SOLICITATION NOTICE
66 -- Immunosenescence and Inflammatin Associated with Aging - Request for Quotation
- Notice Date
- 9/8/2016
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 3155, MSC 9593, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-2016-RFQ-543
- Archive Date
- 9/27/2016
- Point of Contact
- Danielle R. Brown, Phone: 301 480 2385, Rachelle Trice, Phone: 301 594 1928
- E-Mail Address
-
danielle.brown2@nih.gov, rachelle.trice@nih.gov
(danielle.brown2@nih.gov, rachelle.trice@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Solicitation - Request for Quotation The requested procurement order is to contract deep sequencing of RNA and chromatin immunoprecipipitation (chip) cell subsets obtained from human peripheral blood mononuclear cells from volunteers for ongoing projects (BLSA, GESTALT, normal donors). Additionally, deep sequencing will be carried out to measure chromatin accessibility changes with age and chromatin changes conformational changes (ChIA-Pet). The proposed project will examine age-associated changes in gene expression and epigenetic state of 10 cell subsets purified from human peripheral blood. This is a collaborative venture that involves several NIA/IRP laboratories, including, LMBI, TGB, LMG, and LG. Cytapheresis packs will be collected from 30 volunteers of varying age-groups and cells fractioned by a combination of magnetic bead purification and flow cytometry. These cells will be used to isolate RNA and carry out ChIP and ATAC. Purified RNA and DNA will be provided to Dr. Hao for deep sequencing. Additionally, ChIA-Pet will be used to obtain chromatin structural information. ATAC and ChIA-Pet libraries will be prepared at the NIA and provided to the contractor.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/HHS-NIH-NIDA-SSSA-2016-RFQ-543/listing.html)
- Place of Performance
- Address: 733 North Broadway, Room 351, Baltimore, Maryland, 21205, United States
- Zip Code: 21205
- Record
- SN04262456-W 20160910/160908234933-f0d9ee3161cbb8e1cb1264227fbb2ce9 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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