National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, Natcher Building, 45 Center Dr., MSC 6600, Room 6AN32, Bethesda, Maryland 20892-6600

66 -- NEW OR RECONDIITONED ELECTROSPRAY IONIZATION TANDEN MASS SPECTROMETER AND ACCESSORIES SOL NIH-NIDDK-99-469 DUE 071999 POC Desiree Kelly, Purchasing Agent 301-594-7731 The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has requirement to purchase a new or reconditioned Electrospray Ionization Tandem Mass Spectrometer and accessories or equivalent. The instrument must have a mass accuracy of 0.01% over the molecular weight range 30 to 3000 amu. It must be capable of performing a product ion scan with sufficient accuracy that all singly charged fragment ions in the product ion spectrum of the doubly charged molecular ion of renin substrate peptide (m/z=880) will show unit resolution, using infusion of no more than 10 picomole/microliter solution; or the equivalent using another peptide of m/z 569, using a 10 picomole/microliter solution infused in the instrument. The MS/MS transmission should be such that the sum of all fragment ions resulting from the m/z=609 precursor from 1 picomole/microliter infused reserpine at 5 microliters/min will be equal to or greater than the original precursor ion intensity measured under MS/MS conditions but without collision gas. The instrument must have sensitivity to detect 80 femtomoles of reserpine introduced through the ion spray inlet at a flow of 40 microliters/min with a signal/noise ratio of at least 10 when monitoring the m/z 609 ion; and 1 picomole of myoglobin will yield a base peak at least 3 times higher than background. A dynamic range of 4x 106 is required. The system must not be larger than 22x30x48 inches in order to fit into the designated laboratory space. The system must be able to run on line power and all needed transformers must be included. The system must include a moveable bench with enough space for the spectrometer and liquid chromatography system. The system must be equipped with a standard ionization spray source and an ultra-low flow source capable of 20 nanoliters/min. Software for data acquisition and analysis is to be included, with at least one additional license for off-line analysis at no extra charge. An appropriate computer workstation mustbe included with the system with at least a 17" monitor, 64 MB memory, 4 GB disk drive, and 600 dpi printer. The computer will be equipped with all necessary communication ports for system control. Also one year warranty on parts and labor. Interested vendors may identify in writing their interest with a capability statement to the Contracting Office prior to the closing date of this notice. This notice of intent is not a request for competitive proposals, however all responses received before the closing date of this announcement will be considered by the Government. A determination by the Government Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. It is anticipated that Request for Quotation (RFQ) NIH-NIDDK-99-469 will be available fifteen (15) days after the publication date of this synopsis. All responsible sources may submit a quotation which, if timely received, shall be within the Simplified Acquisition Threshold for CommercialPurchases up to $5million. Receipt of quotations will be due ten- (10) days after the release of the RFQ. The anticipated award date is July 30, 1999. Request for copies must be in writing and may be FAX to 301-480-4226. Quotations must be submitted in writing to the National Institute of Diabetes and Digestive and Kidney Diseases Room: 6AN32, 45 Center Drive Drive MSC 6600j Bethesda, MD 20892 Attention: Desiree Kelly FAX quotations must contain offerors signed by an authorized company representative Posted 07/01/99 (W-SN349606). (0182)

Loren Data Corp. http://www.ld.com (SYN# 0391 19990706\66-0020.SOL)