SOLICITATION NOTICE
66 -- TrueSeq Stranded Total RNA LT - rfq-64710
- Notice Date
- 2/8/2016
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334513
— Instruments and Related Products Manufacturing for Measuring, Displaying, and Controlling Industrial Process Variables
- Contracting Office
- Department of Health and Human Services, Centers for Disease Control and Prevention, Acquisition and Assistance Field Branch (Hyattsville), 3311 Toledo Road, Room 5303, Hyattsville, Maryland, 20782-2003
- ZIP Code
- 20782-2003
- Solicitation Number
- 2016-q-64710
- Archive Date
- 3/8/2016
- Point of Contact
- Desiree C. Harris, Phone: 3014584629
- E-Mail Address
-
huq9@cdc.gov
(huq9@cdc.gov)
- Small Business Set-Aside
- N/A
- Description
- RFQ The Centers for Disease Control and Prevention intends to award a sole source firm fixed price purchase order to Illumina, Inc., 5200 Illumina Way, San Diego, CA 92122 for lab supplies of TruSeq Stranded Total RNA LT( full listing will be attached). This requirement is determine to be sole source as these supplies are required to global gene (mRNA and miRNA) expression profile by next generation sequencing (NGS) in biological samples such as rat blood and lungs. NGS is the best technique currently available to determine global gene expression profile in biological specimens including blood samples. Depletion of globin mRNA from total RNA is an absolute requirement to obtain accurate global gene expression profile in blood samples by NGS. The TruSeq Stranded Total RNA LT - (with Ribo-Zero Globin) - Set A and Set B are specifically designed by Illumina, Inc, to deplete globin mRNA from blood total RNA while preparing the libraries required for NGS. TruSeq Stranded Total RNA LT - (with Ribo-Zero Globin) - Set A and Set B, sold by the vendor (Illumina, Inc,), is the only commercially available product with capability to simultaneously deplete globin mRNA while preparing sequencing library. Other commercially available kits can either deplete globin mRNA from RNA samples or prepare the sequencing library - not both. Performing globin mRNA depletion and library preparation using separate kits is more time consuming and expensive. This will also require more quantity of blood samples to obtain the quantity of RNA required to deplete globin mRNA and prepare sequencing libraries for NGS. Since blood yields only limited quantity of globin-free RNA, it is important to use a kit that requires the least quantity of RNA to prepare libraries required for global gene expression profiling by NGS. Using kits other than those requested in this purchase order will require using substantially more quantity of blood samples to isolate the amount of RNA to prepare sequencing libraries and this, in turn, will require the use of more number of animals in our experiments. The TruSeq Stranded Total RNA LT - (with Ribo-Zero Gold) - Set A and Set B are required to determine global gene expression profile of lung samples. Using kits from the same vendor (Illumina, Inc) will facilitate accurate comparison of the data obtained from the blood and lung samples of the experimental animals used in the same study. This will also make interpretation of the findings scientifically more valid. The sequencing libraries prepared will be sent to the CDC Genomics Core Facility to determine global gene expression profile by NGS. The CDC Core Facility uses an Illumina Hi-Seq machine to sequence samples. The TrueSeq SBS Kit v3 - HS (50 cycles) and TruSeq PE Cluster Kit v3 - cBot -HS are the only reagents that are compatible with the Hi-Seq sequencing machine to determine global gene expression profile by NGS. This contract action is for supplies or services for which the Government intends to solicit and negotiate with only one source under the authority of FAR 13.106-1(b) (SAP) and 10 U.S.C. 2304(c)(1). Interested persons may identify their interest and capability to respond to the requirement or submit proposals. This notice of intent is not a request for competitive quotations; however, all quotations/responses received within fifteen (15) days of the issuance of this notice will be considered by the government. A determination by the government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. Any quotation/response should be emailed to Desiree Harris; contract specialist at huq9@cdc.gov by COB February 22, 2016 @ 3:00pm EST.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/CDCP/PABW/2016-q-64710/listing.html)
- Place of Performance
- Address: 1095 Willowdale Road, morgantown, West Virginia, 26505, United States
- Zip Code: 26505
- Zip Code: 26505
- Record
- SN04013599-W 20160210/160208234801-7840d433ec999cbdf3d7d13b8310f8b6 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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