SOLICITATION NOTICE
65 -- SERVICES FOR PROBE DESIGN AND RNASCOPE BRAND REAGENT KITS
- Notice Date
- 4/15/2023 9:57:34 AM
- Notice Type
- Presolicitation
- NAICS
- 325414
— Biological Product (except Diagnostic) Manufacturing
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIAID BETHESDA MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- NOI-NIAID-2169561
- Response Due
- 4/22/2023 1:00:00 PM
- Point of Contact
- DIANA ROHLMAN, Phone: 4063639366, Laura Grey, Phone: 4063759812
- E-Mail Address
-
diana.rohlman@nih.gov, laura.grey@nih.gov
(diana.rohlman@nih.gov, laura.grey@nih.gov)
- Description
- This is a Notice of Intent, not a request for proposal. �The National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) intends to negotiate on an other than full and open competition basis with a Advanced Cell Diagnostics, Newark, CA These services and supplies are required for RNA in situ hybridization. Qty: 23 Bioinformatics service fee for probe designs (Multiple)� Qty: 23 RNASCOPE brand probes (Multiple) Qty: 1 RNASCOPE brand Multiplex reagent kit #323270� Sole Source Justification: Advanced Cell Diagnostics, Inc. (ACD) is the sole supplier of RNAscope� probes and reagent Kits. This projects demands RNA in situ hybridization to validate 23 target markers and visualize the individual cellular clusters identified in the single-cell RNA-seq analysis. RNAscope enables as much as 400-fold improvement over conventional RNA in situ hybridization, resulting in high detection sensitivity, thus RNAscope technology is the best choice to accomplish our goals for this project. RNAscope was designed to amplify target-specific signal without also amplifying the background signal, resulting in marked improvement in signal-to-noise ratio. This is accomplished by manufacture`s patented double Z-probe design for in situ hybridization, where two independent probes (a double Z probe pair) are required to hybridize in tandem to the target sequence for signal amplification to occur. Since it is highly unlikely that two independent probes will hybridize to a nonspecific target right next to each other, this design concept ensures highly selective amplification of target-specific signals, therefore improving both sensitivity and specificity. A Preamplifier (PreAmp) molecule hybridizes to each double Z probe pair, and then multiple Amplifier (AMP) molecules hybridize to each Preamp. Finally, multiple HRP-labeled Label Probes hybridized to each AMP. DAB substrate is added for colorimetric detection of target RNA. Fluorescent- or AP-labeled Label Probes can also be used for fluorescent or Fast Red detection of target RNA. RNAscope Reagent Kits offer easy single-plex, multiplex, and automated detection of virtually any target RNA in situ using formalin-fixed paraffin-embedded tissue sections on slides. The statutory authority for this sole source requirement is 41 U.S.C. 1901 (a) (1) as implemented by FAR 13.106-1 only one responsible source and no other supply of service will satisfy agency requirements. THIS IS NOT A REQUEST FOR PROPOSAL. �All responsible sources that could provide equal services may submit a capability statement that will be considered by email (subject line to reference NOI-NIAID-2169561 to Diana Rohlman at diana.rohlman@nih.gov �by 4:00 pm eastern standard time, April 22, 2023. �All responses received by the closing date of this synopsis will be considered by the Government. �A determination not to compete this requirement, based upon responses to this notice, is solely within the discretion of the Government.
- Web Link
-
SAM.gov Permalink
(https://sam.gov/opp/b7db18b173884b3cb40872b9f671380e/view)
- Place of Performance
- Address: Hamilton, MT 59840, USA
- Zip Code: 59840
- Country: USA
- Zip Code: 59840
- Record
- SN06651905-F 20230417/230415230104 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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