SPECIAL NOTICE
66 -- Purchasing a cryo grid plunger for NCI electron microscopy facility
- Notice Date
- 7/29/2020 11:46:16 AM
- Notice Type
- Special Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- NIH National Cancer Institute Rockville MD 20850 USA
- ZIP Code
- 20850
- Solicitation Number
- 75N91020Q00086
- Response Due
- 8/8/2020 9:00:00 AM
- Archive Date
- 08/23/2020
- Point of Contact
- William Neal, Phone: 2402765433, Reyes Rodriguez, Phone: 2402765442
- E-Mail Address
-
william.neal@nih.gov, reyes.rodriguez@nih.gov
(william.neal@nih.gov, reyes.rodriguez@nih.gov)
- Description
- Title: �Purchasing a cryo grid plunger for NCI electron microscopy facility Document Type:������������� Special Notice Solicitation Number: ����75N91020Q00086 Posted Date:������������������ ��� 07/29/2020 Response Date:���������������� 08/8/2020 Classification Code:� 6640 � LABORATORY EQUIPMENT AND SUPPLIES������������������������ � NAICS Code:� 334516 � Analytical Laboratory Instrument Manufacturing (1000 employees).��� Contracting Office Address: Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E128, Bethesda, MD� 20892, UNITED STATES. Description: Purpose of procuring the Leica GP2 package, it contains a plunger main console and its accessories.� After delivery, installation of this instrument and necessary training will be scheduled for one day.� The installation engineer will first unpackage the crate.� The Contractor must place the plunger on a stable lab bench in the sample prep room.� When everything is fully assembled, together with the lab and facility staff, they will perform an installation acceptance test, evaluating temperatures of chamber, ethane bath, blotting distance and force, humidity, and the plunger motor mobility.� This purchase is from Leica Microsystems at 1700 Leider Lane Buffalo Grove, IL, 60089-6622 The response close date of this notice for this requirement is in accordance with FAR 5.203(a)(1). This acquisition will be processed under FAR Part 12 � Acquisition for Commercial Items and will be made pursuant to the authority in FAR 13.106-1(b)(1) using simplified acquisition procedures for commercial acquisitions and is exempt from the requirements of FAR Part 6. The North American Industry Classification System code is 334516 � Analytical Laboratory Instrument Manufacturing (1000 employees). This will be awarded as a Severable Firm Fixed price type contract. It has been determined there are no opportunities to acquire green products or services for this procurement. BACKGROUND Protein structure determination using cryo electron microscopy (cryoEM) is becoming very demanding among NIH intramural investigators.� NCI is the leading institute in structural biology specialized cryoEM and has invested great resource to support groundbreaking research.� In order to support an urgent need for cryo grid preparation for NCI investigators in building 37 as well as collaborators in the Bethesda campus, we request to purchase a cryo grid plunger from Leica Microsystems and placed in the sample preparation room near the Titan Krios electron microscope.� Having a plunger nearby significantly benefits the NCI structural biology groups by greatly shorten the time between protein prep and grid preparation.� PRODUCT FEATURES/SALIENT CHARACTERISTICS Cryo plunger is an instrument used in sample preparation in cryo electron microscopy experiments.� This instrument contains a temperature and humidity-controlled sample chamber, liquid ethane bath in a liquid nitrogen dewar, and a computer controlling systems.� The temperature and humidity in the sample chamber are set according to optimal condition of the protein specimen.� Users place a specimen grid on a tweezer and load it onto the robotic rod inside the chamber.� Then a drop of protein specimen is placed on the grid.� The computer control will start a time-controlled plunging sequence: a filter paper blots off the excess liquid and the robotic rod �plunges� the grid into liquid ethane bath for instant vitrification.� When the procedure is completed, the users transfer the frozen grid into a grid storage box in the liquid nitrogen dewar.� Leica Microsystems is a company that poses many advanced products for many microscopy applications.� Leica cryo GP2 is their newest model for preparing grids for cryoEM.� GP2 offers a great advantage for high stability, reproducibility, and flexibility comparing to its competitors.� GP2 has more plunging and blotting parameters for each experiment with different requirements.� For difficult specimen such as fragile protein complex, fibers, and protein with high affinity to filter paper, GP2 is proper instrument for this type of applications. �The following product features/characteristics are required for this requirement: Accurate temperature and humidity control of the sample chamber for high reproducibility and reliability, which other venders cannot compete. GP2 offers gentle blotting, which significantly reduces protein or grid damage by filter papers. GP2 is the only product in the market that offers single-side blotting, which is best for protein with high affinity to filter paper.� The competing products are 2-side blotting. Temperature control for the liquid ethane bath maintains at -180C to ensure instant vitrification. GP2 offers many options and parameters to accommodate different types of specimen and challenging experiments, such as bloting distance. Large capacity liquid nitrogen dewar design to minimize unwanted ice contamination. Liquid nitrogen dewar contains a nitrogen level sensor, which alarms users when refrigenent level is below the required level. Its touch-screen computer control provides user friendly operation interface, simple to operate and versatile for different applications. The system includes a warm up cycle for removing ice contamination between experiments. DELIVERY / INSTALLATION Delivery address: 37 Convent Dr. Rm 2122, Bethesda MD 20892. Installation address: Sample preparation room, 37 Convent Dr. Rm B1036, Bethesda MD 20892. It shall be delivered within 120 days of the purchase order award. ��������������������������������� TRAINING Training is included in this quote.� This plunger is more complicated than the competitive products.� Therefore, initial training is very important to learn the most updated features for this system. Leica Microsystems is a company that has many advanced products for many microscopy applications.� Leica cryo GP2 is their newest model for preparing grids for cryoEM.� GP2 offers a great advantage for high stability, reproducibility, and flexibility comparing to its competitors.� GP2 has more parameters for the experiments.� For difficult specimen such as fragile protein complex, fibers, and protein with high affinity to filter paper, GP2 is proper instrument for this type of applications. Many labs at NIH have already owned this instrument and they all agree about its great performance.� Due to high demand of this application, NCI in Bethesda urgently needs to acquire one for cryo specimen preparation for difficult sample. This notice is not a request for competitive quotation. However, if any interested party, especially small businesses, believes it can meet the above requirement, it may submit a capability statement, proposal, or quotation, which shall be considered by the agency.� The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the NCI to determine if the party can perform the requirement. �Responses must be received in the contracting office by 12:00PM EDT, on August 8, 2020.� All responses and questions must be in writing and faxed (240) 276-5433 or emailed to William Neal, Contracting Officer via electronic mail at william.neal@nih.gov. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, Contractors must be registered and have valid, current Entity Record, including current Representations and Certifications, in the System for Award Management (SAM) through SAM.gov. Reference: 75N91020Q00086 on all correspondence.
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/17ab06e405f2400b87ed526ef09f96ac/view)
- Place of Performance
- Address: Bethesda, MD 20892, USA
- Zip Code: 20892
- Country: USA
- Zip Code: 20892
- Record
- SN05737343-F 20200731/200729230141 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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