DHHS/PHS/FDA/NCTR, Office of Contracts and Grants Management, NCTR Contracts Branch, Jefferson, Arkansas 72079-9502

66 -- DNA EXTRACTOR POC Contact Contract Specialist Patricia Meyer, 501/543- 7435/Contracting Officer Vicky L. Culp 501/543-7459 The National Center for Toxicological Research (NCTR) has a requirement for a fully automated instrument chemistry system designed specifically for the isolation/extraction of genomic DNA from animal cells. The specifications for the DNA extractor include the following requirements: extract genomic DNA from animal organs, blood, cells or nuclei that have been predigested with proteinase, detergent and chelating agent; have an extraction volume range from 0.5 milliliter to 1.5 milliliter; perform digestion of cells or nuclei prepared from animal organs and blood and then automatically proceed to extraction of DNA; extract DNA from at least 20 samples at one time with no human intervention; extract at least 40 samples at one time with no more than 30 minutes of human intervention; each sample kept separate with no contamination between samples or between runs; extract DNA from at least 40 predigested samples in one working day (8 hours); capable of processing, at one time, samples that each consist of cells, nuclei or predigestions from at least 100 milligrams of tissue; extract DNA from samples that each contain as little as 5 micrograms of DNA and as much as 100 micrograms of DNA; recover at least 70% of the DNA in the sample; product DNA with an A260/A280 absorbance ratio of between 1.7 and 2.0; produce DNA of high quality suitable for a variety of molecular biology applications, i.e., polymerase chain reaction (PCR), restriction digestion, southern blotting and determination of 5-methyldeoxycytidine content by high pressure liquid chromatography; provide DNA at cycle end either as a precipitate or as a solution in low salt aqueous buffer; include operation software, user manual, in- lab installation and in-lab user training. The standard classification (SIC) code is 5049. The only known source for this requirement is Integrated Separation Systems, Natick, MA., 01760. Other firms having the ability to provide the above system are requested to give written notification (including business size classification and the name and telephone number of a point of contact) to the acquisition office listed in this notice with reference to Requisition Number 332027 within 30 calendar days from the date of this publication. Responses should include technical descriptive literature sufficient to determine the firms ability to satisfy the Governments requirement. (0095)

Loren Data Corp. http://www.ld.com (SYN# 0361 19950406\66-0025.SOL)