SOLICITATION NOTICE
A -- Histology and Immunohistochemistry for Brain Injury
- Notice Date
- 3/13/2018
- Notice Type
- Presolicitation
- NAICS
- 541715
— Research and Development in the Physical, Engineering, and Life Sciences (except Nanotechnology and Biotechnology)
- Contracting Office
- Department of the Army, U.S. Army Medical Research Acquisition Activity, U.S. Army Medical Research Acquisition Activity, Attn: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014, Maryland, 21702-5014, United States
- ZIP Code
- 21702-5014
- Solicitation Number
- W81XWH-18-R-0027_Presolicitation
- Archive Date
- 4/12/2018
- Point of Contact
- Sharew Hailu,
- E-Mail Address
-
sharew.hailu.civ@mail.mil
(sharew.hailu.civ@mail.mil)
- Small Business Set-Aside
- N/A
- Description
- THIS IS A COMMERICIAL NOTICE OF INTENT, NOT A REQUEST FOR PROPOSAL. The Brain Trauma Neuroprotection and Neurorestoration (BTNN) Branch at Walter Reed Army Institute of Research (WRAIR) conducts preclinical research identifying the pathophysiology processes of mild-severe traumatic brain injury (TBI), as well as, evaluating novel therapeutic strategies to diagnose and/or treat TBI occurring in military centric environments. The Blast-induced Neurotrauma (BINT) Branch use models to validate and characterize scaled experimental blast exposure conditions and assessments of outcomes relevant to blast-injured Warfighters. Investigators in this program conduct basic and applied research on the mechanisms of primary and secondary injury resulting from exposure to blast. Histopathology and immunohistochemistry support services for the Brain Trauma, Neuroprotection and Neurorestoration Branch and Blast-inducted Neurotrauma, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research (WRAIR). The Contractor representative shall secure the provisions necessary to meet the government (BTNN, BINT) objectives and perform the tasks. The Government will use a single-award, IDIQ contract type. The contractor shall deliver prepared, processed, and stained tissue each task listed below within 2-3 weeks after the contractor receives a Task Order with instructions from the government (BTNN and BINT). 5.1.Preparation and Processing Rat, ferret, swine, and/or non-human primate brain or peripheral organs (including liver, kidney, heart, lung, urinary bladder and muscles) preparation for histological or immunohistochemicaly processing. (The cerebrum, cerebellum, brain stem, and olfactory bulbs are processed separately). 5.2.Hematoxylin & Eosin or Nissl Staining Hematoxylin & Eosin (H&E) or Nissl staining of rat, ferret, swine, and/or non-human primate brain or organ tissues for evaluation of gross histopathology. The brain samples to be processed under this task are approximately 800 annually. 5.2.1.Upon receipt of instructions f rom the government, the contractor shall perform H&E staining or Nissl on selected series of brain or organ sections prepared under Task 5.1 in accordance with standard or approved protocols and coverslip for delivery. 5.3.Colorimetric Immunohistochemical and Immunofluorescence Staining The contractor shall provide colorimetric Immunohistochemical and immunofluorescence staining of rat, ferret, swine, and/or non-human primate brain or organ tissues for evaluation of cellular and molecular changes in the brain or peripheral organs after brain injuries. The tissue samples to be processed under this task are approximately 500 annually. 5.3.1.Upon receipt of instructions f rom the government, the contractor shall preform colorimetric immunohistochemical staining and immunofluorescence staining labeling (including immunofluorescence double or triple labeling as needed) on selected free-floating brain sections or paraffin-embedded organ sections prepared under Task 5.1 using standard or approved immunohistochemistry protocols and techniques to stain or label cellular and molecular markers for different aspects of brain or organ injuries. After staining each section, the contractor shall mount the stained sections on slides (2 sections per slide) and coverslip for delivery. The antibodies to be used for this task will be determined by the government as studies proceed. The contractor shall perform the following tasks: a.The contractor shall perform immunohistochemical staining on brain sections (cerebrum, cerebella, brain stem, and olfactory bulb) or paraffin-embedded organ sections prepared under Task 5.1.1 - 5.1.2 with 1 primary antibody. b.The contractor shall perform immunofluorescence labeling (including double or triple labeling) on brain sections (cerebrum, cerebella, brain stem and olfactory bulb) or paraffin-embedded organ sections prepared under Task 5.1.1 - 5.1.2. c.If necessary, the contractor shall perform testing and titration of primary antibodies for colorimetric and immunofluorescence staining requested by the government, using selected brain sections or organs prepared under task 5.1. 5.4.Other Neurohistological Staining Other neurohistological staining may be performed to detect cellular and molecular markers in the brain after brain injuries upon receipt of instructions from the government. The brain samples to be processed under this task are approximately 200 annually. 5.4.1.The contractor shall perform histochemical stains, on selected free-floating brain sections prepared under Task 5.1 using standard or approved protocols and techniques to stain or label cellular and molecular markers of different aspects of brain injuries. After staining, the contractor shall mount the stained sections on slides with a coverslip for delivery. The markers to be used for this task will be determined by the government as studies proceed. 5.4.2.The contractor shall perform Amyloid Plaque detection using Hirano Zimmerman method in the rat, ferret, swine, and/or non-human primate brains using the selected silver stained sections from task 5.4.1. After staining, the contractor shall mount the stained sections on slides with a coverslip for delivery. 5.4.3.The contractor shall perform Golgi staining on freshly collected rat, ferret, swine, and/or non-human primate brains. The contractor will provide all the necessary reagents that is required for processing the freshly dissected brains at the government site. The contractor will deliver ready to view slides after sectioning, staining and cover slipping the Golgi-Cox impregnated brain blocks. 5.4.4.The contractor shall perform neuronal apoptosis detection with in situ DNA nick end-labeling technique (TUNEL) in selected sections from Task 5.1.1. The government will provide unfixed-fresh frozen tissue and the contractor will section, stain and deliver ready to view TUNEL stained slides. 5.4.5.The contractor shall perform Fluoro-Jade staining in selected sections from Task 5.1.1. The government will provide brain tissue processed according to the companies protocol and the contractor will section, stain and deliver Fluoro-Jade stained slides. 5.4.6.The contractor shall perform Timm's staining in selected sections from Task 5.1.1. The government will provide brain tissue processed according to the companies protocol and the contractor will section, stain and deliver Timm stained slides. 5.4.7.The contractor shall perform histological examination of mitochondrial enzymes or other mitochondria specific metabolic / oxidative / inflammatory biomarkers assays to detect in situ enzymatic activity / content in selected sections from Task 5.1.1. The government will provide unfixed-fresh frozen tissue and the contractor will section, stain and deliver ready to view mitochondria enzyme activity-stained slides. 5.5.Shipping and Handling The contractor shall deliver the processed brain slides in slide boxes by commercial carriers or in person to the government facility outlined at the Task Order level. 5.6.Quality Control The Offeror shall submit a Quality Control Plan (QCP). At a minimum, the QCP must include a self-inspection plan, an internal staffing plan, and an outline of the procedures that the Offeror will use to maintain quality, timeliness, responsiveness, customer satisfaction, and any other requirements set forth in this solicitation. The QCP must include how it will ensure a high level of quality for all work performed in support of the performance work statement (PWS) and all deliverables. The QCP must also explain how the Offeror will handle any issues should they arise that include but are not limited to (1) proper tissue processing variables that must be addressed in order for the outcome to be consistent and free of artifacts and (2) incomplete fixation, which in turn may lead to improper dehydration, clearing, and infiltration. The QCP shall address potential issues that may arise when initial samples are sent to the laboratory, stored, and returned to the WRAIR scientists and meet the Quality Standards identified in the PWS related to Pathology and Histological standards and Staining standards. It is anticipated that solicitation W81XWH-18-R-0027 will be electronically available on or about March 30, 2018 in draft form. The RFP may be accessed through the FedBizOpps website, https://www.fbo.gov. All responsible sources may submit proposals. This is not a request for proposals. This requirement is defined as code 541715 under the North American Industry Classification System (NAICS).
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USA/USAMRAA/DAMD17/W81XWH-18-R-0027_Presolicitation/listing.html)
- Place of Performance
- Address: WRAIR, Silver Spring, Maryland, United States
- Record
- SN04853100-W 20180315/180313231735-7fc4cec1e457d2ffc75c288c303fb5f4 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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