SOLICITATION NOTICE
66 -- Total Internal Reflection Fluorescence Microscope System (Inverted) - Copy of the Solicitation
- Notice Date
- 8/22/2016
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-CSS-16-686
- Archive Date
- 9/22/2016
- Point of Contact
- Lauren M. Phelps, Phone: 3015942490
- E-Mail Address
-
lauren.phelps@nih.gov
(lauren.phelps@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Copy of the Solicitation in PDF Format Total Internal Reflection Fluorescence Microscope System (Inverted) Competitive Combined Synopsis/Solicitation HHS-NIH-NIDA-SSSA-CSS-16-686 INTRODUCTION This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. This acquisition is being conducted in accordance with the procedures of FAR Part 13--Simplified Acquisition Procedures, FAR Subpart 13.5--Test Program for Certain Commercial Items, and FAR Part 12--Acquisition of Commercial Items. The solicitation number is HHS-NIH-NIDA-SSSA-CSS-16-686 and the solicitation is issued as a request for quotation (RFQ). NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE The intended procurement is classified under NAICS code 334516 with a Size Standard of 1,000 Employees. SET-ASIDE STATUS This acquisition is unrestricted. Businesses of any size classification may respond. ACQUISITION AUTHORITY This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures, FAR Subpart 13.5--Test Program for Certain Commercial Items, and FAR Part 12-Acquisition of Commercial Items, and IS expected to exceed the simplified acquisition threshold. The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-89, dated July 14, 2016. The resultant contract will include all applicable provisions and clauses in effect through this date. DESCRIPTION OF REQUIREMENT Background The Membrane Transport Biophysics Section (MTBS) of the National Institute of Neurological Disorders and Stroke (NINDS) studies the functions of transporter proteins in cellular membranes and their biological roles. A major focus of MTBS work is the understanding of the role of chloride transport in subcellular organelles, including lysosomes. The Section has identified a chloride transporting protein, ClC-7, which is involved in the mechanisms by which these organelles generate an acidic interior. The Section has been studying the action of ClC-7 using a variety of approaches, including biochemical analysis and microscopy, but for new experimental directions, requires the ability to assess the acidification of individual lysosomes isolated from living cells. To do these experiments requires a specialized microscope to visualize the individual lysosomes using Total Internal Reflection Fluorescence microscopy (TIRF). Purpose The purpose of this requirement is to acquire one (1) Total Internal Reflection Fluorescence microscope system (inverted) to be used to capture high-quality images of live cells and individual isolated lysosomes for studies of lysosomal function and dysfunction. Project Requirements Equipment Specifications The NINDS MTBS requires one (1) fully motorized TIRF microscope system (inverted) that shall have a turn-key solution for hardware-driven sequencing of the activity of excitation lasers, x-y-z axis movements, filter wheels, and cameras. The equipment shall meet the following requirements: A.The system must be based on a fully motorized inverted microscope stand with motorized control of extra-fine/fine/coarse focus, filter cube changing, escape/refocus mechanism, light distribution, and motorized shutters for each light source. The body must also contain an intermediate magnification changer with 1.5x, and left and right sideports for cameras. 10x eyepieces (with adjustable diopter correction) must be mounted on an adjustable eyepiece tube with varying inter-pupilary distance. B.The system must have a 100W halogen lamp with appropriate power supply and condenser turret for transmitted light illumination with an appropriate high transmission daylight colorbalance filter. The condenser should be a long-working distance type with at least 0.52NA. The condenser turret should contain at least 5 positions in which DIC prisms, phase annuli and other transmitted light modules may be housed. C.The system must have 4x, 10x, and 40 x high quality (plan fluor) objective lenses, and anumerical aperture of at least 1.49 and apochromat 60x oil immersion lens with a correction collar for TIRF imaging. All objective lenses must be equipped with appropriate prisms for Differential Interference Contrast imaging. D.The system must have a motorized X-Y scanning stage with linear encoders and an integrated encoded motorized z axis drive. Interactive control of stage axes, filter changers, shutters, focus, and reflector changers is required. E.The system must come with three (3) holders for 55x25 mm labtek chambered coverslips, 35 mm Petri Dishes, or 1x3" microscope slides. F.The system must have an LED-based white-light illumination system for wide-field fluorescence and filter cubes for DAPI, FITC, TRITC, and Cy5. G.The system must include lasers for objective-based TIRF illumination and alaser launch for TIRF. Lasers must include lasers with wavelengths of 445, 488 561 and 640 nm (20 mW each at the microscope) and be capable of supporting up to three more lasers in the future. The laser launch should include high speed laser shuttering and attenuation via an Acousto-Optical Tunable Filter (AOTF). The system should have the ability to switch between full TIRF laser illumination, full epifluorescence illumination and a combined TIRF/epi imaging modality. Dichroic mirrors included in the TIRF package should be configured so that excitation with 445 and 488 lasers can be alternated at maximum speed. H.The system must be able to utilize a Hammamatsu ORCA Flash4.0 v2+ sCMOS camera with USB 3.0 interface to achieve maximum field of view and sensitivity with 82% quantum efficiency. The system must be upgradable to 100 fps imaging and direct hardware triggering acquisition schemes in which the camera synchronizes the timing of the AOTF and any additional analog or TTL controllable devices. I.The system must include a 64bit Imaging workstation computer with Xeon 3.0GHz 8 Core CPU, 64GB DDR4 RAM, a256GB SSD OS hard drive, 4TB (2x2TB striped RAID 0) data storage hard drive, 4GB Video card,optical DVDRW drives, 2 LAN ethernet adapters, USB 3 x8 andx2 USB 2 ports, keyboard and laser optical mouse, and recovery software included. Windows 7 64bit professional edition. The computer system must include a 3 year warranty for parts, labor, and service, and two 24" LED backlit high resolution monitors with display port cables. J.The system must include a real-time Z drift correction system that maintains a fixed focal plane setting in the settings of potential thermal drift and uneven plate surfaces. This drift correction mechanism should actively track the axial location of the sample vessel up to 20 times per second. The drift correction system should utilize an offset lens that allows the IR LED used for drift correction and camera to be focused simultaneously at two different planes of focus, thereby allowing simultaneous imaging and real-time active drift correction. K.The system must include software that enables full computerized control of all microscope features and image acquisition. The software should include abilities for multidimensional acquisition, (including XY, Z, T and large image tiling), real-time deconvolution, ratio imaging module specifically designed for real-time ratiometric imaging and calculating FRET efficiency. The software should also be capable of multi-well dish imaging with integrated hardware based autofocus, on-the-fly segmentation algorithms during imaging routines, and automated selection and high-res z-imaging of sub-regions of a specimen based on segmentation algorithms. The system must have the option of being controlled by both Metamorph software and manufacturer-specific software. L.The system must be able to image cells in pre-specified regions and/or wells of multiwall dishes and perform post-acquisition segmentation analysis and quantification of the resulting images in an automated fashion. M.The system must include a 4' x 5' air isolation table with 4" optical top tapped for ¼-20 screws on 1" centers with 4-post support with gimbal piston isolators and height-adjustable casters N.The system should utilize infinity corrected optics that allow for insertion of additional optical tiers between the objective back aperture and microscope tube lens. Each optical tier should be capable of integrating multiple illuminators for maximum flexibility of excitation wavelength combinations and imaging modalities. This second tier should also be capable of housing an emission filterwheel for rapid single-band filtering to before the tube lens, which will allow the filtered emission light to be sent to either the eyepieces or either of two side camera ports. O.A rear port unit should be available for the system, which allows a camera or excitation illuminator to be mounted for specialized photostimulation or emission splitting applications, such as FRET or ratiometric imaging. P.For the purposes of future expandability, the software must be able to drive two Hamamatsu flash 4.0 V2+ sCMOS cameras in a synchronized dual trigger mode for a minimum of 100fps simultaneous two-color imaging Q.The software included with the microscope system must includ auto-drift correction, supplemental image contrast based auto focusing, remote control over all motorized components of the microscope system, ratio-metric imaging and ratio calculation, full 3D volume rendering, time measurement for live and post-acquisition recording of image and ROI-based intensity analysis and live ratio imaging, binary operation functions including image segmentation and pixel classification, automated binary and ROI measurement functions, multidimensional acquisition for freely combining multiposition, timelapse Z stacking and multicolor imaging with a high degree of flexibility, and a diverse and accessible macro control language for programming custom functions into acquisition routines. Equipment Warranty Requirements The Contractor shall provide at a minimum, a one (1) year warranty on all equipment, supplies and repairs. Contractor shall provide technical support service during this period. Technical support service shall be on-site and/or virtual and include technical assistance and training on all service related issues. All products shall be warranted to be free from material defects in workmanship or material under normal use and service for warranty period. Training Requirements On-site training must take place at the customer site within two (2) weeks of equipment installation. Training instruct users on the operation of the instrument and shall be for up to five (5) individuals over a two (2) day period for duration of at least eight (8) hours per day. Training shall take place between the hours of 8:00am and 5:00pm, Bethesda, MD local prevailing time, Monday through Friday. Delivery Requirements/Period of Performance The anticipated contract period of performance is fourteen (14) weeks from date of award. The contractor shall deliver and install the required equipment within twelve (12) weeks after receipt of order. Delivery must be FOB Destination and must include inside installation. The equipment shall be delivered and installed between the hours of 8:00am and 5:00pm, Bethesda, MD local prevailing time, Monday through Friday. Onsite training must take place within two (2) weeks following installation. The contractor shall provide a company certified service technician to perform setup, installation and training. Place of Performance The required equipment shall be delivered and installed inside at the NIH Main Campus, Building 35, Room 3D, located at 35 Convent Drive, Bethesda, MD 20892. Contract Type The Government intends to issue a firm fixed price commercial items contract for this requirement. Question and Answer Period Interested contractors may submit questions relating to this requirement. Questions shall be submitted to the contract specialist, Lauren Phelps, via email at lauren.phelps@nih.gov by or before August 25, 2016 at 5:00 PM EST. Late questions shall not be accepted. Questions will be anonymized and answered and the answers will be provided as soon as possible after the question deadline via solicitation amendment. Should no questions be received, no amendment shall be posted. RESPONSE FORMAT Responses to this solicitation must include clear and convincing evidence of the offeror's capability of fulfilling the requirement as it relates to the technical evaluation criteria. The offeror must submit 1) a technical proposal and 2) a separate price proposal. The technical proposal should be prepared in reference to each of the evaluation criteria identified in this solicitation and may be up to twenty-five (25) single-sided pages. The price proposal must include the requirements listed above as well as associated pricing. Contractors must provide their Company Name, Dun & Bradstreet Number (DUNS), Taxpayer Identification Number (TIN), Business Size, Physical Address, and Point of Contact Information in their responses. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." EVALUATION CRITERIA FAR clause 52.212-2, Evaluation - Commercial Items applies to this acquisition and the specific evaluation criteria to be included in paragraph (a) of that provision are as follows: The Government will award a contract resulting from this solicitation on the basis of best value, technical factors and price considered. Technical factors together shall be considered more important than price. The technical evaluation factors are as follows, in order of importance: 1)Factor 1: Equipment Capability The contractor shall detail in its technical proposal how it shall meet the requirements as set forth in this Statement of Work. Proposal shall include detailed specifications of the offered equipment and its capabilities. The Government shall particularly evaluated for system capability of integrating 3rd party hardware devices in the future if needed. 2)Factor 2: Equipment Warranty The contractor shall detail in its technical proposal the terms and conditions for its proposed warranty. The proposed approach shall be evaluated for the ways in which it meets or exceeds the equipment warranty requirements. 3)Factor 3: Delivery, Installation, and Training The contractor shall detail in its technical proposal how it shall meet the delivery, installation, and training requirements. The Government shall evaluate for ability to meet or exceed these requirements. Proposals must include a delivery lead-time and explanation of who shall perform installation, the installation time frame, as well as what training will be provided and how it shall be delivered. 4)Factor 4: Past Performance The Contractor shall provide a list of two (2) contracts or purchase orders completed during the past five (5) years, similar in size and scope to the microscope requirements outlined in this Statement of Work. Experience must be specific to provision of TIRF microscopes and shall include the following information for each contract or purchase order listed: a.Name of Contracting Organization b.Contract Number c.Total Contract Value d.Description of Requirement as it Related to this SOW e.Contract Period of Performance Past Performance shall be evaluated for relevance to the current requirement. APPLICABLE CLAUSES AND PROVISIONS The following FAR clauses and provisions shall apply to this solicitation: 1.All Offerors MUST be actively registered in the System for Award Management (SAM) www.sam.gov. 2.The provision at FAR clause 52.212-1, Instructions to Offerors - Commercial Items (April 2014), applies to this acquisition. 3.FAR clause 52.212-2, Evaluation - Commercial Items (October 2014) applies to this acquisition. 4.A completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications - Commercial Items (March 2015), is required with any offer submitted. This requirement may be met by completion of the provision in the System for Award Management. 5.FAR clauses 52.212-4, Contract Terms and Conditions - Commercial Items (May 2015) and 52.212-5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items (May 2015) apply to this acquisition. 6. FAR Clause 52.237-2 -- Protection of Government Buildings, Equipment, and Vegetation applies to this acquisition. 7.The Defense Priorities and Allocations System (DPAS) are not applicable to this requirement. In addition, the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All Offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." CONTRACTOR PERFORMANCE EVALUATION Final Evaluation of Contractor performance will be prepared for the resulting contract in accordance with FAR Subpart 42.15. The final performance evaluation will be prepared at the time of completion of work. Final evaluation will be provided to the Contractor as soon as practicable after completion of the evaluation. The Contractor will be permitted thirty days to review the document and to submit additional information or a rebutting statement. If agreement cannot be reached between the parties, the matter will be referred to an individual one level above the Contracting Officer, whose decision will be final. Copies of the evaluation, Contractor responses, and review comments, if any, will be retained as part of the contract file, and may be used to support future award decisions. Contractors may access evaluations through a secure Web site for review and comment at the following address: http://www.cpars.gov CONTRACTING OFFICER'S REPRESENTATIVE A Contracting Officer's Representative (COR) shall be assigned to the awarded contract. The COR is responsible for: (1) monitoring the Contractor's technical progress, including the surveillance and assessment of performance and recommending to the Contracting Officer changes in requirements; (2) interpreting the statement of work and any other technical performance requirements; (3) performing technical evaluation as required; (4) performing technical inspections and acceptances required by this contract; and (5) assisting in the resolution of technical problems encountered during performance. The Contracting Officer is the only person with authority to act as agent of the Government under this contract. Only the Contracting Officer has authority to: (1) direct or negotiate any changes in the statement of work; (2) modify or extend the period of performance; (3) change the delivery schedule; (4) authorize reimbursement to the Contractor for any costs incurred during the performance of this contract; (5) otherwise change any terms and conditions of this contract; or (6) sign written licensing agreements. Any signed agreement shall be incorporated by reference in Section K of the contract. The Government may unilaterally change the COR designation for this contract. CLOSING INFORMATION Responses to this solicitation must include sufficient information to establish the interested parties' bona-fide capabilities of providing the product or service. The price quote shall include: unit price, list price, shipping and handling costs, delivery days after contract award, delivery terms, prompt payment discount terms, F.O.B. Point (Destination or Origin), product or catalog number(s); product description; and any other information or factors that may be considered in the award decision. All responses must be received by the closing date of this announcement and must reference solicitation number HHS-NIH-NIDA-SSSA-CSS-16-686. Responses shall be submitted electronically via email to Lauren Phelps, Contract Specialist, at lauren.phelps@nih.gov.
- Web Link
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FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-2/HHS-NIH-NIDA-SSSA-CSS-16-686/listing.html)
- Place of Performance
- Address: Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN04234250-W 20160824/160822234807-49468c54e1a19069fc6afb1553d2db58 (fbodaily.com)
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