SOLICITATION NOTICE
Q -- Explore the mechanistic link between genome architecture, transcription and recombination in B cells.
- Notice Date
- 9/1/2016
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- HHSN26816AR00018S
- Archive Date
- 9/22/2016
- Point of Contact
- Kyle Wisor, Phone: 3014023670, Lisa Portner, Phone: 3014350326
- E-Mail Address
-
Kyle.Wisor@nih.gov, Lisa.Portner@nih.gov
(Kyle.Wisor@nih.gov, Lisa.Portner@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- (1) Action Code: Combined Synopsis/Solicitation (2) Date: September 1 (3) Year: 2016 (4) Contracting Office Zip Code: 20892 (5) Classification Code: Q301 (6) Contracting Office Address: 6701 Rockledge Drive Rockledge II Bethesda, MD 20892 (7) Subject/Title: Explore the mechanistic link between genome architecture, transcription and recombination in B cells. (8) Proposed Solicitation Number: HHSN26816AR00018S (9) Closing Response Date: September 7, 2016 (10) Contact Point: Kyle Wisor, Contract Specialist (11) Contract Award and Solicitation Number: TBD (12) Contract Award Dollar Amount: UKN (13) Contract Line Item Number(s): See 1449 (14) Contractor Award Date: UKN (15) Contractor Name: TBD (16) Description: The National Institutes of Health (NIH) is the nation's leading medical research agency, and the primary Federal agency conducting and supporting medical discoveries that improve people's health and save lives. The mission of the National Institute of Arthritis and Musculoskeletal and Skin Diseases is to support research into the causes, treatment, and prevention of arthritis and musculoskeletal and skin diseases; the training of basic and clinical scientists to carry out this research; and the dissemination of information on research progress in these diseases. The goal of the Genomics and Immunity Section (GIS) is to unravel the molecular mechanisms driving early development and peripheral activation of B lymphocytes. In particular, the section is interested in the processes that assemble, diversify, and provide effector functions to antibody receptors, namely V(D)J recombination, somatic hypermutation, and class switching. Another major interest of the laboratory is to understand how deregulation of these reactions leads to B cell tumorigenesis. Related to the proposed project, both transcription and recombination of immunoglobulin genes are believed to be regulated by genome architecture. However there is still very little that is known about this topic simply because the techniques to explore architecture have just been developed in the last three years. (i) "This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued." (ii) The solicitation number is HHSN26816AR00018S and the solicitation is issued as a request for quote (RFQ). (iii) The solicitation document, the incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-89 effective 15 AUG 2016. (iv) The associated NAICS code is 541711, Research and Development in Biotechnology, and the small business size standard is 1000 Employees. The acquisition is being conducted in accordance with the procedures of FAR Part 12 and FAR Part 13. (v) The Contractor Requirements are listed below: (vi) The Contractor Shall Provide the following services: Tasks: To generate a comprehensive map of B cell architecture the GIS has established the following protocol: 1. The contractor shall isolate various mouse primary B cell samples: • The contractor shall enrich with magnetic beads different B cell populations, including resting naïve cells from the spleen. Cells will be processed untreated or they will be activated ex-vivo in the presence of mitogens (LSP) and cytokines (IL4). Furthermore, the intent is to process both cells isolated from WT mice or myc deficient ones, since in the absence of myc global transcription is compromised. B cell activation under conditions of ATP depletion or inhibition of histone deacetylation will also explore whether genome architecture requires energy or histone acetylation. 2. The contractor shall perform ten (10) runs, generating deep- sequencing HiC libraries. Perform the high-throughput sequencing using Illumina's X Ten machines from 20 samples provided by the GIS: • These state of the art machines can sequence up to 3 billion sequence reads per run. This is the range that is needed to uncover long-range chromatin interactions in any genome. Detailed HiC maps at his scale have only been produced a few times so far. With the new Illumina machines one can easily achieve the required output. 3. The contractor shall perform bioinformatic analysis: • Produce working maps of genome architecture the contractor's bioinformaticians will align the sequencing data to the mouse genome and transfer the files to NIAMS. Two dedicated bioinformaticians in NIAMS will perform all subsequent analyses to generate figures for the various papers. These last have been using R and other programing software, to write scripts that deal with the high-throughput nature of HiC libraries. Contractor Requirements: 1. The Contractor shall have access to an Illumina 10 X to perform the deep and high-throughput sequencing; 2. Experience with the in situ HiC technique Government Requirements: 1. The GIS will isolate and activate B cells ex-vivo, from wild type and genetically modified mice. In addition, The GIS will study nuclear architecture in gene targeted CH12 B cell lines. Using CRISPR technology, the GIS has generated several point mutations at architectural factors. These mutants will help clarify how these proteins set up genome folding in mammalian cells; 2. Bioinformatic analysis: The GIS has two (2) dedicated bioinformaticians who will analyze the high-throughput data and help write the manuscripts. Deliverables: 1. The contractor will deliver HiC sequences within 3-4 months after contract award; 2. Manuscript completion end of period of performance (vii) Period of Performance: Base Year: September 16, 2016 - September 15, 2017 Option Period 1: September 16, 2017 - March 15, 2017 (viii) The provision at FAR clause 52.212-1, Instructions to Offerors - Commercial Items, applies to this acquisition. (ix) The provision at FAR clause 52.212-2, Evaluation - Commercial Items, is applicable. The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation provides the most adventagous quote to the Government, technical factors considered. Technical Factors • Demonstrated experience with the in situ HiC Technique • Bioinformatician's experience with mapping of genome architecture (x) FAR clause 52.212-3, Offeror Representations and Certifications - Commercial Items, is applicable. An offeror shall complete only paragraphs (b) of this provision if the offeror has completed the annual representations and certificates electronically via http://www.acquisition.gov. If an offeror has not completed the annual representations and certifications electronically at the System for Award Management (SAM) website, the offeror shall complete only paragraphs (c) through (p) of this provision. (xi) FAR clause at 52.212-4, Contract Terms and Conditions - Commercial Items, applies to this acquisition. (xii) FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items, applies to this acquisition. Including the following clauses and Prohibitions: i. 52.222-3, Convict Labor ii. 52.222-21, Prohibition of Segregated Facilities iii. 52.222-26, Equal Opportunity iv. 52.222-36, Equal Opportunity for Workers with Disabilities v. 52.222-50 Combating Trafficking in Persons vi. 52.223-18, Encouraging Contractor Policies to Ban Text Messaging while Driving vii. 52.225-13, Restrictions on Certain Foreign Purchases (xiii) Additional Clauses: i. 52.2232-39, Unenforceability of Unauthorized Obligations ii. 52.227-14, Rights in Data - General iii. 52.217-8, Option to Extend Services--The Government may require continued performance of any services within the limits and at the rates specified in the contract. These rates may be adjusted only as a result of revisions to prevailing labor rates provided by the Secretary of Labor. The option provision may be exercised more than once, but the total extension of performance hereunder shall not exceed 6 months. The Contracting Officer may exercise the option by written notice to the Contractor within 30 days. (xiv) The Defense Priorities and Allocations System (DPAS) and assigned rating are not applicable. (xv) All responses must be received by September 7, 2016 at 9:00 AM EST and must reference number HHSN26816AR00018S. "Offers shall be submitted via the FedConnect web portal (www.fedconnect.net). For more questions please contact the Contract Specialist, Kyle Wisor, Phone: 301-402-3670. (17) Place of Contract Performance: National Institute of Health, NIAMS 10 Center Drive Building 10 Bethesda, MD 20892 (18) Set-aside Status: None Effective on or about March 1, 2016, The National Heart Lung and Blood Institute (NHLBI), Office of Acquisitions (OA) will begin using Compusearch's FedConnect as the communications web portal for all receipt of proposals, unless an exception applies as stated in the solicitation. Therefore potential offerors must be registered in FedConnect in order to submit proposals in response to future R&D, non-R&D Open Market procurements for the NHLBI and for which the NHLBI Consolidated Operations Acquisition Center (COAC) provides acquisition services. FedConnect is a web portal that connects agencies and vendors to streamline the process of doing business with the federal government. It also provides an open channel of communication with the government that is both secure and auditable. Vendors can register with FedConnect at https://www.fedconnect.net/FedConnect/default.htm. Please note that FedConnect is used by multiple federal agencies and therefore FedConnect assistance will be provided by Compusearch Software Systems, not the NHLBI OA. More information about registration requirements can be found by downloading the FedConnect Ready, Set, Go! Guide at https://www.fedconnect.net/fedconnect/Marketing/Documents/FedConnect_Ready_Set_Go.pdf. For assistance in registering or for other FedConnect technical questions please call the FedConnect Help Desk at (800) 899-6665 or email at support@fedconnect.net.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/HHSN26816AR00018S/listing.html)
- Place of Performance
- Address: National Institutes of Health, NIAMS, 10 Center Drive, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN04251403-W 20160903/160901235024-7da1727e690657484ac17d22bfceee1f (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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