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FBO DAILY - FEDBIZOPPS ISSUE OF MAY 10, 2017 FBO #5647
DOCUMENT

Q -- RNA Sequence Analysis (Research) - Attachment

Notice Date

5/8/2017
 

Notice Type

Attachment
 

NAICS

621511 — Medical Laboratories
 

Contracting Office

Department of Veterans Affairs;Network Contracting Office 2;James J. Peters VA Medical Center;130 West Kingsbridge Road;Bronx NY 10468-3904
 

ZIP Code

10468-3904
 

Solicitation Number

VA24217Q0371
 

Response Due

5/18/2017
 

Archive Date

7/17/2017
 

Point of Contact

Josie Tuitt
 

E-Mail Address

Josie.Tuitt@va.gov
(josie.tuitt@va.gov)
 

Small Business Set-Aside

N/A
 

Description

Network Contracting Office 2 - James J. Peters VA Medical Center Campus is seeking service for RNA (ribonucleic acid) sequencing analysis (Research). RNA-seq is a next-generation sequencing technique that measures the abundance of RNA transcripts in a sample. In a standard RNA-seq procedure, total RNA first goes through a poly-A pull-down for mRNA purification, and then goes through reverse transcription to generate cDNA. The cDNA is broken into a library of small fragments, attached to oligonucleotide adapters that facilitate the sequencing reaction, and then sequenced either single-ended or pair-ended. Finally, the reads are aligned to a reference genome to estimate the expression level of known or novel transcripts. The results can indicate differences in transcriptional structure and/or in expression levels of specific genes. To study noncoding RNAs that lack poly-A tails, the poly-A pull-down step is replaced with a ribosomal RNA reduction experiment. Performance will be at the Contractor s facility with the results sent electronically to the research study investigator at James J. Peters VA Medical Center.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/VA/BroVANAP/VAMD/VA24217Q0371/listing.html)
 

Document(s)

Attachment
 

File Name: VA242-17-Q-0371 VA242-17-Q-0371_1.docx (https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=3479400&FileName=VA242-17-Q-0371-000.docx)

Link: https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=3479400&FileName=VA242-17-Q-0371-000.docx

 

Note: If links are broken, refer to Point of Contact above or contact the FBO Help Desk at 877-472-3779.
 

Place of Performance

Address: James J. Peters VA Medical Center;130 W. Kingsbridge Road;Bronx

Zip Code: 10468
 

Record

SN04499562-W 20170510/170508234510-393b9600319c96f5e065f222d0115b02 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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