B -- DETECTING AUTOANTIBODIES IN SERUM OF CFS PATIENTS

Notice Date

May 22, 2001

Contracting Office

Department of Health and Human Services, Center for Disease = Control and Prevention, Procurement and Grants Office (Atlanta), 2920 = Brandywine Road, Room 3000, Atlanta, GA, 30341-4146

ZIP Code

30341-4146

Solicitation Number

2001-Q-00108

Response Due

June 8, 2001

Point of Contact

Susan Lyle, Purchasing Agent, Phone (770)488-2627, Fax = (770)488-2670, Email SLyle@CDC.GOV -- Susan Lyle, Purchasing Agent, = Phone (770)488-2627, Fax (770)488-2670, Email SLyle@CDC.GOV

Description

The Centers for Disease Control and Prevention (CDC) intends to = procure services of a vendor to determine the presence of = autoantibodies in 200 serum samples, 75 samples from persons with = Chronic Fatigue Syndrome (CFS) and 125 non-fatigue controls. The = vendor shall (a) Measure Rheumatoid Factor in an aliquot of each serum = sample; (b) Identify antibodies to nuclear and cellular autoantigens = that include dsDNA, ssDNA, Sm, U1-RNP, SS-A/Ro, SS-B/La, Scl-70, = Centromere, Histone H3, and Histone H4; (c) Identify antibodies to = microtubule-associated protein 2 (MAP2) and neurofilament triplet (NFT) = proteins using bovine brain and spinal cord extracts. Identify and = localize autoantibodies to neuronal antigens in nerve growth factor = differentiated PC12+ cells by indirect immunofluorescence. The vendor = shall provide the Viral Exanthems and Herpesvirus Branch with the = results of detecting autoantibodies by Elisa, immunohistochemistry and = immunoblot against nuclear cellular and two neuronal antigens. All = tasks are to be completed and a final report shall be provided to CDC = by September 30, 2001. Minimum Vendor Qualifications: (1)Extensive = knowledge and experience in developing and using sensitive and specific = molecular assay for detecting autoantibodies to cellular autoantigens. = (2)Extensive knowledge and experience with characterization of = autoantibodies in chronic disease, rheumatic illnesses, and cancer. = (3)Knowledge and experience in handling and processing limited clinical = material derived from study subjects. (4)Knowledge and experience in = interpreting the public health significance of findings. All inquiries = must reflect the RFQ number. Point of Contact is Sue Lyle at the = address shown, Mailstop K70, or via e-mail at SIL2@cdc.gov, or by = facsimile at 770-488-2670 or 2671. A Request for Quotations will be = issued on approximately June 11, 2001. NAICS 62151 SIC 8071. =09

Web Link

Visit this URL for the latest information about this (http://www.eps.gov/spg/HHS/CDCP/PGOA/2001-Q-00108/listing.html)

Record

Loren Data Corp. 20010524/BSOL002.HTM (D-142 SN50M8H8)