National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, Natcher Building, 45 Center Dr., MSC 6600, Room 6AN32, Bethesda, Maryland 20892-6600

R -- RESEARCH ON DETECTION OF MESSENGER RNA SOL NIH-NIDDK-00-514 DUE 010400 POC Patricia Haun, Purchasing Agent, 301-594-8855 The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) plans to negotiate on a sole source basis with King's College, 26 Drury Lane, London WC2B5RL, England, under the authority of FAR 6.302 for the services of Dr. David Fear. Dr. Fear's technical expertise, particularly during the last year, in research on detection of messenger RNA at low levels in single cells, and particularly cells expressing germline transcripts at the immunoglobulin heavy chain locus cannot be duplicated. He is also an expert in the study of the mechanism of class switching within the human immunoglobulin heavy chain locus, in particular with respect to the role of chromatin structure in these processes. These represent the preliminary and necessary steps in carrying out this project to determine the chromatin structure and patterns of activation of the germ line transcripts in appropriate transformed cell lines and B cells. Dr. Fear is the only one who can technically carry out the above techniques based on the development of his research during the past year. Interested vendors may identify in writing their interest with a capability statement to the Contracting Office prior to the closing date of this notice. This notice of intent is not a request for competitive proposals, however, all responses received before the closing date of this announcement will be considered by the Government. A determination by the Government not to compete this proposed acquisition is based upon responses to this notice and is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. It is anticipated that Request for Quotation (RFQ) NIH-NIDDK-00-514 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due ten (10) days after the release of the RFQ. The anticipated award date is January 4, 2000. Request for copies of the RFQmust be in writing and may be FAXED to 301-480-4226. Quotations must be submitted in writing to the National Institute of Diabetes and Digestive and Kidney Diseases, 6705 Rockledge Drive, Building One, Room 8048, Bethesda, Maryland 20817 Attention: Patricia Haun. FAX quotations must contain offerors name, address, telephone number and fax number. FAX quotations will only be accepted if dated and signed by an authorized company representative. Posted 12/03/99 (W-SN405449). (0337)

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