Centers for Disease Control and Prevention (CDC), Procurement & Grants Office, 255 East Paces Ferry Road, N.E., Room No. 419 Atlanta, Georgia 30305

66 -- MATRIX-ASSISTED LASER-DESORPTION TIME-OF-FLIGHT (MALDI-TOF) MASS SPECTROMETER SOL 96-100(N) DUE 060496 POC Contact Grace White, Contract Specialist, at (404)842-6553 This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The solicitation number is 96-100(N) and is issued as a Request for Proposal (RFP). The solicitation and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 90-37. The standard industrial code (SIC) is 3829; the small business size standard is 500 employees. Line Item 001: matrix-assisted laser-desorption time-of-flight (MALDI-TOF) mass spectrometer. Optional Line Item 002: Nd:Yag laser with crystals and lenses suitable for operation at 266 and 355 nm. Optional Line Item 003: Nd:Yag laser with crystals and lenses suitable for operation at 266, 355, and 10,600 nm. Alternate Line Item 004: System as described in Line item 001 except utilizing a Government-furnished Sun SPARC 10/30 computer. I. DESIGN SPECIFICATIONS. A. The analyzer/detector components shall have: (1) an effective linear drift length of at least 1 meter; (2) a high-transmission, gridless, electrostatic ion reflector with independent linear and reflected detectors; (3) the ability to operate in linear (with up to at least 25kV accelerating voltage) and reflector modes, with positive and negative ion detection; (4) an integrated high-speed digitizer (at least 1 GHz) with real-time display update on host computer; (5) hardware/software to permit scanning of post-source-decay products; (6) hardware/software for delayed ion extraction, with operator-selectable delay time and pulse amplitude; (7) hardware/software for collision-induced dissociation at operator-determined collision gas pressures; (8) hardware/software to support selective gating for ions entering the analyzer, so as to permit MS/MS mode of operation. B. The laser system shall: (1) comprise a pulsed (4 nanoseconds or less) nitrogen laser (337 nm); (2) permit operator control (via computer) for continuously variable laser power levels; (3) be designed to permit use of alternative lasers (see optional line items 002 and 003). C. The vacuum system shall employ: (1) dual turbo molecular pumps (operating to at least 10-7 Torr) for the analyzer region; (2) a separate vacuum pump for the source/sample region. D. An automated sample introduction system which accommodates: (1) at least 25 discrete samples; (2) arbitrary access to any x,y position on a continuous target to allow analysis of membrane-blotted samples; (3) a televisual system for observing the sample target; (4) an automated vacuum interlock for sample introduction. E. Data System Hardware/Software shall: (1) include a built-in computer system with real-time operating system to interface with the high-speed detector and control elements in the instrument; (2) include, as operator interface, a second computer based on a POSIX-compliant operating system (NOTE: Line Item 002 contemplates use of a Government-furnished computer as operator interface, with the vendor providing and loading the necessary software only); (3) provide at least 32 MB RAM, 1 GB disk drive, 17'' color monitor, and an ethernet interface (available for connection to an external network) on the computer with which the operator interfaces; (4) provide a user-friendly interface to all the control functions of the spectrometer; (5) update data in real-time as they are captured by the built-in computer; (6) provide a user-friendly, windowed software package for data analysis and manipulation; (7) allow data analysis/manipulation from remote, networked terminals. II. PERFORMANCE SPECIFICATIONS. This instrument is intended primarily for the analysis of peptides, proteins and nucleic acids. Using samples representative of these classes of compounds, demonstrate that an offered instrument meets or exceeds the following performance criteria. Supporting data must be presented in a format that allows independent evaluation. A. Using protein or peptide samples demonstrate that the instrument achieves: (1) a mass range ) 300 kDa; (2) a mass accuracy of +/- 0.05% or better in linear mode with internal calibration and +/- 0.2% or better with external calibration; (3) a mass accuracy of +/- 0.01% or better in reflector mode with internal calibration and +/- 0.05% with external calibration; (4) stability of external calibration over a time period of several hours; (5) a resolution of 12,000 for multiple samples in the low-mass range (e.g. 2000 - 6000 m/z) (that is, near-baseline resolution of isotope mixture); (6) a resolution of 80 in the high-mass range (e.g. ) 65,000 m/z); (7) amplitude data collected from the same sample in linear and reflectron modes, showing an efficiency of reflectron transmission of at least 85%; (8) a gating (see specification I.A.8) resolution of 125; for example, when set to pass 1000 m/z the gate rejects masses outside of the range 996 to 1004; (9) a resolution for daughter ions comparable to that achieved with a parent ion to which the spectrometer is optimally tuned. B. Using oligonucleotide samples demonstrate that the instrument achieves: (1) isotopic resolution in a low-mass range (e.g. 10- to 20-mer); (2) a resolution of 1,200 in a high-mass range (e.g. 25- to 60-mer). The contractor shall provide full warranty coverage for the instrument for a one year period after installation. The contractor shall provide on-site training in operation of the equipment following installation. Training shall be a minimum of three (3) days for four (4) people. Delivery shall be made FOB destination within 150 days of contract award. Delivery and installation shall be made to 1600 Clifton Road, Building 5, Sub-Basement Room 11, Atlanta, GA 30333. Acceptance shall be at 1600 Clifton Road, upon completion of installation, demonstration and training. Payment terms are 80%upon completion on installation and demonstration of compliance with specifications, and 20% upon completion of training. The provision at FAR 52.212-1, Instructions to Offerors--Commercial Items is applicable without addenda. The provision at FAR 52.212-2, Evaluation--Commercial Items is applicable. Technical merit and past performance, when combined, are significantly more important that cost or price. Evaluation of technical merit will include whether the offeror has demonstrated compliance with the stated requirements as well as flexibility of design, over-engineering with a view to support future enhancements and demonstration of quality of analytical results. Offerors must include a completed copy of the provision at FAR 52.212-3, Offeror Representations and Certifications--Commercial Items with all offers. The clause at FAR 52.212-4,Contract Terms and Conditions--Commercial Items is applicable. The following additional terms and conditions are included by addenda: FAR 52.214-34, Submission of Offers in the English Language (APR 1991); FAR 52.214-35, Submission of Offers in U.S. Currency (APR 1991); FAR 52.225-10, Duty-Free Entry (APR 1984); and FAR 52.229-6, Taxes--Foreign Fixed-Price Contracts (JAN 1991). The clause at FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders--Commercial Items applies to this action. The following FAR clauses cited within FAR 52.212-5 are applicable: FAR 52.203-6, Restrictions on Subcontractor Sales to the Government, with Alternate I; FAR 52.203-10, Price or Fee Adjustment for Illegal or Improper Activity; FAR 52.219-8, Utilization of Small Business Concerns and Small Disadvantaged Business Concerns; FAR 52.222-26, Equal Opportunity; FAR 52.222-35, Affirmative Action for Special Disabled and Vietnam Era Veterans; FAR 52.222-36, Affirmative Action for Handicapped Workers; FAR 52.222-37, Employment Reports on Special Disabled Veterans and Veterans of the Vietnam Era; FAR 52.225-9, Buy American Act--Trade Agreements Act--Balance of Payments Program. The Defense Priorities and Allocations System (DPAS) does not apply. Proposals are due at 2:00 PM, June 04, 1996 to the following address: Centers for Disease Control & Prevention, Procurement & Grants Office, Contracts & Purchases Branch, 255 East Paces Ferry Rd., N.E., Room 419, Atlanta, GA 30305. For information regarding this solicitation, contact Grace White at (404) 842-6751. All responsible sources may submit an offer. No telephonic requests for the solicitation package will be accepted. See Numbered Note 12. (0122)

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