SAMDaily.us | SRCSGT | 66 | Procurement of one Becton Dickson FACSympony A5 Flow Cytometer or Equivalent

SAMDAILY.US - ISSUE OF APRIL 09, 2020 SAM #6706
SOURCES SOUGHT

66 -- Procurement of one Becton Dickson FACSympony A5 Flow Cytometer or Equivalent

Notice Date: 4/7/2020 9:03:57 AM
Notice Type: Sources Sought
NAICS: 334516 — Analytical Laboratory Instrument Manufacturing
Contracting Office: NIH National Cancer Institute Rockville MD 20850 USA
ZIP Code: 20850
Solicitation Number: 75N91020Q00051
Response Due: 4/14/2020 10:00:00 AM
Archive Date: 04/29/2020
Point of Contact: Adam Hernandez, Phone: 2402765633
E-Mail Address: adam.hernandez@nih.gov
(adam.hernandez@nih.gov)
Description: This Small Business Sources Sought Notice (SBSS) is for information and planning purposes only and shall not be construed as a solicitation or as an obligation on the part of the National Cancer Institute (NCI). The purpose of this Sources Sought Notice is to identify qualified small business concerns including 8(a), HUBZone or Service-Disabled Veteran-owned business concerns that are interested in and capable of performing the work described herein. The NCI does not intend to award a contract based on responses received nor otherwise pay for the preparation of any information submitted. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This requirement is assigned North American Industry Classification System (NAICS) code 334516 with a size standard of 1,000 employees is being considered. NCI may issue a request for quotation (RFQ) as a result of this Sources Sought Notice. THERE IS NO SOLICITATION AVAILABLE AT THIS TIME. However, should such a requirement materialize, no basis for claims against NCI shall arise as a result of a response to this Sources Sought Notice or the NCI�s use of such information as either part of our evaluation process or in developing specifications for any subsequent requirement. 1.0�BRAND NAME OR EQUAL This requirement is for the procurement of the brand name or equivalent instrument described in section 3.0. The Federal Acquisition Regulation (FAR) provision FAR 52.211-6, Brand Name or Equal (AUG 1999) is applicable to this requirement. 2.0 BACKGROUND The U.S. Department of Health and Human Services (DHHS), National Institutes of Health (NIH), National Cancer Institute (NCI), Center for Cancer Research (CCR), Vaccine Branch (VB) requires the purchase of of one Becton Dickson FACSympony A5 flow cytometer or equivalent. BD Biosciences, the manufacturer of three LSRII flow cytometers in the Vaccine Branch Flow Cytometry Core, has informed the branch that service and support will no longer be available for the LSRII instruments starting in September 2020. It is critical that these instruments be replaced so that the mission of CCR will not be negatively impacted. The NCI Flow Cytometry Core Managers developed a timeline for the replacement of these instruments at NCI. The replacement of this instrument is scheduled to be replaced in Fiscal Year 2020 under that timeline. 3.0 PRODUCT FEATURES/SALIENT CHARACTERISTICS The following product features/characteristics are required. Lasers:�� 488nm 400mw Coherent Sapphire laser �� 628nm 200mw MPB laser �� 405nm 200mw Coherent OBIS laser �� 532nm 200mw Coherent Sapphire laser �� 355nm 60mw Coherent G4 UV laser �� Quantiflash Pulser LED to QC detectors with upgrade kit for � � � � � � � � � � � � � � � � � � � � integration Fluidics:�� Integrated� System High Throughput:�� 96 well sample loading system Detectors:�� 30 parameters (FSC, SSC and 28 Flurorescent) �� 488nm: BB515, BB630, BB660, BB700, BB790 �� 628nm: APC, Alexa 700, APC-Cy7 405nm:�� BV421, BV480, BV570, BV605, BV650, BV711, BV750, BV786 532nm�� PE, PE-CF596, PE -Cy5, PE-Cy7 355nm:�� BUV395, DAPI, BUV496, BUV563, BUV615, BUV661, BUV737, BUV805 The instrument shall: Have the capability of up to 10 lasers, 50 parameters with 48 colors to future-proof the instrument so that it can be upgraded to handle future reagents and application needs. Use state of the art fiber optics to carry the light/fluorescent signal from the flow cell to the photomultiplier tube detectors (PMTs) to minimize noise in each channel. Have option of tube, APD or Silicon Chip detectors based on maximum channel sensitivity with each type to optimize each channel for the best signal to noise ratio. Have the Decagon optical arrangement for 5 lasers to allow�for the detection of up to 10 fluorochromes per laser and is compatible with current and future reagents. Have air-cooled lasers and not have special room requirements (no special electrical requirements, no special plumbing requirements, and no special air requirements). The facility is not equipped for water cooled lasers so all lasers shall be air-cooled.� Also the facility is not set up for special electrical or plumbing requirements. Have fixed alignment optics in conjunction with solid state lasers ranging in power from 60mW UV to 1000mW 532nm. Fixed alignement lasers allows for more consistency between experiments and since many experiments in this facility run for more than 1 year, this is a requirement. Have easily replaceable optical filters and mirrors for experimental flexibility. This is a requirement for any Core instrument to have the flexibility to handle different fluorochromes for different applications. Have sensitivity of <30 MESF-PE and <80 MESF-FITC. This sensitivity range is required to be compatible with all ongoing experiments. Provide for performing real-time statistics via the integrated software to monitor experimental samples during acquisition. Have software for standardizing and Quality Control (QC) of the instrument. This software must automate the QC of numerous parameters and alerts the user if a parameter does not meet specification and tracks the QC parameters over time. Upgrade to a LED Integrated Pulser QC tool shall be optional. Having quality control built in to the software is needed to standardize experimental samples that are acquired at different times (many months apart) during long-term experiments. Be capable of analyzing at least 30 logical gates. The nature of the vaccine samples often run on the instrument requires the analysis of rare populations of cells that require many markers to define. Have state of the art low noise digital acquisition electronics with acquisition speeds of 40000 events per second. These acquisition speeds are required to process samples that are acquired at over 1 million events per data file. Have the capability to sample from 96 well microtiter plates with an automated sampler. The 96 well automated sampler shall have the ability to run a 96 well plate in the high throughput mode is less than 15 minutes. One assay that is run frequently in the Core is an ADCC assay that is stained and run out of 96 well plates. The instrument shall have the ability to run this assay. Have the option of adding a Forward Scatter PMT for detection limits of .2 microns. One of the Principal Investigators using the Core studies extracellular vesicles and the instrument must have the ability to run small particles. Have the software capability of Laser Control Software which allows the user to adjust laser power up or down on an as needed basis since different experiments have different fluorochromes with different laser requirements. Be software compatible with existing laboratory and institute high parameter cytometers that are manufactured by BD Biosciences for ease of transition of existing experimental protocols and minimum operator training. The FACS Core currently has 2 BD FACSymphony A5 analyzers 1 BD LSRII analyzer and a BD FACSAriaIII cell sorter. Include a HP Z2 tower G4 workstation and operates using Windows 10 to run the instrument and software. Include reagents to evaluate and determine optimal instrument settings including: A high parameter immunology panel of human antibody reagents for instrument setup A prototype fluorochrome evaluation kit with human antibody reagents for evaluating detectors where non-standard reagents can be used (BB630, BB660, BB790, BUV615) These reagents are critical to instrument setup for this core facility. Similar reagents including the proprietary reagents, BB630, BB660, BB790 and BUV615 are currently being used in existing experimental panels. These reagents will allow this instrument to be setup in the same manner as the two existing FACSymphony A5 instruments in the core. Include reagents to assist in designing and setting up high parameter staining panels including: A high parameter reagent design kit with access to 150 small scale fluorochrome conjugated antibodies, including proprietary fluorochromes These reagents will be used to setup future staining panels that are compatible with existing protocols. 3.1 DELIVERY / INSTALLATION Delivery shall be within 120 business days of purchase order award. All shipping/handling (including FOB) and delivery/installation fees shall be included in the quote. Upon delivery, the Contractor shall notify the NCI Contracting Officer Representative (COR)�to schedule the installation date and time that shall occur within 5 business days after delivery. Installation shall be performed by, or under the direct supervision of, a certified operator. The Contractor shall deliver and install the equipment at the following address: 41 Medlars Drive Room C310 Bethesda, MD 20892 3.2 TRAINING Within 14 business days after installation, at least one day of training will be provided on-site for at least one primary user of the instrument. All training expenses, including materials and/or travel expenses, shall be included in the quoted price. 3.3 WARRANTY A minimum of 12-months warranty from the date of installation shall be included for each instrument, which covers the cost of repair and/or replacement, including labor or any defect in workmanship or parts. At least one preventative maintenance visit during the warranty period shall be provided for each instrument. Phone support, remote diagnostics and troubleshooting, and field service shall be included with the warranty and shall have a 48-hour on-site turnaround time by a designated service engineer. 4.0 RESPONSE DELIVERY POINT Capability statements shall be submitted via email to Contract Specialist, Adam Hernandez, at adam.hernandez@nih.gov no later than 1:00 P.M. EST on Tuesday, April 14, 2020 (04/14/2020). All information furnished must be in writing and must contain enough detail to allow the NCI to determine if it can meet the unique specifications described herein. A determination by the Government not to compete this requirement based upon responses to this notice is solely within the discretion of the Government.�Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award, contractors must have valid registration and certification in the System for Awards Management (SAM) at www.sam.gov. No collect calls will be accepted.�Please reference number 75N91020Q00051 on all correspondence. This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, an RFQ may be published. However, responses to this notice will not be considered adequate responses to a solicitation(s).
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66 -- Procurement of one Becton Dickson FACSympony A5 Flow Cytometer or Equivalent