DOCUMENT
65 -- Intent to Sole Source (ISS/Sources Sought) Becton, Dickinson and Co. 646500 BD Influx Flow Cytometer Cell Sorter for the VA Bronx Medical Center, Bronx, NY. - Attachment
- Notice Date
- 3/13/2018
- Notice Type
- Attachment
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- US Department of Veterans Affairs;Veterans Health Administration;Service Area Office (SAO) East;323 North Shore Drive, Suite 500;Pittsburgh PA 15212-5319
- ZIP Code
- 15212-5319
- Solicitation Number
- 36C24E18Q0073
- Archive Date
- 3/19/2018
- Point of Contact
- Jeffrey M Greis
- E-Mail Address
-
822-3297<br
- Small Business Set-Aside
- N/A
- Description
- The Veterans Administration (VA) Service Area Office East (SAO-E) R&D Contracting Office, intends to negotiate on a sole-source basis with Becton, Dickinson and Co., (BD) Biosciences Business Unit, One Becton Drive, Franklin Lakes, NJ 07417, for the procurement of a 646500 BD Influx fluorescence-activated cell sorting system for the VA Bronx Medical Center R&D, 130 Kingsbridge Rd., Bronx, NY 10468. This procurement is being conducted in accordance with FAR 6.302-1(a)(1)&(2)(i)(a) and 6.302-1(b)(1)(i) (only 1 responsible source) because the Government believes that BD is the only manufacturer/seller/installer of this system and that only this system has the performance features clinically acceptable for the intended use by VA scientists in a research laboratory setting. Salient characteristics include: Smaller footprint (45 x48 ); Moveable frame on wheels with HEPA-filtered enclosure; Pair of forward scatter detectors capable of separating polarized fluorescence and scatter signals Configurable, exchangeable fluorescence detectors; Sorts 1,026 ways; Bubble detector in the sample line; Configurable with 7 lasers and a 7 pinhole optical collection system; Features AccuDrop patented technology; Configurable and exchangeable fluorescence detectors; 4 cameras for system alignment and sort monitoring easily seen by the user during sorting: A forward scatter camera for alignment of the forward scatter signals for small particle detection; A camera for monitoring pin hole alignment and maximizing fluorescence signals; A camera for monitoring both deflection of side streams and position of the waste stream; A camera for monitoring droplet formation and break off to maximize sorting stability; 4.5 decade, low-noise logarithmic PMT amplifiers; Special ADC conversion mode to determine background signal; Quick-exchange nozzle assembly and exchangeable fluidics to prevent contamination in PCR reactions; 16 analog preamplifiers and 16 logarithmic amplifiers; 5 microsecond cycle time at 16 parameters input; Real-time cross-talk compensation; Spatial separation of fluorescence signals by pinholes in mirrored surface for ease of instrument alignment; Separate laser excitation paths; Option for fluorescence polarizer; Sensitivity scatter: >0.2 micrometer standard (<0.2 micrometer with optional Small Particle Scatter); Fluorescence: better than 125 MESF FITC; Noise level of non-integrating signal input 2 photons or less; Goniometric nozzle positioner alignment; Spatial filter with pinhole for each channel; Phase check for easy and accurate sort adjustment; Closed work space with spill area, stainless steel, cleanable; Laser in vertical plane can be reconfigured; Detector array coupled to microscope image guarantees resolution and background suppression close to theoretical performance limits; Very short sample path, no dead corners, low dead volume; UV sterilization of work space; High-current photomultipliers for extended linearity; Sort stream observation; Forward scatter measured with PMT; Center pinhole for measuring very weak signals or for special experiments; Sort plates move out of the way and can be cleaned; Internal RS-485 loop for steppers and other peripheral devices; Data bus collates event information in transparent package; includes sort decision; will combine compensated and raw data; Accepts a range of nozzle diameters: 70, 100, 140, and 200um; Additional narrative: The Influx has a maximum processing rate of 180,000 cells per second and the capability of achieving a minimum sort rate of 75,000 cells per second with a purity level of 99% or greater. Seven separate pin hole pathways allowing for the use of at least seven (7) lasers of your choice. Up to 10 lasers may be fitted on the Influx. These pathways are user defined by using a unique optical breadboard allowing the user to easily switch lasers from pathway to pathway to quickly change experimental protocols if needed. Up to 24 parameters, 22 fluorescent parameters and FSC and SSC are available. The Influx system comes with Quick Exchange nozzle assembly which mounts with a single screw. The operator can quickly exchange the available nozzle tips with sizes from 70 to 200 microns. Each laser pathway is provided with a high quality stage assembly which allows for accurate signal alignment from each incoming laser and provides maximum signal resolution for low level fluorescence experiments. The fluidic system uses only three (3) pinch valves, none of which comes in contact with the actual sheath/sample fluids allowing for clean sorting and quick and easy swapping of all fluidic lines. These lines can be sterilized for ultimate cleanliness while sorting. A UV light is present in the sorting chamber to help provide a clean environment for sorting. This notice of intent is not for competitive quotes. The North American Industry Classification System (NAICS) number is 334516, small business size standard of 1,000 employees. This notice is NOT a request for competitive proposals and no solicitation document is available. However, parties may express their interest accompanied by supporting literature by email to Jeff Greis, Contracting Officer, no later than March 19, 12:01 ET. Responses should be sent to jeffrey.greis@va.gov. No telephone responses will be accepted. Information received will be for the purpose of determining whether or not to conduct a competitive procurement or proceed with a sole-source procurement. The determination to conduct a competitive procurement based on responses to this notice is solely within the discretion of the VAPHS Contracting Officer. Please include your socioeconomic status (i.e., VOSB, SDVOSB), DUNS, and FSS Schedule numbers (if applicable) in your responses.
- Web Link
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- Document(s)
- Attachment
- File Name: 36C24E18Q0073 36C24E18Q0073.docx (https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=4145887&FileName=36C24E18Q0073-000.docx)
- Link: https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=4145887&FileName=36C24E18Q0073-000.docx
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- File Name: 36C24E18Q0073 36C24E18Q0073.docx (https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=4145887&FileName=36C24E18Q0073-000.docx)
- Record
- SN04853019-W 20180315/180313231704-61323db6dde682ed5c0e6bfa52840a62 (fbodaily.com)
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