SOLICITATION NOTICE
B -- 10X single cell library preparation and sequencing
- Notice Date
- 4/10/2024 5:51:50 AM
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 621511
— Medical Laboratories
- Contracting Office
- NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD 20910-7500 USA
- ZIP Code
- 20910-7500
- Solicitation Number
- 1301146448
- Response Due
- 4/25/2024 9:00:00 AM
- Archive Date
- 05/10/2024
- Point of Contact
- Timothy A. Daniels, Phone: 3014485692
- E-Mail Address
-
timothy.a.daniels18.civ@healty.mil
(timothy.a.daniels18.civ@healty.mil)
- Description
- The requirement is for a custom 10X single cell library preparation and sequencing of 16 samples prepared using the �tissue fixation and dissociation for chromium fixed RNA profiling� protocol (CG000553). Approximately, 7,500-12,500 cells are estimated to be recovered from sequencing. Platforms and instrumentation that conform to expected industry standards and practices are acceptable (e.g., Element AVITI sequencer, 10X Genomics Chromium system). Fast access to sequence data immediately following sequencing and analysis by LIMS or similar system is required. Evaluation Factors: Technical Approach, Past Performance, and price. Evaluation Factors other than price, when combined, are significantly more important than price. Questions and Answers: Please advise if the �tissue fixation� process is to be provided by the Navy Lab, or by the supplier. In other words, will you ship flash-frozen tissue, or provide �fixed� tissue samples? Tissue will be fixed and dissociated using the 10X Genomics �Tissue fixation and Dissociation for Chromium Fixed RNA Profiling� (CG000553) and provided to the vendor for QC, Library Prep, and Sequencing. Please confirm that a read depth of 10,000 reads per cell is adequate. No, sequencing will be completed using standardized methods and platforms with 75bps paired-end reads at 10E5-10E6 raw reads per cell. Alternatively, 100bps pair-end reads at 50E4-50E5 raw reads per cell will suffice. What data delivery is required, i.e. FASTQ files only, or single-cell bioinformatics? ��If bioinformatic analysis is needed, please define the deliverables. Delivery of FASTQ files only is required for this request. No single-cell bioinformatics analysis is requested here. However, vendors are welcome to provide the cost of service without and with 20-40 hours of bioinformatic analysis.
- Web Link
-
SAM.gov Permalink
(https://sam.gov/opp/9acc1d824f38407e8628d10c500deaff/view)
- Place of Performance
- Address: Wright Patterson AFB, OH 45433, USA
- Zip Code: 45433
- Country: USA
- Zip Code: 45433
- Record
- SN07025666-F 20240412/240410230044 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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