Loren Data's SAM Daily™

SAMDAILY.US - ISSUE OF JULY 16, 2023 SAM #7901
SOURCES SOUGHT

66 -- Confocal Microscope System

Notice Date

7/14/2023 7:28:47 AM
 

Notice Type

Sources Sought
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
 

ZIP Code

20892
 

Solicitation Number

75N95023Q00496A
 

Response Due

7/18/2023 12:00:00 PM
 

Archive Date

08/02/2023
 

Point of Contact

Llakim Dubroff, Phone: 3014436677, Joshua T. Lazarus, Phone: 3018276923
 

E-Mail Address

llakim.dubroff@nih.gov, Josh.Lazarus@nih.gov
(llakim.dubroff@nih.gov, Josh.Lazarus@nih.gov)
 

Description

This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified business sources; (2) determine whether they are small businesses (e.g., 8(a), Veteran-owned small businesses, service-disabled Veteran-owned small businesses, HUBZone small businesses, small disadvantaged businesses, and women-owned small businesses); and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your response to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is considered a small business under the applicable NAICS code should indicate its small business status in its response. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Small businesses are encouraged to respond. For equipment/supply requirements, responses must include the place of manufacturing (i.e. address if supply/equipment is a domestic end product and include country of manufacture). For equipment/supply requirements, small businesses must also address the size status of the manufacturer under the applicable NAICS code (i.e. address Non-Manufacturer Rule). Statement of Need and Purpose: The NIDA IRP Confocal and Electron Microscopy Core (CEMC) serves NIDA-IRP researchers by providing access to confocal and electron microscopic technologies to characterize cellular and ultrastructural properties of organelles, tissue culture and brain tissue. Towards this end, the CEMC has established three goals: (1) increase access to confocal and electron microscopic techniques to NIDA-IRP researchers; (2) develop and adapt emerging confocal and electron microscopic techniques for NIDA-IRP researchers; and (3) provide consultations and training to NIDA-IRP researchers in the adaptation and execution of confocal and electron microscopic techniques and procedures. Due to the high demand of confocal microscopy across the IRP and technology advancement over the last 5 years, the IRP requires a new confocal microscope system, that allows users to collect high resolution images of up to� 12 individual labels (colors) and is compatible with the IRP�s existing ZEISS LSM880 with Airyscan confocal microscope to allow for continuity of data collection across systems. Purchase Description: NIDA requires a brand-name or equal Confocal Microscope System with two cutting edge detection units that can be used simultaneously or individually to allow for either super resolution down to 90nm in XY with an innovative 32 channel GaAsP PMT array concept. Each detection channel functions as single very small pinhole of 0.2 AU to increase resolution, while the overall detector captures 1.25 AU and increases signal-to-noise by 4-8 times over traditional GaAsP PMT based confocal systems.� The unique 32-Channel GaAsP PMT area detector must have a Zoom optic to adapt illumination for different objective lenses and acquisition modes.� Must include a micro lens � array that provides a light fill factor of > 95%. Filter wheel with up to 7 emission filters, each providing dual detection windows for fast multitrack imaging.� Combination with secondary beam splitters expand the usability for multicolor sample. With the ZEN Sample Navigator, it is easy to perform a low power overview scan with autofocus function using a cooled monochromatic Axiocam.� The overview image is used for sample navigation and identification of regions of interest. Salient characteristics: NIDA requires a Confocal Microscope System with the following brand-name or equal salient characteristics: The 32+2-Channel Quasar Detection Unit, consists of a calibrated 32 channel multi-element GaAsP detector with typical QE >45% (peak) and two flanking single multi-alkali PMT detectors The 32 channel Quasar GaAsP detector includes micro lenses in front of the individual detector-elements for improved light efficiency. Individual (master) gains for the side PMT�s for the most extreme dyes, plus individual digital gains for the 32 GaAsP channels. The dynamic range allows to work with a unified master gain level. The 32 channel multi-element GaAsP detector, exclusively for Carl Zeiss Microscopy GmbH, shows very low dark noise and allows for spectral binning of individual channels without sacrificing signal/noise ratio. The 10? TwinGate main beam splitter arrangement in combination with the Quasar detection unit leads to superb laser suppression (OD typically >7). The TwinGate main beam splitter arrangement suppresses laser light optimally. By using dichroics and reflecting the laser light using an angle of 10�, an OD of 7 or greater can be reached when using the TwinGate in combination with the Quasar detection unit. As such, virtually all excitation light is reflected, no laser light will reach the detectors and effectively all emission light can be detected; even more, it is possible to detect emission light emerging from less than 10nm away from the laser - which is a unique feature. The freedom to select emission bands for detection, omitting secondary dichroics & emission filters, is possible - even over laser lines due to the effective laser blocking. The highly efficient holographic grating leads to an even spectral dispersion over the entire visual spectral range (380-750 nm).� Grating utilizes a �spectral recycling loop� to recover non-separated light, redirecting it onto the holographic grating for optimum sensitivity. Software integrated linear modulation of laser power from 0.001 to 100% for all laser lines between 458 and 633nm is supported and can be critical to reach the low light level imaging powers that are required for sensitive experiments. Upright Motorized Stand Upright microscope stand with fully motorized 6 position compatible objective nosepiece and 10 position push and click motorized fluorescence filter turret Motorized integrated Z focus with a step size of 10nm and travel range of 25mm Motorized X,Y stage with high accuracy and reproducibility. Stage can be controlled via software and joystick control. TFT touch panel independent of microscope stand with focus control and access to all microscope motorized functions. Computer controlled LED fluorescence illuminator with variable intensity output. With LEDs from 385- 735.� High efficiency hard coated shift free filters for GFP, CY3, DAPI for standard visual fluorescence Imaging. High resolution scanning stage with joystick and integrated computer control including specimen holder suitable for 2 slides. Integrated contrast manager to preset all light and microscope conditions Spectral Imaging and Advanced Linear Unmixing Options Gain calibration and linearization of the 32-element array GaAsP detector and the two flanking multi-alkali PMTs (referred to as the Quasar Detection Unit) allows for all 34-channels to be used for spectral acquisition and analysis. Fast, parallel acquisition of the entire spectral range as a lambda stack (370 � 760 nm) in one single scan (up to 5 frames per second at 512 x 512) covered by the 32+2-Channel Quasar. (Suitable for spectral analysis in living cells, e.g. colocalization studies). More than 10 different fluorescent dyes can be imaged and separated simultaneously. Due to parallel acquisition, no emission light is lost leading to superior detection efficiency when compared to sequential spectral scanning. Spectral resolution of the array GaAsP detector (32-channels) is 8.9 nm when acquiring a simultaneous, parallel spectral image. Further binning of detector elements possible up to 34,4 spectral resolution. Reproducible high-resolution spectral data acquisition with 2,9 nm or 4.3 nm steps is possible through sequential spectral scanning. Emission Fingerprinting can be performed on data acquired in channel mode. Spectral data can be stored in the spectral database and later used for reference-based linear unmixing. Spectral bandwidth for lambda stack acquisition can be adjusted by the user. A full spectral image is used for linear unmixing (suitable for spectral imaging of living cells and later unmixing without any spectral distortions). Emission Fingerprinting: Reliable separation of different fluorescent dyes even with highly overlapping emission spectra (e.g. simultaneous detection and separation of FITC, GFP, YFP, and autofluorescence) based upon known, previously recorded reference spectra. The separated dyes (crosstalk free) are displayed in separate dye- channel images along with the option to display a merged image. Advanced Tools for Emission Fingerprinting/ Linear Unmixing: Weighted unmixing: improved unmixing algorithm for better unmixing results. Channel with Residuals displays the uncertainties of the unmixing result. Automatic component extraction (ACE) of spectra from a lambda stack for a one- click identification of possible reference spectra. Free combination of manually defined reference spectra, automatically extracted reference spectra, and reference spectra stored within the database can be used for performing Linear Unmixing on a lambda stack. Reference spectra can be stored in a database and later recalled. Spectral information is stored automatically with each unmixed image for traceable and reproducible workflow and unmixing experiments. Online Fingerprinting: Image channels are not defined by the traditional detected emission bands but rather by referenced spectra. On-the-fly online unmixing occurs using these referenced spectra to linear unmix the emission bands of overlapping dyes during the image acquisition. Objectives and DIC Objective Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm) Objective Plan-Apochromat 40x Oil Plan-Apochromat 63x/1.40 Oil DIC f/ELYRA EC Plan-Neofluar 5x/0.16 WD=18.5 M27 EC Plan-Neofluar 10x/0.30 WD=5.2 M27 DIC Slider EC PN 10x 20x/PA 20x DIC Slider PA 63x/1.4 III HR Condsr Prism DIC II/0.9 Condsr Prism DIC III/0.9 Polarizer w/color filter carrier Analyzer module DIC ACR P&C shift free Sample navigator Lasers Lasers from 405 nm to 639 nm can be combined.� In addition, this system is equipped with a 730nm NIR laser. For experiments, each emission color is captured with the most suited detector technology along the complete wavelength range from 380 nm up to 900 nm. Camera for Overview Imaging Camera Axiocam 702 mono is a high performance microscopy camera which includes driver software 64bit, USB 3.0 PCIe x1 interface, dual USB 3.0/USB 2.0 cable 3 m and BK7 protection glass (coated). Number of Pixels: 1920 (H) x 1216 (V) = 2.3 Mega Pixels Pixel size: 5.86 �m x 5.86 �m Chip size: 11.3 mm x 7.1 mm, equivalent to 1/1.2"" (13.3 mm diagonal) Spectral range: With protection glass app. 350 nm to 1000 nm Quantum Efficiency 72% at 520 nm (including sensor cover slip) Max. Selectable Resolution: H x V Binning Faktor (digital) 1920 x 1216 1x1 basic pixel count 960 x 608 2x2 640 x 404 3x3 480 x 304 4x4 384 x 240 5x5 Live frame rates (depending on hardware and software configuration): Exp. Time 1ms, H x V Frame Rate 1936 x 1460 >100 fps Control signals: galvanic isolated I/O signals for exposure time, readout time, trigger ready, (i.e. for controlling external mechanical shutters), one trigger input for exposure control, 5V auxiliary voltage, GND Interface: USB 3.0, 300 MBytes/s, 3 m double-USB cable with USB 3.0/USB 2.0, Operating Systems: Windows 10 x64 Prof./Ultimate SP1 (ZEN 2 lite/pro/system) 224 C-Mount Adapter 60N C 1"" 1.0x Computer Microscopy Workstation Premium hp Z6 G4 Rev. 2 (O) - HP Z6 G4 Rev. 2 Workstation Windows 10 LTSC 2019 LSM 9 (O) System - Chipset: Intel C622 - Memory: max. 192 GB RAM - Memory Expansion Slots: 6 x DDR4, RDIMM, ECC, 2933 MHz Modules - PCI Express Connectors: 2 x PCI Express Generation 3 x16 1 x PCI Express Generation 3 x8 3 x PCI Express Generation 3 x4 - SSD: 1 x 512 GB M.2 NVMe - Hard Drives: 2 x 6 TB SATA 7200 rpm (configured as 6 TB RAID 1 hard drive) - Optical Storage: DVD +/- RW recorder for rewriteable - Power Supply: 100 - 127 V / 200 - 240 V / 50 - 60 Hz / 1000 W with country specific plug - Chassis Dimensions: (H x W x D): 445 x 169 x 465 mm - Rescue kit Software Features: ZEN 3.8 System Self-Maintenance Tool, an easy-to-use software along with a calibration objective for maintaining optimal system performance by automated calibration routines. Smart Setup: Easy hardware control - adapts system configuration according to chosen dyes from a large database of fluorochromes. Different acquisition modes are suggested (e.g. Fastest or Best Signal). 64-Bit operating system for efficient data handling and memory management of large datasets. Software programmability and automation with control over all image acquisition functions via high level computer language (VBA 6.4). Innovative concept for reproducing experiments in multi-user environments via the REUSE function which allows reactivation of all acquisition parameters necessary to reproduce an experiment, stored within each image. User-specific Workspace setups can be stored within the ZEN software according to the user�s needs and applications. Lambda Scanning Mode and Linear Unmixing are fully integrated into ZEN standard software. The Windows user login defines the user-specific LSM environment. Each user can have individual settings and will not disturb the other users. Easy administration via Windows. Included Modules integrated 3D module permits innovative 3D renderings of the data. The Experiment Designer (MTS Experiments Macro) module permits heterogeneous complex, combined Time Series with changing acquisition configurations, autofocus, and bleach functions in any order. With Tiles & Positions module advanced tile scan options are available and neighboring, overlapping image stacks in reflection and fluorescent mode can be acquired and stitched together. Imaging up to 100x100 tiles is possible. Processing of single images, Z-Stacks, Time Series, and Tiled images with the Shading Correction tool is available. Advanced processing via our segmentation analysis Built in Deconvolution Direct processing of Data for on the fly deconvolution and Airyscan processing Additional Software license key for offline processing � a floating license. Airyscan 2 Super Resolution Detection Airyscan 2 is a detector for LSM 980 systems, comprising of 32-Channel GaAsP area detector Zoom optic to adapt illumination for different objective lenses and acquisition modes a microlens � array that provides a light fillfactor of > 95%. Different Modes of operation can be chosen: Superresolution (SR and Confocal sampling), and Multiplex modes (4Y, 8Y, see below). The Airyscan detector can be used as well as an additional GaAsP confocal detector. The Superresolution mode allows to increase the image resolution in all three dimensions. This resolution increase comes with an inherent improvement of the signal to noise ratio. 3D data: Lateral resolution of up to 120 nm and axial resolution of 350 nm at 488 nm excitation.��������� 2D data: Lateral resolution of up to 120 nm at 488 nm excitation. 4-8 x increased sensitivity (compared to GaAsP-PMT detector of traditional confocal at 1 AU pinhole opening) Now with multiplex mode we look at speeds up to 47 fps at 512x 512. Confocal mode uses Airyscan 2 as an additional GaAsP detection channel in addition to the Quasar detector. Multiplex mode SR-4Y is easily accessible via a single button, that adjusts sampling and pixel size. In Multiplex mode 8Y, sampling and therefore achievable resolution is continuously adjustable from Confocal (1x Nyquist) to Superresolution (2x Nyquist). For ease of use, 2 sampling options are easily set with single buttons: Superresoultion and Confocal. This allows flexibility to setup the conditions for the experiment, depending on which resolution or acquisition speed is needed. The 4-8x enhanced Signal to Noise is preserved for high speed acquisition in Multiplex mode. Any fluorescent dye can be used for imaging, no changes in sample preparation technique are necessary. Laser line flexibility from 405 nm to 639 nm is available for covering the entire visible spectrum utilizing the lasers available from your LSM 980. Excitation with a multiphoton laser and detection with Airyscan 2 in all modes is possible. Acquisition strategies: Airyscan 2 can be used for all available multidimensional acquisition setups and the available combinations in ZEN software, e.g.: z-stack, multicolor, multiple positions, tiling, ROI, bleaching and photomanipulation experiments, and time-series. Airtable Include 30x36 Table, RP,I325 Protect Premium Service (4 option years, 5 years total of coverage) The complete system will have a 12-month warranty. Under this warranty policy the travel and labor necessary to repair the instrument and all parts are provided by vendor at no cost to the customer. All service performed on this equipment shall be provided by certified and factory trained technicians. Contractor shall provide any required replacement components due to malfunctions on-site and will include all travel, parts and labor. Coverage for: Research Microscope motorized stage, Airyscan, LSM980 System, 405/440nm CW Laser, NIR Laser and NIR Detector One (1)* preventative maintenance inspection per agreement year. This includes all labor and travel associated with conducting this PM inspection visit. - All labor and travel related charges associated with any service visit in addition to the PM visit as may be required during the contract year, is included. - All covered replacement parts - #Scheduled and emergency service performed between the hours of 8:00 AM and 5:00 PM Monday through Friday excluding contractor holidays - Factory trained Field Service Engineers - Software Version upgrades available on an �as-issued� basis and will be offered upon request The software Version upgrade will pertain to minor version upgrades (i.e. 2.0 to 2.1) and do not include platform upgrades (i.e. AIM to ZEN or ZEN 2.X to ZEN 3.Y) The software installation will be covered under the service agreement, however standard time and material rates will apply for the installation of software upgrades to all off-line workstations Quantity: One (1) Confocal Microscope System. Delivery Date: �The contractor shall deliver and install the required equipment within (16) sixteen weeks after receipt of order. On-site training must take place at the customer site within 1 week of equipment installation. Performance will be monitored by Confocal and Electron Microscopy Core staff. Instrument delivery and installation will be provided by qualified personnel to ensure that the instrument is properly installed and ready to use. This includes the physical setup, calibration and performance verification of the system. Initial customer training performed by certified and factory trained personnel. Period of Performance: 1 year warranty on all parts, labor and travel (including 1 Preventative Maintenance service visit) will start on date of delivery and acceptance. This requirement includes the use of options to extend the period of performance for four (4) additional 12-month option periods. Option(s): 4 option years for extended warranty Option Year 1 will include extended warranty. Option Year 2 will include extended warranty. Option Year 3 will include extended warranty. Option Year 4 will include extended warranty. Capability Statement /Information Sought: Companies that believe they possess the capabilities to provide the required products or services should submit documentation of their ability to meet each of the project requirements to the Contracting Officer. The capability statement must specifically address each of the project requirements separately.� Additionally, the capability statement should include 1) the total number of employees, 2) the professional qualifications of personnel as it relates to the requirements outlined, 3) any contractor GSA Schedule contracts and/or other government-wide acquisition contracts (GWACs) by which all of the requirements may be met, if applicable, and 4) any other information considered relevant to this program. Capability statements must also include the Company Name, Unique Entity ID from SAM.gov, Physical Address, and Point of Contact Information. The response must include the respondents� technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. Interested companies are required to identify their type of business, applicable North American Industry Classification System (NAICS) Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number. Submission Instructions: The response is limited to ten (10) page limit.� The 10-page limit does not include the cover page, executive summary, or references, if requested. All responses to this notice must be submitted electronically to the Contracting Officer Identified below. The response must be received on or before July 18, 2023, at 3:00 pm EST. Facsimile responses are NOT accepted. Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in www.sam.gov. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).� Closing Statement: THIS NOTICE IS NOT A REQUEST FOR COMPETITIVE PROPOSALS. However, interested parties may identify their interest and capability to respond to this notice. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. All responsible sources may submit a capability statement, proposal, or quotation which shall be considered by the agency. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Unique Entity ID (UEI), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov. All responses must be received by the closing date and time of this announcement and must reference the solicitation number, 75N95023Q00496A. Responses must be submitted electronically to Llakim Dubroff, Contract Specialist, at Llakim.Dubroff@nih.gov U.S. Mail and Fax responses will not be accepted. Primary Point of Contact: Llakim Dubroff, Contract Specialist
 

Web Link

SAM.gov Permalink
(https://sam.gov/opp/fd777477e9f342e59ee3606ef6ef56f4/view)
 

Place of Performance

Address: Baltimore, MD 21224, USA

Zip Code: 21224

Country: USA
 

Record

SN06749996-F 20230716/230714230046 (samdaily.us)
 

Source

SAM.gov Link to This Notice
(may not be valid after Archive Date)

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