NIH,National, Lung, and Blood Institute, COB Procurement Section,, 6701 Rockledge Drive MSC 7902 Bethesda, MD 20892-7902

65 -- MCS ISOTHERMAL TITRATION CALORIMETRY SYSTEM SOL NHLBI-PS-SP-97-146 DUE 080597 POC Deborah Coulter, Purchasing Agent, (301) 435-0368, Fax number (301) 480-3345 The National Heart, Lung, and Blood Institute intends to negotiate with MicroCal, Incorporated, to purchase (1) one MSC Isothermal Titration Calorimetry System; manufactured by Micro Cal, Inc., the specifications for the System are as follows: 1. Isothermal calorimetry heat pulses as small as 0.1 ucal can be detected. Multiple 0.5 ucal isothermal calorimetry heat pulses can be repeated with a standard deviation of 0.05 ucal, while stirring at 300 rpm. 2. Power feed back circuitry measures heat direct ly. 3. Instrument response half-time of sec. For heat perturbation on isothermal calorimetry, utilizing no mathematical "corrections". 4. After entering samples into cells and injection syringe, no further operator attention is required until a calorimetry experiment is completed. 5. Stirring rates for isothermal calorimetry up to 1500 rpm. 6. Modem capability for remote operation, data viewing and data analysis. 7. True windows software for data collection and data analysis. 8. Provides curve-fitting for both independent and interacting binding sites. 9. Curve-fitting routines based on analysis of differential calorimetry data, as contrasted with statistically invalid treatment of integral data. 10. Fixed-in-place 1.3 ml twin cells for both isothermal calorimetry and differential scanning calorimetry, which cells cannot be removed or damaged by operator. 11. Software-controlled isothermal calorimetry upscans to new working temperature, with baseline equilibration typically within 15 min at new temper ature. 12. Automatic software-controlled coupling of isothermal calorimeter injector stepping motor to syringe, using photo-eye system. To include the following components: Ultrasensitive MCS-ITC MicroCalorimeter, 200 MHZ Pentium Computer Tower System with 32MB RAM, 3.2 GB hard drive, 256k Pipelined Burst SRAM Cache, 4STB Virge 4MB, 3D Video Card, a 3.5" floppy drive, CD-ROM drive, keyboard & mouse, Origin tm, MCS Observer, Microsoft Windows tm, 3.11, DOS< Microsoft Office Professional tm, software, Hewle tt Packard LaserJet 5L printer, Neslab RTE-100-D Refrigerated Circulating Bath rate to -10 degree C, Two built-in nanovolt amplifiers, Degassing station, filling cleaning assembly, three injection syringes, Start-up accessories and operating manuals, On-site installation and instruction. The Standard Industrial Classification (SIC) Code 5047, Size Standard 100 employees will be used for this requirement. This notice of intent is not a request for competitive proposals. Interested parties may identify thei r interest and capabilities in response to this requirement. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurement. The purchase order will be awarded fifteen (15) calendar days after the publication date of this notice. All responsible sources may submit a quotati on, which if timely received, shall be considered by the agency. Responses should be submitted to the National Heart, Lung, and Blood, Institute, Contracts Operations Branch, Procurement Section, Rockledge Building 2, Room 6143, 6701 Rockledge Drive, Bethesda, MD 20817-7902, Attention: Ms. Deborah Coulter. Fax quotations will only be accepted if dated and signed by an authorized representative. (0198)

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