SOLICITATION NOTICE
B -- ASSESSMENT OF INTERNAL DOSE/ BIOAVAILABILITY IN CONJUNCTION WITH MEDIUM THROUGHPUT HIGH CONTENT IN VIVO ASSAYS IN ZEBRAFISH - RFQ, NIHES2015062 Assessment Zebrafish
- Notice Date
- 9/16/2015
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute of Environmental Health Sciences, Office of Acquisitions, Office of Management, 530 Davis Drive, Durham, North Carolina, 27713, United States
- ZIP Code
- 27713
- Solicitation Number
- NIHES2016062
- Point of Contact
- Onyenauché Washington, Phone: (919) 541-0423
- E-Mail Address
-
uche.washington@nih.gov
(uche.washington@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- SOW, NIHES2015062 RFQ, NIHES2015062 Assessment Zebrafish This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 in conjunction with FAR 13, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. (1) This solicitation document, NIHES2015063, is issued as a Request for Quotation (RFQ) to provide screening for cardiotoxicity and neurotoxicity combined with heptatotoxicity in zebrafish for the National Institutes of Environmental Health Sciences (NIEHS), Research Triangle Park, NC. (2) This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Regulation (FAR); HHS Acquisition Regulation (HHSAR); Federal Acquisition Circular (FAC) 2005-82 effective May 7, 2015. (3) Notice of Set-Aside, Associated NAICS Code, and Size Standard: This solicitation competed on a full and open competitive basis. The associated NAICS code is 541380, "Testing Laboratories" with a size standard of $15.0M. (4) List of Contract Line Item Number(s) and items, quantities and units of measure, (including option(s): See document titled RFQ, NIHES2015062, Assessment of Internal Dose/ Bioavailability in Conjunction with Medium Throughput High Content in Vivo Assays in Zebrafish. (5) Description of Requirements for the Items to be acquired: The National Institute of Environmental Health Sciences (NIEHS), located in the Research Triangle Park, North Carolina, is one (1) of the 27 Institutes and Centers composing the National Institutes of Health. The NIEHS is home to the National Toxicology Program (NTP), a Health & Human Services (HHS) interagency program dedicated to testing and evaluating substances in our environment. The National Toxicology Program Laboratory (NTPL) exists within the Division of the NTP to provide state-of-the-art laboratory research support that complements and extends the NTP's scientific capabilities and contributes to the identification and understanding of potential health related issues related to environmental/chemical exposures. The zebrafish is an important complementary tool to mammalian toxicity since it can be used to screen a larger number of compounds more rapidly and provide information on prioritizing compounds for subsequent mammalian toxicity studies. There are several ongoing efforts at the NTP exploring the utility of zebrafish as a potential model to screen compounds to prioritize for further mammalian testing. One critical consideration in determining relative toxicity of compounds is their ability to enter the fish. To date, there are no projects that evaluate the bioavailability or internal concentration in zebrafish. Another important requirement is the assessment of ototoxicity following developmental exposure, an important target organ that is currently not being evaluated in other studies. To address this, the NIEHS has requires a Contractor that can multiplex assays to assess ototoxicity and developmental defects and evaluate bioavailability using the HPLC-MS-MS method in a GLP-environment. These studies will provide important complementary information to other ongoing zebrafish efforts to enable prioritization of compounds for further mammalian testing that are currently not being covered. The Contractor shall perform the assessment of bioavailability following exposure to 30 compounds with potential for developmental toxicity in zebrafish embryos. The Contractor shall provide high content screening information on multiple target organs under the same roof using a GLP-environment. Ongoing projects the NTP focus on assessing teratogenicity and molecular end-points in zebrafish following early in life exposure. However, one (1) critical piece of information key to this effort is determining how much of the chemical enters inside the zebrafish. To date the NIEHS does not have a mechanism to assess bioavailability/ internal dosing. The Contractor shall multiplex ototoxicity and developmental defects to be able to design more efficient experiments using fewer fish, to reduce time. The Contractor shall be capable of providing zebrafish based services in the preclinical area, mainly toxicology through early detection of adverse effects in a GLP-setting. The Contractor shall assesse bioavailability /internal dosing following chemical exposure. The Contractor shall perform the assessment of internal dose/ bioavailability in conjunction with medium throughput high content in vivo assays in zebrafish to evaluate developmental effects, ototoxicity and internal concentrations in 30 compounds over five (5) concentrations. Two (2) independent toxicity assays to screen for developmental toxicity and ototoxicity will be carried out at the concentrations selected after the Maximum Tolerated Concentration (MTC) is developed. Once assays are complete, the embryos shall be frozen and will be used to test internal exposure in the concentrations tested by HPLC-Ms-Ms. The widely used AB* zebrafish strain is proposed for use in these studies. (6) Date(s) and place(s) of performance and acceptance and FOB point: A base of 12 months with one (1) 1-year option period. Studies shall be completed within 75 days following receipt of compounds. The opportunities for sample degradation and compromising the quality of data are greater with the continued movement of samples to various sites for analysis, therefore; analysis shall take place in a single facility located within the contiguous United States (CONUS). (7) The provision at FAR 52.212-1, Instructions to Offerors -- Commercial, applies to this acquisition. See document titled RFQ, NIHES2015062, Assessment of Internal Dose/ Bioavailability in Conjunction with Medium Throughput High Content in Vivo Assays in Zebrafish. (8) Applicability of the provision at FAR 52.212-2, Evaluation -- Commercial Items. See document titled RFQ, NIHES2015062, Assessment of Internal Dose/ Bioavailability in Conjunction with Medium Throughput High Content in Vivo Assays in Zebrafish. (9) FAR 52.212-3, applies to this acquisition: Representation, Certifications, and Other Statements of Offerors - In accordance with provisions FAR 52.212-3, Offeror Representations and Certifications--Commercial Items, IF YOU INTEND TO SUBMIT A PROPOSAL, YOU MUST: (1) Go to the Online Representations and Certifications Application (ORCA) and complete the Representations and Certifications. Offerors must access ORCA through the System for Award Management (SAM) at http:// www.sam.gov; and (2). Complete, and INCLUDE as part of your price proposal. (10) FAR 52.212-4 Contract Terms and Conditions Commercial Items, applies to this acquisition. See document titled RFQ, NIHES2015062, Assessment of Internal Dose/ Bioavailability in Conjunction with Medium Throughput High Content in Vivo Assays in Zebrafish. (11) FAR 52.212-5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders--Commercial Items. See document titled RFQ, NIHES2015062, Assessment of Internal Dose/ Bioavailability in Conjunction with Medium Throughput High Content in Vivo Assays in Zebrafish. (12) The Date, Time and Place Offers are Due: The Quoter shall submit dated quote to the following e-mail address: uche.washington@nih.gov at or before Monday, 21 September 2015 at 12:00 PM EST. (13) The point of contact (POC) for this acquisition is Ms. Onyenauche Washington at (919) 541-0423/uche.washington@nih.gov.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIEHS/NIHES2016062/listing.html)
- Place of Performance
- Address: See SOW, United States
- Record
- SN03890124-W 20150918/150917000403-8c3bf6e4d85aa6c125390da9ee5c1017 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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