SOURCES SOUGHT
B -- Next Generation RNA Sequencing
- Notice Date
- 8/12/2020 12:28:32 PM
- Notice Type
- Sources Sought
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- USDA ARS SEA AAO ACQ/PER PROP STONEVILLE MS 38776 USA
- ZIP Code
- 38776
- Solicitation Number
- 1011698
- Response Due
- 8/17/2020 11:00:00 AM
- Archive Date
- 09/01/2020
- Point of Contact
- Terry Krutz, Phone: 6626863011
- E-Mail Address
-
terry.krutz@usda.gov
(terry.krutz@usda.gov)
- Small Business Set-Aside
- SBA Total Small Business Set-Aside (FAR 19.5)
- Description
- RNA Sequencing Service �Statement of Work and Minimum Requirements for Turkey Sperm Storage Tubule (SST) Tissues The requested quote for RNA sequencing of 50 turkey SST samples covers all aspects of service, including RNA quality assessment, library preparation, library quality assessment, and sequencing. Specifically, RNA extracted from laser capture micro-dissected samples will be evaluated for quality through bioanalyzer analysis. High quality RNA will be used to generate libraries using a library generation kit that is ideal for low concentrations of RNA and for the enrichment of messenger RNA; this is a minimum requirement due to the limited numbers of cells in the samples and the high levels of ribosomal RNA in SST samples. Additionally, any samples that are deemed of a lower quality can be replaced prior to moving on to library preparation, guaranteeing only high-quality samples will be sequenced. Next Generation Sequencing will be completed using the Illumina NovaSeq (300 cycles) with a read length of 2 x 150 (Paired End) allocated over 1.5 lanes, with a guaranteed number of 50 million paired-end reads (25M reads in each direction) pass filter reads per sample. The guaranteed turnaround time from the project start date is 6 weeks; this is a minimum requirement.� The company has specialized experience with all aspects of RNA sequencing; this is a minimum requirement. Provide the following unique service aspects that we require to process and analyze our samples: 1. Communication of sample and library quality checks with the sequencing core at each step of the process; 2. The use of a RNA sequencing library generation kit that is stranded and enriched for messenger RNA; 3. The ability to replace any RNA samples that did not reach the core's minimum standards prior to sequencing; 4. Guaranteed number of 50 million paired-end reads (25M reads in each direction) pass filter reads per sample; 5. Access to the Illumina NovaSeq Platform, which allows high-throughput yet cost-effective sequencing; 6. Guaranteed turnaround time from the project start date of 6 weeks; 7. A low cost for the project considering the number of samples and the sequencing platform chosen.
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/d25db51e5bc2413bb9a4439db0c89d05/view)
- Place of Performance
- Address: Beltsville, MD 20705, USA
- Zip Code: 20705
- Country: USA
- Zip Code: 20705
- Record
- SN05755539-F 20200814/200812230150 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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