SOLICITATION NOTICE
66 -- Transcriptome Analysis of Pluripotent Stem Cell Differentiation Process at the Single-Cell Level
- Notice Date
- 3/9/2018
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, NCATS, National Institute on Drug Abuse, National Center for Advancing Translational Science (NCATS), 6001 Executive Boulevard, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-CSS-2018-099
- Archive Date
- 3/29/2018
- Point of Contact
- Jessica Adams, Phone: 3044438761, Jeffrey Schmidt,
- E-Mail Address
-
jessica.adams@nih.gov, jeffrey.schmidt@nih.gov
(jessica.adams@nih.gov, jeffrey.schmidt@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Title: Transcriptome Analysis of Pluripotent Stem Cell Differentiation Process at the Single-Cell Level (i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. (ii) The solicitation number is HHS-NIH-NIDA-CSS-2018-099 and the solicitation is issued as a request for quotation (RFQ). This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures; FAR Subpart 13.5-Simplified Procedures for Certain Commercial Items; and FAR Part 12-Acquisition of Commercial Items, and is not expected to exceed the simplified acquisition threshold. THIS IS A NON-COMPETITIVE (NOTICE OF INTENT) COMBINED SYNOPSIS SOLICITATION TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). The National Institute on Drug Abuse (NIDA), Station Support Contracts and Simplified Acquisitions Branch (SS/SA) intends to negotiate and award a purchase order without providing for full and open competition (Including brand-name) to 10X Genomics, Inc. for seven (7) x "Chromium TM Single Cell 3' Library & Gel Bead Kit v2, 16 rxns", three (3) x "Chromium TM Single Cell A Chip Kit, 48 rxns" and two (2) x "Chromium TM i7 Multiplex Kit, 96 rxns" which are required to continue to perform single cell analysis by the Stem Cell Translation Laboratory (SCTL). This acquisition is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures, Subpart 13.106-1 (b) (1), Soliciting from a single source. Pursuant to FAR Subpart 13.501 (a) (1) (iii) the justification (excluding brand name) will be made available within 14 days after contract award or in the case of unusual and compelling urgency within 30 days after contract award. Pursuant to FAR Subpart 13.501 (a) (1) (iv) the rationale for the brand name justification: This particular brand name is needed, to the exclusion of other sources, because the "Chromium TM Single Cell 3' Library & Gel Bead Kit v2, 16 rxns" (reagent kits), "Chromium TM Single Cell A Chip Kit, 48 rxns" (chip kits), and "Chromium TM i7 Multiplex Kit, 96 rxns" (multiplex kits) described are compatible with the 10X Genomics Chromium Single Cell Controller (10X Genomics), an instrument that is currently housed in the SCTL space. The 10X Genomics generates long ranges of information, including high quality sequencing libraries. The sequencing libraries are compatible with Illumina sequencers. No other company offers a single cell preparation platform with the same capacity as 10X Genomics, which can prepare up to 80,000 cells for a single library and 8 libraries in a single run. 10X Genomics also provide open source data analysis tools for analysis pipeline and data visualization (Cell ranger and Loupe cell browser). (iii) The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-96, dated November 6, 2017. (iv) The associated NAICS code 334516 and the small business size standard 1000 employees. This requirement has no set-aside restrictions. (v) The purpose of this acquisition is to acquire the following: 1. Chromium TM Single Cell 3' Library & Gel Bead Kit v2, 16 rxns: • Generates single cell information from tens of thousands of transcripts per cell • Upgrades existing short read sequencers to generate cell by cell 3' mRNA counts for thousands of cells per run. • Produces up to 100K individual nanoliter reactions coupled with ~750,000 unique molecular barcodes • Allows detection of differential gene expression between individual cells. • Provides stand alone and turn-key analysis pipelines and visualization software; open source data analysis tools for rapid processing of Illumina sequencing data • Enables single cell 3' counting of mRNA at 100X reduction in price compared to commonly used single cell platforms • Prepares up to 80,000 cells for single cell library preparation in 7 minutes • Requires low capital outlay • Recovers up to ~65% of cells (typically 50%) • Low doublet rate (~0.9% per 1,000 cells) • Compatible with 10X Genomics Chromium Single Cell Controller • Compatible with Illumina HiSeq4000, NextSeq, and MiSeq sequencers 2. Chromium TM Single Cell A Chip Kit, 48 rxns: • 6 chips, 48 reactions • Loaded with the Gel Beads, 1ng of high molecular weight DNA, and an oilsurfactant solution • Compatible with 10X Genomics Chromium Single Cell Controller 3. Chromium TM i7 Multiplex Kit, 96 rxns • Contains ready-to-use balanced oligonucleotide mix in each well • Compatible with 10X Genomics Chromium Single Cell Controller • Compatible with Illumina HiSeq4000, NextSeq, and MiSeq sequencers (vi) Generic Name of Product: 1. Reagent kits 2. Chip kits 3. Multiplex kits (vii) Delivery: Shipment #1: Three (3) x Chromium TM Single Cell A Chip Kit, 48 rxns, four (4) x Chromium TM Single Cell 3' Library & Gel Bead Kit v2, 16 rxns, and two (2) x Chromium TM i7 Multiplex Kit, 96 rxns. Shipment #1 must be delivered within 2 weeks of initial receipt of order by the vendor. Shipment #2: The remaining three (3) x Chromium TM Single Cell 3' Library & Gel Bead Kit v2, 16 rxns. Shipment #2 must be delivered 4 weeks after Shipment #1 is sent by the vendor. (viii) The provision at FAR clause 52.212-1, Instructions to Offerors - Commercial Items, applies to this acquisition. (ix) The provision at FAR clause 52.212-2, Evaluation - Commercial Items, applies to this acquisition. (a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers: 1. Technical Capability • The essential features list in this solicitation must be provided in entirety. • The material or service must be compatible in all aspects (form, fit, and function) with existing systems presently installed. • The new items are "direct replacement" parts / components for existing equipment. 2. Price • Technical capability is significantly more important than cost. (b) A written notice of award or acceptance of an offer, mailed or otherwise furnished to the successful offeror within the time for acceptance specified in the offer, shall result in a binding contract without further action by either party. Before the offer's specified expiration time, the Government may accept an offer (or part of an offer), whether or not there are negotiations after its receipt, unless a written notice of withdrawal is received before award. (x) The Offerors will include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications-Commercial Items, with its offer. (xi) The FAR clause at 52.212-4, Contract Terms and Conditions - Commercial Items, applies to this acquisition (xii) FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items, applies to this acquisition. (xiii) There are no additional contract requirement(s) or terms and conditions applicable to this acquisition. (xiv) The Defense Priorities and Allocations System (DPAS) are not applicable to this requirement. (xv) Responses to this solicitation must include sufficient information to establish the interested parties' bona-fide capabilities of providing the product or service. The price quote shall include: unit price, list price, shipping and handling costs, delivery days after contract award, delivery terms, prompt payment discount terms, F.O.B. Point (Destination or Origin), product or catalog number(s); product description; and any other information or factors that may be considered in the award decision. Such factors may include: past performance; special features required for effective program performance; trade-in considerations; probable life of the item selected as compared with that of a comparable item; warranty considerations; maintenance availability; and environmental and energy efficiency considerations. Respondents that believe that they are manufacturers or authorized resellers of the brand-name product specified in this announcement must provide, as part of their response: (a) product, catalog, model, and/or part number(s); (b) product description; (c) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in the purchase description; quantity; estimated price or cost; shipping, handling, and/or installation charges; and delivery date after receipt of order. In addition the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." All responses must be received by Wednesday, March 14, 2018, at 1 PM Eastern and reference number HHS-NIH-NIDA-CSS-2018-099. Responses may be submitted electronically to Jessica Adams, jessica.adams@nih.gov. Fax responses will not be accepted. (xvi) The name and telephone number of the individual to contact for information regarding the solicitation: Jessica Adams at (301) 443-8761
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/notices/79f242c0eedd9aebf21d5f4b4df4e673)
- Place of Performance
- Address: Rockville, Maryland, 20850, United States
- Zip Code: 20850
- Zip Code: 20850
- Record
- SN04849060-W 20180311/180309230857-79f242c0eedd9aebf21d5f4b4df4e673 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |