Contracts Management Branch, National Institute of Neurological Disorders and Stroke, NIH, Federal Building, Room 901, 7550 Wisconsin Avenue, MSC 9190, Bethesda, Maryland 20892-9190

A -- LARGE SCALE AUTOMATED DNA SEQUENCING OF HUMAN GENES INVOLVED IN NEUROLOGICAL DISORDERS SOL RFP No. NIH-NINDS-96-13 DUE 111596 POC Contracting Officer, Kirkland L. Davis, 301/496-9203 The National Institute of Neurological Disorders and Stroke (NINDS), National Institutes of Health (NIH), has a requirement for the continued development of highly reproducible rapid DNA template production and purification procedures which provide DNA of sufficient purity for highly accurate automated DNA sequence analysis and significantly improve techniques currently used for genotyping and positional cloning. Additional requirements include screening and amplification of clones from lambda, cosmid, or YAC libraries and biological samples. Libraries of plasmid subclones are to be made from the larger clones. Genetic typing with highly informative PCR based markers shall also be required. Work under the proposed contract will support the mission objectives of the NINDS Core DNA Facility. The NINDS Facility provides rapid DNA sequencing and other types of DNA analysis to NINDS scientists for use in Basic Neuroscience and Clinical Neurogenetics projects. It is estimated that the Contractor will be required to provide approximately 5,000 purified DNA template preparations/year by generating nested deletions in clones of interest; perform genotyping in the amount of approximately 300 markers per 200 DNA samples/year; and perform screening of approximately 10 libraries. The sources of DNA shall be provided by the Government. The NINDS DNA Facility will be responsible for evaluation of materials and results. The Contractor will be required to have staff with expertise and experience in molecular biology, nucleic acid biochemistry, computer analysis and robotics; and operational experience in large scale DNA isolation and analysis techniques, basic microbiological techniques, use of DNA modifying enzymes, bacterial transformation, DNA hybridization and use of polymerase chain reaction (PCR). This requirement represents a recompetition or work currently being performed under NINDS contract No. NO1-NS-4-2305, with Genome Therapeutics Corporation, Waltham, MA. The Government anticipates awarding one contract with a performance period of five years. This is not a Request for Proposals. Request for Proposals (RFP) No. NIH-NINDS-96-13 will be issued on or about September 16, 1996, with a tentative closing date for receipt of proposals of November 15, 1996. To receive a copy of the RFP, submit a written request or FAX request to 301/402-4225. Requests must cite the RFP number referenced above. Please supply this office with two (2) self-addressed mailing labels. All responsible sources are encouraged to submit a proposal which shall be considered by the Government. Refer to numbered note 26. (0242)

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