DHHS, PHS, CDC, NIOSH, ALOSH, 1095 Willowdale Rd., Morgantown, WV 26505-2888

66 -- AMENDMENT 003 HIGH PERFORMANCE LIQUID CHROMATOGRAPH SOL RP98-005, Amendment 003 DUE 012898 POC Brenda Goodwin, 304/285-5882/Contracting Officer, Rex S. Wolfe, 304/285-5883 THIS IS A COMBINED SYNOPSIS/SOLICITATION FOR COMMERCIAL ITEMS PREPARED IN ACCORDANCE WITH THE FORMAT IN SUBPART 12.6, AS SUPPLEMENTED WITH ADDITIONAL INFORMATION INCLUDED IN THIS NOTICE. THIS ANNOUNCEMENT CONSTITUTES THE ONLY SOLICITATION; BIDS ARE BEING REQUESTED AND A WRITTEN SOLICITATION WILL NOT BE ISSUED. This solicitation is issued as Request for Proposal No. RP98-005 (Submission No. 152196), Amendment 003. This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 90-46. The specifications provided herein are substituted in lieu of those stated in the notice posted 12/18/97. The successful offeror shall furnish and install at the National Institutes for Occupational Safety and Health (NIOSH), Morgantown, WV one (1) high performance liquid chromatograph (HPLC) system with components Brand Name or Equal to the following items manufactured by GBC Separations, Concord, MA. Contract Line Item (CLIN) #1, Model LC1150 (Part 100-1155), Multisolvent Quaternaray Solvent Delivery System with in-line membrane degasser and system organizer; CLIN #2, Model LC5000 (Part 99-1234-00) Photodiode Array Detector, with Win Solo; CLIN #3, Model LC1250 (Part 100- 1250), Fluorescent Detector; CLIN #4, Model 1650 (Part 99-0269-51), Advanced Auto-sampler with 160 x 2 ML vial tray; CLIN #5, WinChrom v1.3 (Part 99-0000- 00), Windows based Chromatography Data Management System (2-2) with PDA control and display; CLIN #6, Temperature Control Module, 0.01C precision; CLIN #7, Model FC203B (Part 171011DV) Automated Fraction Collector with 3- way valve, to include valve kit collection plate, code 1 racks, pan tube bar and inlet tubing (manufactured by Gilson, Middleton, WI). SPECIFICATIONS: (Due to possible printing errors of symbols contained in the specification below, a printed copy will be provided upon request) #1-HPLC Specifications Quaternary Gradient Pump -- Hydraulic System: Dual serial spindle driven, self- centering floating variable stroke piston designwith piston displacement from 16- 100 l., Microprocessor controlled stepper motor and synchronous solvent mixing valve assembly, Flow Rate Range: 0-9.99 ml/min in 0.01 ml/min increments, Flow Rate Precision: 0.1% independent of system pressure, Solvent Selection: Up to four solvents may be selected and mixed, Composition Range: 0-100% in 0.1 increments, Composition Accuracy: 0.5% independent of system pressure, Composition Precision: 0.1% RSD binary gradient water/acetonitrile, Delay Volume/Dwell Time: 600-900 ul dependent on system pressure; **Complete, accurate gradient mixture with each piston stroke; **Pressure can be zeroed from the pump's front panel; Compressibility Compensation: User programmable factors compensate for changes in flow rate at varying system pressures and solvent compositions for all commonly used HPLC solvents, Pressure Pulsations: Typically less than 1.0% of system pressure at all pressures greater than 10 Mpa; #2 Photodiode Array Detector: Wavelength Range 190-700 nm, **Noise 1.5 x10 -5 AU @ 190-370 nm, Drift < 1 x 10 -4 AU/hour, Wavelength Accuracy 1.0 nm, Spectral Resolution 0.37 nm (nm/diode) @ 190-370 nm, 1.25 nm (observed) 1.09 nm (nm/diode) @ 190- 700 nm, 3.00 nm (observed), Analysis SoloNet Analysis software for installation on host data system including: Real time spectral display, Chromatogram plots and overlays, Spectral Plots and Overlays, Contour plots, Peak Purity Analysis and Spectral Library and Search; #3 Programmable Scanning Fluorescence Detector: Optical Design: Dual monochromators using concave holographic diffraction gratings, Sensitivity: S/N > 3000 for 4.1 g/l anthracene in MeOH (Excitation 248 nm, Emission 396 nm in methanol, S/N = 3), Lamp: Pulsed xenon with selectable flash rate of 20 or 100 Hz, user-selectable, Detector: UV- enhanced photomultiplier (PMT), 200-650 nm, Flowcell: Hi-purity quartz, Teflon, and PEEK 12 l, 8 l illuminated volume, Maximum pressure 200 psi (14 bar), Fluorescence Range: 0.01-500 FU FS, Standard Operating Modes: Fluorescence, Phosphorescence and Chemiluminescence; #4 Autosampler: Autosampler Design: High precision X-Y-Z sampling probe, flow through' needle fluid path, polymeric SHP seal and electrical actuated Rheodyne valve Model 7010, **Autosampler can be removed from the flow path for higher fidelity with low flow, gradients; Minimum Sample: 3 l (with recommended tapered vial inserts), Programmable Parameters: Non-sequential standard / sample injections, Injection volume, Pre- column derivatization, Priority sample sequence, Replicate injections, Run time, Needle height, Metering syringe speed for sample pickup, and Method Chain Display: 2 x 20 character backlit liquid crystal display, Control: Stand alone through instrument keypad; Computer control with WinChrom Data Management software, External Needle Wash (optional), Needle wash station with built-in peristaltic pump; #5 Chromatography Data Management System (System Control and Data Acquisition) **Single point control of all components and data acquisition/control parameters, **All communications and control must be digital via IEEE or RS232C; No. of HPLC Systems: Up to four systems (1 system/2 channels as standard), with complete control of two fully integrated GBC LC systems and contact relay control of others, No. of Signal Channels: Up to four channels per system. Instrument Control: LC1120/1150 pump and LC1610/1650 autosampler (via IEEE communication). 1: LC1205/1210/1250/1206 UV-Vis detector (Optional). 2: Analog pump control (voltage) (Optional) 3: Batch Analysis: Up to 750 entries indicating vial number, injection volume, method, file name, pre-treatment file and calibration level. Automatic sample collection, data acquisition and result integration. Start Run Trigger: Manual, remote contact closure or opening (1 per instrument). Programmable External Events: 8 per A/D card (solid state relay, capacity up to +24 V, 40 mA). No. of external events = 50. Analog to Digital Conversion: Resolution: 20 bits at all sampling rates. Dynamic range: > 1,000,000:1. Sampling Rate: User selectable, 0.05 60 Hz per channel @ 60 Hz power line frequency (50 Hz max @ 50 Hz power line frequency). Analog Signal Voltage Ranges: -0.5 to + 1.1 Volt or -5.0 to + 11.0 Volt. Data Points Per Run: No limit, storage to hard disk (4 bytes per data point). Data Storage Safety: Stored to disk every 10 seconds. Data saved even in power loss. (** These points cannot be compromised.) Delivery is desired no later than 30 days after receipt of contract. EVALUATION FACTORS: Offers will be evaluated on their ability to meet or exceed the stated requirements and demonstrated past performance on contracts of similar size and complexity to this requirement (include names and addresses of Company/ Government Agency, name and telephone numbers of customer contacts along with contract number, and description of work performed with your offer.) Technical proposals and cost or price are approximately equal in importance. The Government intends to make a single award to the responsible offeror whose proposal conforms to all requirements of the solicitation and is considered the best value to the Government. Offers shall be proposed based on F.O.B. Destination (Morgantown, West Virginia) and the requirements at FAR 52.214.21 "Descriptive Literature" apply. The FAR provisions and address for receipt of offers remain as stated in the original CBD notice posted 12/18/97. Offerors shall ensure that the required forms, price breakdown of components, descriptive literature and a delivery schedule are provided with their offer. This AMENDMENT 003 MUST BE ACKNOWLEDGED with your offer. Facsimile offers are not authorized. Offers that fail to furnish the required information, or reject the terms and conditions of the solicitation may be excluded from consideration. Information regarding this notice can be obtained from Brenda Goodwin at (304)285-5882 or by fax at (304) 285-6083. All responsible sources may submit a response, which shall be considered by the Agency. (0016)

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