National Institute of Environmental Health Sciences, Acquisitions Management Branch, 79 T.W. Alexander Drive, Building 4401 Research Commons, P.O. Box 12874, Research Triangle Park, NC 27709

66 -- RECONSOLING OF EXISTING NMR SPECTROMETERS, POSSIBLE ACQUISITION OF 600 MHZ SPECTROMETER!! POC Contact G.H. Earle, Jr., Contracting Officer (919)541-0377 The NIEHS is interested in obtaining the reconsoling of 500 MHZ NMR spectrometers, the possibility of purchasing a 600 MHZ spectrometers indicated in 8 above, plus other possible options as described below. This is a combined synopsis/solicitation for commercial items, and is prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The Request for Proposal (RFP) number is 273-99-P-0024 and incorporates provisions and clauses through FAC 97-12. The solicitation is not set aside and is under SIC 3826, 500 employees. Delivery must be made within 90 days after award of contract. The NIEHS may purchase one or any combination of the following. Statement of Work; possible options of the reconsole of 500 MHZ spectrometers and/or purchase of a new 600 MHZ spectrometer are as follows: Option 1: Reconsole of Unity 500 NMR spectrometer (no trade-in). Replace a Unity 500 NMR console with a new, complete, state-of-the-art three radio frequency (RF) channel 500 MHZ NMR console which will be interfaced with an 11.75 Tesla, 51 mm room temperature bore, Oxford magnet (cryostat # DLN0300/14/5). The console must be equipped to carry out the latest state-of-the-art multidimensional NMR experiments for protein structure determination, equivalent to those in Varians latest ProteinPack software package, and be up gradable to 8 RF channels and 4 receiver channels. The console must have the same specifications as a new Varian Unity Inova 500 console, or equivalent. The console must be equipped with: 1) a state-of-the-art 1H{13C, 15N} triple resonance probe with z-gradient capability, having resolution, line shape, and sensitivity specifications given below; 2) the equivalent of Varian s 40 channel Ultra nmr shims, capable of achieving the resolution and line shape specifications given below; 3) a complete magnet interface; 4) a variable temperature controller, with temperature range of -150 C to 200 C, settable in 0.1 C steps, with 0.1 C stability. 5) Latest version of NMR software. The three RE channels will consist of one 1H RF channel (frequency range of 499.5-500.5, 0.1 Hz resolution), equipped with a state-of-the-art waveform generator, and one fullband RF channel (frequency range of 12-609.5 MHZ, 0.1 Hz resolution at 12-310 MHZ, 0.2 Hz resolution at 310-609.5 MHZ), equipped with a state-of-the-art waveform generator, and one broadband RF channel (frequency range of 12-309.5 MHZ, 0.1 Hz resolution). The combination 1H{130, 15N} triple resonance probe with z-gradient, RE system, 1H/19F GaAs FET preamplifier/receiver system, and 40 channel Ultra nmr shims, or equivalent must demonstrate the following minimum specifications: Resolution and Line shape 1H(1% CHCl3) spinning non-spinning 50% 0.45 Hz 0.65 Hz .55% 5.0Hz 6.0Hz .11% 1 0.0 Hz 12.0Hz Sidebands 1% Signal/Noise 1H Sensitivity 900/1 (0.1% EB in Wilmad 535-PP tube) Pulse PerformanceChannel 9O0Pulse Width RF Homogeneity 1H 7 s @ 30 W 450 /90 80% 810 /900 70% 13C 15.5 s @250 W 360 /0~ 80% 720 100 70% 15N 39.5 ~is ~ 250 W 3 60 /00 80% 720 /00 70% Gradient Strength 60 Gauss/cm Gradient Recovery < 50 s Variable Temperature Range -200C to 800C Sample Volume 600 l with 5 mm standard tube; 330 l with 5 mm Shigemi tubes Reconsole of Unity 500 NMR spectrometer as above, plus upgrade of existing 4 RF channel Unityplus 500 NMR console to state-of-the-art Varian Unity Inova console, or equivalent. The existing 4 channel Unityp/us 500 console contains 2 highband/lowband AMT amplifiers, 2 fullband RF channels (12-609.5 MHZ), each equipped with waveform generators, utilizing 2 PTS 620 synthesizers, and 2 broadband (12-309.5) RE channels, utilizing 2 PTS 320 synthesizers. The Unityp/us 500 console upgrade must include: 1) replacement of 1H and 19F preamplifier with high sensitivity, low noise GaAs FET preamplifier; 2) upgrade 28 channel Super Shims to 40 channel Ultra nmr shims; 3) upgrade console-to-magnet interface to be consistent with a state-of-the-art Varian Unity nova console. The fully upgraded console must have the capability of achieving the same probe specifications with a state-of-the-art 1H{13C, 15N} triple resonance z-gradient probe as given in Option 1. Option 3: Option 2 plus upgrade of Unityplus 400 to state-of-the-art Varian Unity Inova 400, or equivalent. The existing 2 RF channel Unityplus 400 console contains one highband/lowband AMT amplifier, one 1H RF channel (399.5-400.5 MHZ), and one fullband (1 2-489.5 MHZ), utilizing one PTS 500 synthesizer. Option 4: Purchase of a two RF channel 600 MHZ NMR spectrometer. Instrument should have two RF channels and be capable of modern NMR experiments used for the elucidation of the structure of complex molecules. Spectrometer should be up gradable to 8 RF channels, gradients, etc. System should include one 1H{13C, 15N}triple resonance probe with z-gradient capability, and latest version of appropriate NMR software. Option 5: Option 4 plus trade-in of existing 2 RF channel Unity 500 NMR spectrometer, including 11.75 Tesla, 51 mm room temperature bore, Oxford magnet (cryostat # DLNO300/07/1). The existing 2 channel Unity 500 console contains 1 highband/lowband AMT amplifiers, 2 fullband RF channels (12-500 MHZ), each equipped with waveform generators, utilizing 2 PTS 500 synthesizers. Option 6: Option 4 plus trade-in of existing Unityp/us 500 NMR spectrometer, including 11 .75 Tesla, 51 mm room temperature bore, Oxford magnet (cryostat # DLNO300/38/1), equipped with TMC vibration isolation legs. The existing 4 channel Unityplus 500 console contains 2 highband/l6wband AMT amplifiers, 2 fullband RF channels (12-609.5 MHZ), each equipped with waveform generators, utilizing 2 PTS 620 synthesizers, and 2 broadband (12-309.5) RF channels, utilizing 2 PTS 320 synthesizers. The spectrometer is equipped with Varian s 28 channel Super Shim RT shims. In this case, the 600 MHZ spectrometer must be similarly equipped, including TMC legs, 4 RF channels, z-gradient capability and requires one 1H{13C, 15N} triple resonance probe with z-gradient, with the same specifications given in Option 1, with the exception that the 1H 0.1% EB sensitivity should be 1200/1, with a Wilmad 535-PP tube. Technical Evaluation Criteria The technical evaluation will be based on four criteria- 1) technical experience of the offeror (40 points); 2) system performance as judged by spectra provided by the offeror (40 points); 3) compatibility with existing systems (1 0 points); 4) NM R service and support record of the offeror (10 points), as described below: a. Technical Experience (Technical maturity, and Technical Stability of the Offeror, Product, and Personnel) -- 40 points; The technical maturity and stability of the Contractor, product, and product line proposed will be evaluated to assure that a high level of technical excellence will be given to the purchase, installation, training, and to the service to be provided under the warranty requirement. The offeror should provide information with regard to similar instruments in the field, including current user lists, to demonstrate experience in delivering a product that meets requirements for state-of-the-art NMR spectroscopy as described in this solicitation. b. System Performance -- 40 points; The performance of all spectrometers, upgrades, reconsoles, etc. will be evaluated based on the tests described in: J. B. Vaughn and P. L. Kuhns, "How to Write Specifications, Do Demonstrations and Evaluate NMR Spectrometers: Spectroscopy 10, 36-40; 1995 where applicable (clearly, configurations lacking gradients will not be evaluated for gradient performance, etc.) Sensitivity tests involving state-of-the-art triple resonance probe, GaAs FET preamplifier, and 40 channel shim sets, should reflect an increase in signal-to-noise ratio on the order of 33%, over those specified in the above article, consistent with improvements in NMR technology since 1995 and given ir the technical specifications. In addition, vendor should provide WET and/or WATERGATE NOESY spectra of 1 mM ubiquitin in 90% H20 or equivalent protein, obtained on representative NMR spectrometer. Vendor should provide instrument/console/upgrade specifications plus data obtained following as many of the tests included in the above article as possible. c. Compatibility with existing systems -- 1 0 points; The offeror shall be evaluated on the overall compatibility of the components with the existing Oxford 11.75 T magnet, such that the Government is insured a completely compatible system. In the case of the Unity Plus to INOVA upgrades, compatibility with existing Varian NMR console is required. Compatibility with available Varian software will also be considered. d. Instrument service record -- 10 points; The offeror shall be evaluated on the quality of service provided for instrument repair and maintenance. Factors considered include: location of service personnel in or near the RTP area; data provided by the offeror indicating the satisfaction of other users with the service support for NMR spectrometers. Price will not be evaluated. However, if two or more offers are within seven points of each other, they will be considered technically equal and the lowest priced offer will be receive the award. Offers shall include a completed copy of the certifications at 52.212-3, Offeror Representations and Certifications-Commercial Items (June 1999) with offers. Clause 52.212-4, Contract Terms and Conditions-Commercial Items (May 1999) applies. The following sections of 52.212-5, Contract Terms to Implement Executive Orders-Commercial Items (May 1999) apply 1, 5, 11-13 + 23. The successful offeror shall provide training to selected NIEHS employees. There are no numbered notes that apply. Offers are due by COB 31 August 1999. For information call (919)541-2724.***** Posted 08/09/99 (W-SN365098). (0221)

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