NCI Frederick Cancer Research and Development Center (NCI-FCRDC), P.O. Box B, Frederick, Maryland 21702-1201

A -- SUPERNATANTS FOR LYMPHOMA VACCINE SOL S99-263 DUE 081299 POC Dennis J. Dougherty, Contracting Officer 301-846-1087, Marilyn H. Buchen, Contract Specialist 301-846-1527 E-MAIL: Click here to contact the contract specialist via, mbuchen@mail.ncifcrf.gof. SAIC Frederick, as Operations and Technical Support contractor to the National Cancer Institute (NCI) for the Frederick Cancer Research and Development Center is seeking a subcontractor which, following protocols provided by the National Cancer Institute (NCI), will generate supernatants containing patient specific immunoglobulins (PSI) for use in the production of experimental vaccines for B-cell lymphoma. These supernatants containing PSI will come from hybridoma cell lines generated by the fusion of the patients tumor cells with a mouse-human heterohybridoma cell line. Supernatants from these fusion lines will contain the same PSI as the original tumor cells. The subcontractor will be required to: 1) expand the fusion cell lines, 2) generate supernatants containing PSI, and 3) assay these supernatants for PSI, all under well controlled conditions using either Good Laboratory Practices (GLP) or Good Manufacturing Practices (GMP) as necessary. Supernatants will be generated in small hollow-fiber bioreactors according to a protocol provided by the NCI, and should be shown by the contractor to be sterile, mycoplasma-free, and non-reactive in an in vitro adventitious-virus assay. It is anticipated that the subcontractor will produce approximately 50 of these supernatants containing PSI per year, with each supernatant containing between 100-200 mg of PSI. The subcontractor will not be responsible for the generation of the fusion cell lines or the purification of the PSI. Statements of capability should be sent to the contract specialist at: NCI-FCRDC, SAIC Frederick, P.O. Box B, Frederick, MD 21702-1201; e-mailed to mbuchen@mail.ncifcrf.gov; or faxed to 301-846-5414 by August 12, 1999. Posted 07/13/99 (W-SN353392). (0194)

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