National Cancer Institute, Research Contracts Branch, Cancer Etiology Contracts Section, Executive Plaza South, Rm. 620, 6120 Executive Blvd. MSC 7224, Bethesda, Maryland 20892-7224

A -- LABORATORY SUPPORT FOR PROCESSING AND STORAGE OF BIOLOGICAL SPECIMENS FROM PERSONS AT HIGH RISK FROM CANCER SOL RFP N01-CP-61001-21 DUE 061295 POC Barbara A. Shadrick, Contracting Officer, 301/496-8611. The Epidemiology and Biostatistics Program (EBP), National Cancer Institute (NCI) is seeking a Contractor to provide for the maintenance of the existing EBP inventory of biologic specimens and to receive, process and store new samples as they are collected. This is a 100% Small Business Set-Aside, Standard Industrial Classification (SIC) Code 8731 with a size standard of 500 employees. The Contractor shall provide the services described below, in accordance with Contractor-developed, Government-approved protocols: 1) separation and viable cryopreservation of blood mononuclear lymphocytes, 2) separation, aliquotting and storage of serum, plasma and/or urine as needed, 3) cryopreservation of bone marrow samples, 4) storage of tumor extracts, 5) cryopreservation of whole tumor tissue, 6) cryopreservation of intact red blood cells, 7) viable lines, 8) storage of DNA and other biological materials as specified by the NCI Project Officer, 9) extracting of DNA from biologic materials, 10) logging in, labelling and tracking of each vial of each sample employing an NCI developed computerized specimen tracking system, including all laboratory safeguards to insure the fidelity and purity of each sample, 11) maintenance of the previously-established repository currently containing 1 million biological specimens, and 12) allowance for an estimated increase of up to 25% of freezer storage space. The Contractor shall, for example, provide messenger service for pick-up of specimens or inter-laboratory communication from medical care facilities in the Washington, D.C., area or at area transportation centers (i.e., Dulles, D.C. National and BWI Airports), be responsible for recording and monitoring shipping and receiving of specimens to minimize delay or loss, maintain a repository of biologic specimens which shall include frozen serum, plasma, urine, tumor tissue, tumor tissue extracts, whole red blood cells, separated and frozen white blood cells or fractions of white blood cell populations, bone marrow cells, body fluids, lymphoblastoid cell lines, DNA, stool specimens or smears on slides and other types of specimens as specified by the NCI, provide and train primary and backup staff in the operation of a computerized record system for specimens which has been developed and furnished by the NCI, prepare a variety of specimens for storage, utilize freezers equipped with a stylus recording system indicating consistency of temperature, maintain a central alarm indicating consistency of temperature, maintain a central alarm system monitored 24-hours a day, 365 days a year, keep clear records of all manipulations on all specimens and carefully document specimen type, volume, cell concentration, source, ''crisis event'', etc. for each sample, prepare specimens for shipment, supply shipping containers and make arrangements to send biologic specimens to collaborating investigators in an expeditious (e.g. overnight or same day) fashion, inventory, store and maintain a large repository of sera and cells used for immunogenetic tissue typing, be prepared to process up to 1100 mls of blood per day, four days per week, from lymphocyte harvesting (coded from as many as 60 donors per day), handle international shipments of biological specimens (blood components, urine, gastric juice and biopsy specimen) and clearance of these specimens through U.S. and foreign customs, and submit monthly computerized and written reports, annual reports and a final report. Contract will be a cost- reimbursement type for a 60-month period. The total estimated level-of-effort to be provided is 93,100 direct productive labor hours. Award is anticipated by March 1, 1996. The Contractor is expected to provide the facilities and all major equipment. Additional Government-furnished equipment, currently in use under the existing contract (N01-CP-33060 with BTRL Contracts and Services, Inc., dba Biotech Research Laboratory) will be provided. It is anticipated that the RFP will be available on/about May 11, 1995, with responses due within 30 calendar days after the actual date of issuance of the solicitation. All responsible sources may submit a proposal which shall be considered by the NCI. No collect calls will be accepted. See Note(s): 1. (0103)

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