SOLICITATION NOTICE
Q -- Laboratory Testing to Measure PAH-related DNA Adduct Concentrations By 32P-postlabelling in DNA Extracted From Blood and Biopsy Samples
- Notice Date
- 8/18/2015
- Notice Type
- Presolicitation
- NAICS
- 621511
— Medical Laboratories
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E128, Rockville, Maryland, 20852, United States
- ZIP Code
- 20852
- Solicitation Number
- N02CP52669-76
- Archive Date
- 8/29/2015
- Point of Contact
- Catherine Muir, Phone: (240) 276-5434
- E-Mail Address
-
muirca@mail.nih.gov
(muirca@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Contracting Office Address: Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E144, Bethesda, MD 20892, UNITED STATES Description: National Cancer Institute (NCI), Div. of Cancer Epidemiology and Genetics (DCEG), Nutritional Epidemiology Branch (NEB), plans to procure on a sole source basis the laboratory services to measure PAH-related DNA adduct concentrations by 32P-postlabelling in DNA extracted from blood and biopsy samples from Rio Grande do Sul, Brazil from King's College London, 150 Stamford Street, London SE1 9NH, UK. The response close date of this notice for this requirement is in accordance with FAR 5.203(a)(1). This acquisition will be processed under FAR Part 12 - Acquisition for Commercial Items and in accordance with simplified acquisition procedures as stated in FAR Part 13.106-1(b)(1), and is exempt from the requirements of FAR Part 6. The North American Industry Classification System code is 621511 and the business size standard is $32.5M. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price, type contract. The period of performance will be twelve (12) months from date of award. It has been determined there are no opportunities to acquire green products or services for this procurement. The Division of Cancer Epidemiology and Genetics (DCEG) is a research program of the National Cancer Institute (NCI), one of the National Institutes of Health (NIH). The Division is the world's most comprehensive cancer epidemiology research group. Its renowned epidemiologists, geneticists, and biostatisticians conduct population and multidisciplinary research to discover the genetic and environmental determinants of cancer and new approaches to cancer prevention. The Division's research impacts public health policy in the United States and around the world. The mission of the Nutritional Epidemiology Branch (NEB) is to define the causal relationships between diet/nutritional status, energy balance, metabolism, lifestyle factors, and cancer. Our research encompasses diet, energy balance/obesity/physical activity, specific nutrients and supplements, diet-related additives, contaminants, metabolites, and intermediate biologic markers. Esophageal cancer is the sixth most common cause of cancer death in the world, and 80% of this cancer worldwide is esophageal squamous cell carcinoma (ESCC). The Nutritional Epidemiology Branch of the Division of Cancer Epidemiology and Genetics conducts research on the etiology of ESCC in high risk populations in several parts of the world including China, Iran, Kenya, and Southern Brazil. Drinking mate in Southern Brazil has been proposed as one of the plausible risk factors for the high risk of ESCC in these parts of the world, and polycyclic aromatic hydrocarbons (PAHs) are thought to be the responsible carcinogen in mate. Professor David Phillips' laboratory at King's College (London, UK) is set up to test PAH adducts in DNA samples (extracted from blood and biopsy) by 32P-postlabelling. The Contractor shall perform the following tasks: 1. Extract DNA from 245 esophageal biopsy samples. 2. Determine the PAH-related DNA adducts for two (2) samples (biopsy and blood) from 245 individuals, which totals 490 32P-postlabelling measurements. 32P-postlabelling analysis will be carried out using the nuclease P1 enrichment with some modifications. DNA (4 µg) already extracted from the samples will be digested using micrococcal endonuclease (0.25 U/ml) and spleen phosphodiesterase (2 mg/ml) for 3.5 hours at 37°C. DNA digests will be treated with nuclease P1 (0.96 µl, 1.25 mg/ml) for one hour at 37°C. To stop the nuclease P1-reaction, 1.92 ml Tris base (pH 9.6) will be added. Labelling of PAH-modified nucleotides will be done with [γ-32P] ATP plus polynucleotide kinase (PNK) (6 U) for 30 min at 37°C. The radiolabelled adducted nucleotide bisphosphates will be separated on PEI-cellulose sheets (Machery Nagel, Duren, Germany) by multidirectional thin layer chromatography (TLC). A standard with known levels of benzo[a]pyrene diol-epoxide(BPDE)-DNA adducts will be analyzed in parallel for quantification purposes. Quantification will be performed using an InstantImager (Canberra Packard, Dowers Grove, IL, USA). Assays will be done in duplicates with proper quality control samples, and the averages will be reported. 3. Communicate to the NCI Contracting Officer's Representative (COR) immediately via email or phone call if any unusual problems develop over the course of the project. 4. Deliver all data to the NCI COR as a report and tables as described herein. GOVERNMENT RESPONSIBILITIES: The Government shall coordinate the delivery of the 245 DNA samples extracted from blood, and 245 biopsy samples, to the Contractor for testing. The samples will be shipped to the Contractor directly from Brazil, and the NCI will only coordinate their shipment and delivery. Upon the receipt of the Contractors report, the NCI COR will examine the report data. REPORTING REQUIREMENTS AND DELIVERABLES: The Contractor shall provide a report of the methods used and the data generated from the postlabelling testing (hardcopy and electronic) to the NCI COR within thirty (30) days of the expiration of the Period of Performance INSPECTION AND ACCEPTANCE CRITERIA: The NCI will have fifteen (15) business days in which to review and accept the required deliverables. If no comments or request for revisions are provided within fifteen (15) business days the deliverables shall be considered acceptable. This laboratory service is provided by King's College London. The proposed Vendor has internationally renowned expertise in the measurement of aromatic DNA adducts using 32P-postlabelling. This laboratory is a pioneer in this assay, and has standardized the method through time, and is the only known laboratory that can perform all the studies required in the SOW. There are no other known sources that are capable of measuring aromatic PAH-related adducts with the same sensitivity, specificity, throughput, and demonstrated performance and quality as that of proposed Vendor. The NEB has used this laboratory with their specific methods for measurement of PAH-related DNA adduct levels on prior studies. The NEB's study is ongoing, and the performance characteristics and quality of the needed services, uniquely provided by the proposed Vendor, are required for maintaining the integrity of on-going studies. A change would be disruptive to the ability to analyze our cumulative results. This notice is not a request for competitive quotation. However, if any interested party, especially small businesses, believes it can meet the above requirement, it may submit a capability statement, proposal, or quotation, which shall be considered by the agency. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the NCI to determine if the party can perform the requirement. Responses must be received in the contracting office by 12:30PM EDT, on Aug. 28, 2015. All responses and questions must be in writing and faxed (240) 276-5401 or emailed to Catherine Muir, Contracting Officer via electronic mail at muirca@mail.nih.gov. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, Contractors must be registered and have valid, current Entity Record, including current Representations and Certifications, in the System for Award Management (SAM) through SAM.gov. Reference: N02CP52669-76 on all correspondence.
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