National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- SYMPHONY MULTIPLEX PEP SYNTHESIZER SOL RFQ-NCI-90231 DUE 092199 POC Cynthia Brown, Purchasing Agent, 301-402-4509, Todd Cole, Contracting Officer The National Cancer Institute (NCI) plans to procure a Symphony Multiplex Peptide Synthesizer,computer, color monitor, printer, 12 glass reaction vessels, solvent cabinent, waste tanks, a start-up kit, a 20L vessel assembly and on site installation and training from Rainin Instrument Company, P.O. Box 4026, Mack Road, Woburn, MA 01801. A multiplex peptide synthesizer is needed to perform several structural studies in one of the NCI laboratories. Since there is a heavy volume of syntheses across different reaction times and protocols, a system that allows for simultaneous production of completely independent compounds and in different quantities is required. This will enable work to be completed in a more timely, flexible, safe and efficient manner. The required system shall perform rapid and efficient solid phase synthesis of large numbers of compounds with different sequencers. The instrument shall allow at least 12 reaction vessels to produce compounds quickly and in ample numbers with many reaction vessels. In addition, the valve system shall be a patented multiplexing matrix style that permits different reagents to be transferred to different reaction vessels (Rvs) without cross-contamination. The instrument shall allow different reaction times and protocols to be applied for each synthesis, allowing the instrument to be used for simultaneous production of completely independent compounds. It shall enable compounds of differing lengths to be synthesized simultaneously in different quantities. The reagents shall be dispensed from common reservoirs for the synthesis of all compounds. The system shall also allow for the use of bulk reagents to be used to prepare solutions for 20 or more reagent reservoirs. The instrument shall also enable the reaction vessels to be agitated during coupling to allow the synthesis of larger compounds and permit the solution to be only moderately concentrated for easy dissolution of reagents. The machine shall also provide automated cleavage to deliver the product from each synthesis directly to a centrifuge tube in a vented compartment in order to be set up for further work up of the material. A variable scale of 0.005 mM to 0.35 mM or greater shall be provided with the equipment to enable the synthesis of both large and small quantities. All vessels and steps for different peptides being processed shall be acted upon simultaneously, as opposed to independently. In addition, if peptides are done at varying times, there shall be no waiting or stoppage of the synthesis to remove completed reaction vessels and to replace them with a blank. In addition, to improve product quality, the couplings shall occur under an inert atmosphere of Nitrogen. In summary, the instrument shall possess the following features: 1) At least 12 reaction vessels; 2) The capability to perform 12 different coupling protocols simultaneously; 3) Liquid sensors; 4) The capability of simultaneous solution transfer via a matrix valve; 5) The ability to perform cleavage via a fully automated and enclosed cleavage system built into the instrument; 6) The capability to measure delivered volumes of reagents via a sample loop; 7) The ability to accomplish syntheses completely independent of one another but simultaneously; 8) Agitation completed through nitrogen; 9) The ability to transfer solutions by nitrogen; 10) The ability to use standard protocols or customized reaction protocols for each individual synthesis; 11) The ability to use a conventional synthesizer; and 12) The capability of being used/compatible with standard laboratory ventilation systems. Rainin Instrument is the only source known to the NCI Researcher that can meet most of the above specifications. This notice of intent is not a request for competitive quotation. However, if any interested party believes it can meet the above requirements, it may submit a statement of capabilities. The capability statement and any other furnished information must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can fully meet the requirements. Capability statements must be received in the contracting office by 3:00 p.m., EST on September 21, 1999. If you have any questions, please contact Cynthia Brown, Purchasing Agent on (301)402-4509. A determination by the Government not to compete this proposed requirement based on responses to this notice is solely within the discretion of the Government. Information received shall be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 09/03/99 (W-SN376369). (0246)

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