SOLICITATION NOTICE
66 -- Procurement of AKTA Florescent system
- Notice Date
- 7/15/2016
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Bldg 1050, Frederick, Maryland, 21702, United States
- ZIP Code
- 21702
- Solicitation Number
- N02CR62622-57
- Point of Contact
- Reyes Rodriguez, Phone: 240-276-5442
- E-Mail Address
-
reyes.rodriguez@nih.gov
(reyes.rodriguez@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Contracting Office Address Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E128, Bethesda, MD 20892, UNITED STATES Description The National Cancer Institute (NCI), Center for Cancer Research (CCR), Lab of Cell Biology (LCB) plans to procure on a sole source basis one High pressure liquid chromotagraphy HPLC Fluorescence system from GE Healthcare Bio-Science Corp, 100 Results Way, Marlborough, MA 01752. This acquisition will be processed under FAR Part 12 - Acquisition for Commercial Items and will be made pursuant to the authority in FAR 13.106-1 (b)(2) and 13.501-(a)(1) using simplified acquisition procedures for commercial acquisitions. The North American Industry Classification System code is 334516 and the business size standard is 500 employees. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. The estimated delivery date shall be 4-6 weeks ARO. It has been determined there are no opportunities to acquire green products or services for this procurement. Multiple laboratories within the Laboratory of Cell Biology (LCB) are engaged in research that requires the purification of a variety of proteins from crude cellular extracts. Functional studies of many of the proteins require the native proteins in their natural state without affinity tags and therefore conventional chromatography systems are essential to allow isolation of sufficient quantities of purified proteins. The current chromatography systems are shared among four different PIs and are no longer adequate for LCB's needs, which have had a serious negative impact on the rate of research progress. Highly purified proteins are essential reagents in the research program of the majority of PIs in the LCB. Proteins and biologically interesting mutants of the proteins are purified for enzymatic studies, X-ray crystallography and other structural studies, antibody production, and macromolecular interaction studies. Examples of proteins currently being investigated are the multidrug efflux pumps such as human MDR, the oncogenic WIP1 phosphoprotein phosphatase, the ubiquitin-binding molecular chaperone p97 (VCP), and the ATP-dependent proteases ClpXP and ClpAP along with their various adaptors. Contractor Requirements must include: • A modular system design with a large range of options to allow flexibility in purification of proteins and peptides. • Must be practical compact size for easy placement on laboratory bench with a small footprint 535X 630x 470 mm or in cold cabinet. • System must be reliable with components and integrated features based on the proven design of ÄKTA protein purification systems • System must be intuitive and have a flexible method creation, system control, and evaluation with UNICORN software Life Sciences laboratory-scale chromatography columns • A modular system designed for expansion to increase system capability, including the addition of the Loop Valve and Versatile Valve, which allows for automation of multi-step purification schemes • Choice of 15+ available valves to customize the inlet and sample path, column path, and outlet path to meet a wide range of applications • System must have integrated air sensors on selected inlet valves and integrated pre- and post-column pressure sensors on optional Column Selection Valve to protect columns • System must have the ability to connect and control two external fraction collectors to increase capacity • Must include choice of two fraction collector designs based on formats and capacity; fractions can be collected based on time, volume or automatic peak recognition • UNICORN TM 6 software, which provides easy protocol transfer during scale-up and an improved visual interface for intuitive and flexible method editing • Must have the ability to connect two I/O-box E9 units to receive analog or digital signals from external interfacing equipment such as detectors; compatible with Alias TM auto sampler. System salient characteristics include: • Control system Unicorn, version 6.3 or later • Dimensions (W × H × D) 535X 630x 470mm • Weight (excluding computer, sample pump, fraction collector) 53 Kg • Power supply 100-240V, 50-60 Hz • Power consumption 300 VA typical, 25VA power source • Enclosure protective class IP21 System pump • Pump Type Piston pump, metering type • Flow rate setting ÄKTA pure 25: 0.001 to 25 ml/min (up to 50 ml/min during column packing) • Flow rate AKTA pure 25: Accuracy 1-2% Precision: RSD < 0.5% (conditions: 0.25 to 25 ml/min, < 3 MPa, 0.8 to 2 cP) • Pressure range 0 to 20MPa Viscosity range 0.35 to 10cp Sample pump • Pump type Piston pump, metering type Dimensions (W × H × D) Weight 11kg Flow rate setting 0.001 to 50ml/min Flow rate specifications Accuracy +- 2% Precision: RSD less than 0.5% (conditions: 0.25 to 50mlmin, less than 3MPa, 0.8 to 3 cP • Pressure range 0 to 10mPa Viscosity range 0.7 to 10cP Mixer • Mixing principle • Mixer volume • Gradient ow rate range • Gradient composition accuracy Software • UNICORN, version 6.3 or later 535 × 630 × 470 mm Up to 53 kg • 100-240 V, ~50-60 Hz • 300 VA (typical), 25 VA (power-save) IP 21 UV monitoring • Must be equipped with either a variable multiwavelength UV or visible spectrum monitor. • The fixed wavelength (280 nm) UV monitor (U9-L) must incorporate LED technology. • UV monitor U9-L shall not heat the sample. • The monitor must be available with a 2 mm flow cell for higher sensitivity measurements, which are required. • Monitor must determine protein separation at different wavelengths. • UV monitor U9-M must be designed for multiwavelength detection in the UV and visible spectrum from 190 to 700 nm. • UV monitor U9-M must monitor up to three wavelengths simultaneously for optimization performance when purifying samples with different protein concentrations. Three flow cell path lengths available; 0.5, 2, and 10 mm. • Flow cell design and fiber optic technology yield a high signal-to-noise ratio without causing any local heating of the UV flow cell. • Monitor shall contain a high-intensity xenon lamp with a long lifetime that require minimal start-up time. Every time the instrument is switched on, the monitor shall automatically calibrate. The UV signal shall automatically normalize. Additional salient characteristics include: • The NCI work requires the ability to purify in large quantity and high purity biomolecules from complex starting materials derived from cell lysates, tissue homogenates, recombinant protein expression systems and other sources. The purification system that the Laboratory of Cell Biology (LCB) will use must be able to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, hydrophobicity, while retaining biological activity of the biomolecules. • This system shall allow the lab user to easily organize or add system components as needed and customize options available on the system at anytime during the life of the instrument without the need to purchase another platform. • The chromatography system shall have a pumping system, sample and column valves, fraction collector, and columns that run under a single computer controlled operating system. Firmware and software to control the instrument, set user parameters, and collect data installed on a notebook computer with the requisite operating system, networking capability, and storage capacity to run chromatographic separations needed for NCI's research. • In order to protect the existing columns, the system must have a definable pressure limit system, customizable for different types of columns, which will stop the pump and generate an audible and visual warning, in the event pressure exceeds the preset limit. • The chromatography system shall allow the user the option of including in his/her chromatographic methods programs, commands which will automatically reduce the flow rate if an over-pressurization occurs as sample is being applied or eluted from the column. Additional variables which shall also be programmable to trigger sub-routines (e.g. new tube on fraction collector; valve changes; etc.) shall include UV or conductivity signals. • The chromatography system shall permit automatic running of up to 32 different chromatographic programs in a user-designated sequence; it must turn off the pump flow when a signal is received from the fraction collector indicating there are no tubes remaining, so that valuable sample is preserved; the system must automatically and continuously record throughout each chromatographic run the following parameters: flow rate; auto-scaled UV signal; actual AND programmed conductivity of the eluent; eluent pH; system pressure; accumulated time of run; all valve positions and changes in position; activity of a fraction collector - and it must allow these recorded data to be called up later for interpretation and to aid in future replication of results; it must allow for post-run analysis of chromatograms, export of data to other spreadsheet programs, and generation of written reports. • The system must be able to operate remotely. • The system must also be capable of read and manipulate all past chromatographic data for continuity of our research. It should be compact in size, allow the use of current columns for cost savings. • Ability to connect two I/O-box E9 units to receive analog or digital signals from external interfacing equipment such as detectors; compatible with Alias TM autosampler. • Installation and training must be provided by the vendor, at no additional cost. The system quoted shall include spare parts that the lab is likely to need during the first 12-months of operation. • Estimated shipping costs must be included in the quote. • Must allow trade-in of existing equipment AKTA FPLC; model No. UPC900; S/N 18111867-001172. There are two older AKTA systems in the laboratory with components that are compatible with the new system being requested. Purchase of this system allows a seamless transition to use of this upgraded and more stable instrument and is more efficient because it avoids the downtime associated with using new instruments from other manufacturers. In addition, costly columns and column connectors, valves, and tools already in the lab are compatible with and will be used with the new system. The GE AKTA pure 25M HPLC is the only known multimodal chromatography system that can meet the Government's needs at this time. This notice is not a request for competitive quotation. However, if any interested party, especially small business believes it can meet the above requirement, it may submit a proposal or quote for the Government to consider. The response and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement. Responses must be received in the contracting office by 11 AM EST, on July 25, 2016. All responses and questions must be in writing and faxed to 240-276-5399 or emailed to Reyes Rodriguez, Contracting Specialist via electronic mail at reyes.rodriguez@nih.gov. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractors must be registered and have valid certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Applications (ORCA) through sam.gov. Reference: N02CR62622-57 on all correspondence.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/FCRF/N02CR62622-57/listing.html)
- Place of Performance
- Address: TBA, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN04184611-W 20160717/160715234614-215fbcb97d8aef7c1487f678bd892588 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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