Acquisitions Management Operations Branch, NIAID, 6700-B Rockledge Drive, Room 1130, Bethesda, MD 20892-7605

66 -- SYRINGE QUENCH FLOW SYSTEM SOL NOI-0020 DUE 083000 POC Sophia Janus, Purchasing Agent (301) 594-5223/Rebecca A. Guenthner, Contracting Officer (301) 402-2284 This is a combined synopsis/intent to procure commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation, quotes are being requested, and a written solicitation will not be issued. This procurement is being issued as a notice of intent. Submit offers to NOI # 0020. The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-19. This acquisition is a 100% Small Business Set-Aside. The associated Standards Industrial Classification Code (SIC) for this procurement is 3826. SCHEDULE: The National Institute of Allergy and Infectious Diseases intends to procure a sole source acquisition with the Molecular Kinetics, Inc. The equipment required is a 4-Syringe Quench Flow System, product number SFM-400/Q and Compac Presario XL 106 Notebook Computer. The system includes the following: 4-Syringe Quench Flow Module, user-controlled Variable Ratio Mixing, reagent volume programmable from uL to mL, 2 programmable/sequential aging lines between 3 mixers, 4-syringe/4-motor, Thermostated Module, 4 10-mL Reservoir Syringes of Peek material, 1 MPS-52/4 Microprocessor Control Unit, 1 Driving Sequence Software for Variable Ratio Mixing, 1 Exit valve for quench flow, 4 pairs of delay lines, 17 to 190 uL, sizes up to 1000 uL available. Our laboratory is interested in studying the interactive effects of receptor and RGS proteins on G protein signaling in a variety of the cells (olfactory, B and T cells, neuronal cells, in reconstituted phospholipid vesicles that contain receptor, G-protein or RGS proteins). Most cells respond to extracellular signals via cell surface receptors, G-proteins, and effectors that produce an extracellular messengers (cAMP and IP3). A common property of these signaling systems is that they can adapt to a chronic stimulus, a process known as desensitization. The initiation, amplitute, and terminationof this G-protein signaling are all determined by a tightly controlled cycle of activation and deactivation, known as GTPase cycle. The RGS proteins promote termination reactions by stimulating g-protein GTPase activity. GTP binding and the activation step is promoted by G-protein-coupled receptors, the hydrolysis of bound GTP and consequent deactivation is acceleration by RGS proteins. Each step in the GTPase cycle is very rapid, occuring in a few msec. Because this rapid turn-on and turn-off cycle occurs in msec, it is impossible for us to make these measurements in the normal biochemical assays. Therefore, we need to use a rapid mixing system to study the individual steps in the GTPase cycle. The rapid mixing (stopped-flow) instrument (Bio-Logic SFM4/Q) is available only from Molecular Kinetics (Pullman, WA). This is the only devise available for us to purchase to measure the 2nd messengers (cAMP and IP3) in milli-second time scale. This instrument contains quench-flow mixer in which four-syringe (to mix four different samples), mixing chambers, and delay lines are all under thermostat control. This set up will allow us to mix samples down to 0.8 ms time scale for kinetics experiments such as enzyme kinetics, membrane filtration, and vesicle binding. All of the sample mixing is microprocessor controlled, therefore, the movement of stepping motor driven syringes, the mixing time scale will remain the same throughout the temperature ranges of operation. This instrument can be used in our laboratory using live cells as well as the protein samples for the following purposes: 1. Enzyme kinetics and ion transport studies. 2. To measure the kinetics of the second messenger generation. Since this mixing instrument is built without a pressure overdrive, the live whole cell samples will not be killed and the reconstituted vesicle samples will not be sheared in the mixing process. Delivery location is National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 and shall be inside delivery. FAR provisions and clauses that apply to this acquisition: 52.212-1 Instructions to Offerors Commercial Items; FAR 52.212.-3 Offeror Representations and Certifications Commercial items; FAR 52.212.4 Contract Terms and Conditions -- Commercial items; FAR 52.212-5 Contract Terms and ConditionsRequired to Implement Statutes or Executive Orders Commercial Items. Far 52.225.-3 Buy American Act. North American Free Trade Agreement Israeli Trade Act Balance of Payments Program. Offerors must include with their offer a completed copy of the provisions at FAR 52.212-3 Offerors Representations and Certifications Commercial items. The Government intends to make a Best Value Buy to the responsible Offeror whose offer is the most advantageous to the Government. See Numbered Note 1. Evaluation will include 1.) The technical capability of the item offered to meet the agency need and 2.) Price. Warranty information to include period. Coverage shall be one year from the date of installation. Offeror must include with their offer certification that all hardware and software offered are Y2K compliant. Offers must be submitted not later than 3:00 PM, Eastern Daylight Time (EDT,) August 30, 2000, to Mrs. Sophia S. Janus. Copies of the above referenced clauses are available upon request by telephone from Mrs. Sophia S. Janus at (301) 496-2814. For delivery through the Postal Service the address is Room 1130, NIH/NIAID/AMOB, 6700B Rockledge Dr., MSC 7605, Bethesda, MD 20892-7605. If hand delivered or delivery service the address is Room 1130, NIH/NIAID/AMOB, 6700B Rockledge Dr., MSC 7605, Bethesda, MD 20817-7605. Electronic Submission will not be accepted. Requests for information concerning this requirement are to be addressed to Mrs. Sophia S. Janus at (301) 496-2814. Collect calls will not be accepted. All responsible sources may submit an offer that will be considered by this Agency. Posted 08/03/00 (W-SN481909). (0216)

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