information about the nucleic acid structure and its native interaction with other macromolecules is used to engineer fluorescent analogs that display fluorescence emission quantum yields that are sensitive to interactions with ligands and/or other macromolecules. The general method of using changes in the fluorescence emission spectra as a probe for the interaction of the nucleic acid target with ligands has been name Flurescence Emission Peturbation (FREP). For further information, contact: National Institute of Standards and Technology, Office of Technology Partnerships, 100 Bureau Drive, Stop 2200, Gaithersburg, MD, 20899; This is not an announcement of a contract action or a grant.

Web Link

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Record

Loren Data Corp. 20010621/SPMSC001.HTM (D-170 SN50P3H4)