SOURCES SOUGHT
65 -- Brand name or equal EVO EMCCD 512 DELTA MFW-16-AC cameras upgrade for SM-FRET
- Notice Date
- 6/7/2016
- Notice Type
- Sources Sought
- NAICS
- 423410
— Photographic Equipment and Supplies Merchant Wholesalers
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E128, Rockville, Maryland, 20852, United States
- ZIP Code
- 20852
- Solicitation Number
- SBSS-N02RC62591-24
- Archive Date
- 6/28/2016
- Point of Contact
- Kimesha Leake, Phone: 2402765669, Jolomi Omatete, Phone: 2402766561
- E-Mail Address
-
kimesha.leake@nih.gov, jolomi.omatete@nih.gov
(kimesha.leake@nih.gov, jolomi.omatete@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Key Dates: Capability Statement Due Date: June 13, 2016 by 10:00 am Eastern This Small Business Sources Sought Notice (SBSS) is for information and planning purposes only and shall not be construed as a solicitation or as an obligation on the part of the National Cancer Institute (NCI). The purpose of this Sources Sought Notice is to identify qualified Small Business concerns including 8(a), HUBZone or Service-Disabled Veteran-owned businesses that are interested in and capable of performing the work described herein. The NCI does not intend to award a contract on the basis of responses received nor otherwise pay for the preparation of any information submitted. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This requirement is assigned North American Industry Classification System (NAICS) code 423410 with a size standard of is 100 employees. As a result of this Sources Sought Notice, the NCI may issue a Request for Quotation (RFQ). THERE IS NO SOLICITATION AVAILABLE AT THIS TIME. However, should such a requirement materialize, no basis for claims against NCI shall arise as a result of a response to this Sources Sought Notice or the NCI's use of such information as either part of our evaluation process or in developing specifications for any subsequent requirement. The Laboratory of Receptor Biology and Gene Expression (LRBGE) is interested in the dynamics of rapid exchange of the transcriptional factors (TF) on promoters. Specifically, LRBGE is interested in extracting biophysical information about sequential interactions for nuclear receptors and other TF on their DNA targets. Pioneering studies from this lab revealed that the interactions of TF with their specific chromatin targets are transient. These observations contradict the classic view of long-term residency of TF; they shed a new light on gene expression mechanisms and open new venues for cancer drug development. A high impact manuscript in press calls into question long held views regarding the mechanisms of transcription factor interaction with chromatin, and demonstrates the continued leadership of the LRBGE in this field. Real-time study of initiation complex assembly and dynamics requires observation of individual molecules in live cells by means of fluorescent microscopy. For these experiments LRBGE custom-built a SMT microscope and developed Matlab-based software for SMT data analysis. LRBGE obtained valuable results, the importance of which was validated by publications in high-profile scientific journals. Further progress requires biophysical characterization of TF interactions. LRBGE needs to register a Forster Resonance Energy Transfer (FRET) effect to prove that the molecules are within 10 nm and thus interact at the same site. However, the current SMT system does not provide adequate excitation, signal detection and simultaneous multicolor imaging for a long-term observation of SM-FRET in dynamics. Our goals will be most adequately met by upgrading the current custom-built system with two (2) high-sensitivity, state-of-the-art EM-CCD. We will use one camera to image the counter-stained nuclei, and a second one for SM-FRET The following salient characteristics apply to the Evo-512-Delta EMCCD Camera: • Frame-transfer EMCCD • Back-thinned EMCCD sensor with electron multiplication to minimize red noise • 512 x 512 array (16x16 um pixels) • High speed triggering • Multiple read-out speeds (67 fps at 20MHz, 1099 fps at 16x16 pix ROI) • Very fast calibration (Rapid-Cal feature) • Highly stabilized cooling performance, Cooling temperature (-75C), • Low dark current (0.003e-/pixel/sec) • 16 bit digitization • TURBO-1394 interface (IEEE-1394a) • Intelligent FGA design • Smart Streaming • PAR feedback system • ACE technology • 20 MHz to 1 mHZ readout • Read noise in EM port (20 MHz, 75e-: 10 MHz, 55e-) • Read noise in non-EM port (5 Mhz, 18e-; 0.625 Mhz, 6e-) • Bias stability < 0.001 ADU/Frame • Extremely stable EM Gain performance; Gain stability (0.0012) • Field uniformity (<1.10) • Linearity >99% • Compatibility with the PVCAM software • Two year warranty The camera must be compatible with the existing custom-built single molecule microscopy system. The current system is based on Photometrics camera, and so the custom designed software that collects the single molecule data communicates with the current camera using the Photometrics PVCAM software. This software is required to read the camera output, and also produce a trigger input to allow synchronization with the lasers that provide the illumination of the specimens. The new camera should use the same PVCAM software to permit a seamless integration with the current system How to Submit a Response: 1. Page Limitations: Interested qualified small business organizations should submit a tailored capability statement for this requirement not to exceed 10 single sided pages including all attachments, resumes, charts, etc. (single spaced, 12 point font minimum) that clearly details the ability to perform the requirements of the notice described above. All proprietary information should be marked as such. Statements should also include an indication of current small business status; this indication should be clearly marked on the first page of your capability statement (preferable placed under the eligible small business concern's name and address). Responses will be reviewed only by NIH personnel and will be held in a confidential manner. 2. Due Date: Capability statements are due no later than June 13, 2016 by 10:00 am Eastern 3. Delivery Point: All information furnished must be in writing and must contain sufficient detail to allow the NCI to determine if it can meet the unique specifications described herein. All questions must be in writing and emailed to Kimesha.leake@nih.gov. A determination by the Government not to compete this requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award, contractors must have valid registration and certification in the System for Award Management (SAM) and the Online Representations and Certifications Applications (ORCA) through sam.gov. No collect calls will be accepted. Please reference number SBSS-N02RC62591-24 on all correspondence. Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a RFQ may be published in Fed Biz Opps. However, responses to this notice will not be considered adequate responses to a solicitation(s). Point of Contact: Inquiries concerning this Notice may be direct to: Kimesha Leake at Kimesha.leake@nih.gov. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/RCB/SBSS-N02RC62591-24/listing.html)
- Record
- SN04140768-W 20160609/160607234730-f9284ca839cb1aa84ab4444976ef960a (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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