SOLICITATION NOTICE
66 -- Acquisition of an Applied Biosystems QuantStudio 3 Real-Time PCR System
- Notice Date
- 7/9/2026 7:50:48 AM
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH OLAO BETHESDA MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- 75N98026Q00714
- Response Due
- 7/20/2026 6:00:00 AM
- Archive Date
- 07/21/2026
- Point of Contact
- John Burton
- E-Mail Address
-
burtonjo@mail.nih.gov
(burtonjo@mail.nih.gov)
- Small Business Set-Aside
- NONE No Set aside used
- Description
- Requirements: To ensure continuity of research, NIAMS proposes the acquisition of an Applied Biosystems QuantStudio 3 Real-Time PCR System. qPCR is a core technology used for nucleic acid amplification, quantification, and analysis and supports a wide range of research applications, including gene expression studies, pathogen detection, transcript quantification, and validation of experimental results. The QuantStudio 3 is the closest replacement to the current StepOnePlus systems, allowing researchers to maintain established workflows and consistency in experimental design and data interpretation. The QuantStudio 3 offers several operational advantages, including user-friendly software, compatibility with both Mac and PC platforms, touchscreen operation, and the ability to function as a standalone instrument or in conjunction with a computer. Its thermal block design is similar to the existing systems, minimizing the learning curve for users and supporting a broad range of genomic applications. In addition, the manufacturer offers trade-in credit for the current instruments, reducing the overall acquisition cost while providing access to a fully supported platform with available parts and technical support. Acquiring the QuantStudio 3 at this time will help prevent interruptions to research activities, maintain reliable and consistent data generation, and ensure that NIAMS laboratories have access to a supported and dependable qPCR platform. 4.1 PRODUCT FEATURES/SALIENT CHARACTERISTICS The contractor shall provide one Applied Biosystems QuantStudio 3 Real-Time PCR System or an equal system that meets all the following minimum requirements. 4.1.1. System Features/Benefits The system shall: � Utilize a platform providing 1 TB or storage space for data sharing at user discretion � be used with online scientific apps � come with desktop-based software, allowing single-plate run setup and analysis. 4.1.2. Technical specifications � Instrument The instrument shall: � have a 96 well 0.1 ml plate block format and the option to run fast protocols <30 minutes � not require a field service engineer for set up by end user. Set up should be easy for end user � directly out of the box. � not require a computer for installation � use an RNase P verification plate to verify performance � not require dye calibration upon installation � have dimensions fo 27 x 50 x 40 cm and be designed to maximize horizonal and vertical bench space � be capable of analyzing 96 samples simultaneously in various formats incluing both 96-well plates, 8-tube strips and individual tubes � the 96-well block should be made of at least 3 separated Peltier elements � VeriFlex Blocks. The maximum temperature difference programmable across the block should be 10 degrees C. 5 degrees C should be the maximum difference in temperature allowed between adjacent blocks. � Support reaction volumes of 10-30 uL on 0.1mL the 96-well block � Be factory calibrated with FAM, SYBR Green 1, VIC, NED, ABY, JUN, TAMRA, and ROX dyes � Have melt or dissociation curves that take place in steps with deltaT varying between 0.015 degrees C and 3.66 degrees C � Have a maximum block ramp rate of 9.0 degrees C/sec for the 0.1 mL block � Have a block with a temperature range of 4-99.9 degrees celcius � Have a heated cover with temperature range of 50-110 degreesC 2 � Have a long-lasting bright white LED source with median lifetime of at least 60,000 hours � Have a CMOS imager for data acquisition and collects data for each filter combination in less than 2 seconds � be used with 120-240 V outlets without modification or special plug requirements � Include a heated lid assembly to heat tops of sample plates and minimize reaction evaporation. 4.1.3 Technical specifications � Instrument onboard computer The system shall: � Possess the capability to run in stand-alone mode, without an attached computer � Include a touchscreen interface and store protocols for quick starts with no computer needed � Have a touchscreen interface that enables viewing amplification plots and filtering them by sample, target and task � Have a touchscreen interface that facilitates uploading of protocols or downloading of run files via a USB, local network and the Connect platform � Have a touchscreen interface that allows uploading of protocols and run files directly to the Connect platform � Contain onboard storage with memory capacity of 10 GB for storing 1,000-5,000 standard absolute quantification run files, and be designed for run protection in case of power outages � Be able to be customized through the touchscreen for time zones and service/calibration notification settings � Have a touchscreen interface that supports the creation of local user accounts with PIN-protected access that can be linked to the Connect platform to online accounts on thermofisher.com � Have a touchscreen interface that allows creation and running of protocols as guests � Have a touchscreen interface that allows for editing of protocols at all stages of the reaction cycle as well as block settings, temperature settings duration, ramp rate, etc. � Have a touchscreen interface that allows a template to be loaded from a USB, local network or the Connect platform � Have a touchscreen interface that allows user to enter reagent information � Have a touchscreen interface that allows a run to be paused and during that pause for the block temperature to be set as well as for the drawer to be opened and closed � Have a touchscreen interface that allows editing of sample and target assignments � Have a touchscreen interface that allows the repeat of a previous run 3 � Have a touchscreen interface that allows users to perform dye calibrations and view results on the touchscreen � Have a touchscreen interface that allows RNase P verification runs and automatically provides pass/fail results � Have a touchscreen interface that allows a user to run a self-verification test � Have a touchscreen interface that allows the user to view the cure QC assessment after calibration and RNase P plate runs are finished � Have a touchscreen interface that allows progress monitoring. 4.1.4 Performance Requirements The instrument shall: � Complete 40-cycle real-time PCR runs using fluorogenic 5� nuclease assay and Fast chemistry in a standard 96-well plate in less than 30 minutes as well as being able to run in standard ramping mode with standard chemistry � Include an OptiFlex system that combines four coupled excitation, and emission filter sets to allow multiplexing up to four unique wavelengths � Support at least two reaction chemistries-TaqMan� Probes and SYBR Green dye � Have real-time quantitative PCR installation specifications to be able to distinguish between 5,000 and 10,000 template copies at a 99.7% confidence level. 4.1.5 Software Requirements The software shall: � Allow flexible customization of run protocols � Allow the creation of secure templates that are locked with a password to limit modification of run setup � Provide run monitoring of connected instruments � Include preoptimized run protocols for applications like standard curve, relative standard curve, relative quantification of gene expression, and SNP genotyping with TaqMan� or SYBR Green chemistries � Include a copy of free software to analyze data for absolute and relative quantification, SNP detection and other assays. � Collect information on both TaqMan and SYBR assays in the same run � Have a genotyping optimization tool that uses real-time data � Provide raw fluorescence data and multicomponent data for troubleshooting � Allow conversion of protocols for the Applied Biosystems StepOnePlus and 7500 to the QuantStudio 3 system protocols � Allow batch analysis of up to 500 experiments simultaneously � Can control the instrument and analyze data from a remote computer on the same network 4 � Provide status monitoring of connected instruments and real-time plots STATEMENT OF NEED (SON) � Have the ability to send user notifications when a run is started, stopped or has an error � Allow registered users of the Connect platform to monitor any connected instrument � Allow users to run an assay plate without performing plate setup prior to beginning of run and to correct plate set up after the run � Allow export of all run results to excel and save graphs as .jpg files � Provide the ability to import a standard curve from one plate for analysis of another plate of the same instrument type � Allow for the selection of endogenous controls for gene expression experiments � Include statistical analysis tools including box-whisker plots, scatter plots and heat maps � Allow preselection of data to export and the location of the exported file � Allow for command-line functions for batch exporting of analyzed run files � Allow users to save predefined analysis settings for auto-exporting run data into format of choice � Contain online high-resolution melt application so user can specify number of clusters/variants and save defined controls and analysis setting. 4.1.6 Instrument Ecosystem The instrument ecosystem shall: � Be part of a desktop ecosystem that consists of a suite of qPCR applications. Instrument data can be grouped (multi-plate) for relative quantitation, SNP genotyping, standard curve, HRM analysis, and presence/absence analysis. � Be part of the Connect platform � Be able to connect to the Connect platform for run setup download and data uploads � Allow a run to be monitored by a web browser or mobile device � Allow batch-analysis of up to 500 experiments simultaneously through an online relative quantitation module � Provide an endogenous control selection tool for gene expression experiments in both the desktop and online quantitation software � Contain statistical anaylsis tools with both the desktop and online quantitation software � Contain a genotyping optimization tool that uses real-time data to pick the best cycle number for calling genotype in both the desktop and online genotyping software � Allow the user to specify number of clusters/variants and save defined controls and analysis settings with both desktop and online software. 4.1.7. Design 5 The system shall: � Have an application programming interface (API) included that allows programming routines to be developed for controlling the instrument � Be cUL, UL, CE, C-TICK, and WEEE compliant � Be developed and manufactured in a facility with quality system requirements that comply with ISO 13485 standards and ISO 9001 standards 4.1.8 Gene expression profiling � Users shall be able to select from more than 61,000 predesigned human, mouse and rat Applied BiosystemsTMTaqMan� Assays. 4.1.9 Able to accept the trade-in of Model STEPONEPLUS. Serial number 272008444 4.1.10 Must be compatible with protocols from Applied Biosystems StepOnePlus Real Time PCR (qPCR) allowing for consistency in experimental set-up 4.2 DELIVERY / INSTALLATION The contractor shall deliver and install the equipment within thirty (30) calendar days after receipt of award.
- Web Link
-
SAM.gov Permalink
(https://sam.gov/workspace/contract/opp/3200a68293ae4a1e8cf459207cf69cc5/view)
- Place of Performance
- Address: Bethesda, MD 20892, USA
- Zip Code: 20892
- Country: USA
- Zip Code: 20892
- Record
- SN07880813-F 20260711/260709230050 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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