SOLICITATION NOTICE
66 -- HIDEX Sense Multi-Technology Microplate Reader
- Notice Date
- 8/18/2015
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334515
— Instrument Manufacturing for Measuring and Testing Electricity and Electrical Signals
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, Nat'l Institute of Diabetes, Digestive, & Kidney Diseases, 2 Democracy Plaza, Suite 700W, 6707 Democracy Blvd., MSC 5455, Bethesda, Maryland, 20892-5455
- ZIP Code
- 20892-5455
- Solicitation Number
- NIHLM2015618
- Archive Date
- 8/23/2015
- Point of Contact
- V. Lynn Griffin, Fax: 301-480-8501, MAXWELL KIMPSON,
- E-Mail Address
-
griffinv@mail.nih.gov, Max.Kimpson@nih.gov
(griffinv@mail.nih.gov, Max.Kimpson@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation number is NIHLM2015353 and is issued as a Request for Quotation (RFQ). The solicitation /contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2005-73. The North American Industrial Classification (NAICS) code 334519 with a size standard of 500 employees. This acquisition is being conducted using Simplified Acquisition Procedures in accordance with FAR Part 13. The National Institutes of Health (NIH), National Institute of Digestive, Diabetes & Kidney Diseases (NIDDK) has a requirement to procure Brand Name or Equal to: Lablogic Systems Inc., Hidex Sense Microplate Reader with the following specifications: HIDEX Sense Multi-Technology Microplate Reader for Luminescence (LUM), Fluorescence Intensity (Fl), Time Resolved Fluorescence (TRF), Fluorescence Polarization (FP) and Absorbance (ABS) measurements. Reader is robot accessible. Includes MS Windows based touch screen operated instrument control/data acquisition software and high performance optics for reading of micro-well plates up to 1536 wells. Features: Shaking prior to measurement of each plate or well Scanning of wells - 1 OOum steps over entire plate area Temperature Control from 2C above ambient to 65C Measurement time/plate - Depends on the plate and measurement type - Minimum <14 seconds for 96 well plate Light Source - Xe-flash lamp for Fl, TRF, FP and Abs modes - Excitation/wavelength range 230 nm - 1000 nm - Continuous closed loop intensity control Light Path - High intensity direct illumination optics - Filter storage for 32 15mm or 12. 7 mm fluorescence filters - Filter and dichroic mirror additions/changes through automatic hatch on instrument front Detectors Single PMT for detection for Fluorescence and Luminescence - Photon Counting for reading luminescence emission - Integral counting for Fl and FP - Photon counting with Time Resolved Decay Curve on TRF - Spectral range from 230 nm to 850 nm (PMT) Absorbance Mode - Spectrograph detector - Spectral range from 230nm to 1 OOOnm Fluorescence Mode - Filter storage for 32 Filters, 12.7 or 15 mm diameter - Wavelength Range: 230-850 nm - Kinetic and endpoint measurements. - Detection limits (> 3 S.D. above blank) - Dynamic range up to 6 decades. - Fluorescence Intensity (Fl): < 0.15 fmol fluorescein/ well in a black 384-well plate, 50uL sample vol. - Time-resolved Fluorescence (TRF): < 3 amol Eu/well in a white 384-well plate. 50uL sample vol. - Fluorescence Polarization (FP): < 5 (typical < 3 mP stdev) at 1 nM fluorescein, in a black 96 well plate, 200 uL sample vol. Luminescence Mode - Kinetic and endpoint measurements. - Glow Luminescence with or without filters - Dynamic range up to 6 decades - Sensitivity < 10 pM glow luminescence in a white 96-well plate (> 3 S.D. above blank) - Background < 200 CPS - Cross-talk (sample volume 200 uL): <0.01 % on white 96-well plate, sample vol. 200 ml. <0.5% on white 384-well plate, sample vol. 50ml. Absorbance Mode Kinetic and endpoint measurements Wavelength Range 230-1000 nm Performance at 450nm using 200uL sample volume: Accuracy 1% at 20D Repeatability 0.8% at 20D Dynamic Range 4.0 OD on 96 well plate Optical Filters included: Filter 330nm for TRF-Eu & TR-FRET Filter 620nm for TR-FRET Eu Filter 616nm for TRF-Eu & TR-FRET Filter 665nm for TR-FRET Filter 355nm for Umbelliferone Filter 485nm for Fluoro FITC Filter 544nm for Rhodamine Filter 460nm for Umbelliferone & BRET Filter 535nm for Fluoro FITC & BRET The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), has a requirement to carry out studies of glucose metabolism on a cellular and systemic level in cell culture, tissues, and whole animal that require the use of fluorescent, luminescent, and absorbance assays in multi-well plates. As part of our overall studies on the interplay between Epo and glucose metabolism, the ability to quickly and accurately measure a variety of compounds is crucial in better understanding glucose metabolism. The system must be quantitative and automated to provide for consistent and reproducible results. A small footprint and light weight for portability are also required due to space limitations and the need for mutli-researcher use. The microplate reader system should be automated with a high performance multimode microplate reader, compatible with 1-1536 well plates featuring five detection modes with top and bottom read. The system should also include temperature control and microplate shaking. The system needs an optical filter system with the capacity for 32 optical filters for a wide variety of fluorescent and luminescent assays without manual filter replacement. Absorbance assays need to scanned over a large wavelength spectrum in a minimal amount of time. The microplate reader needs to have an integrated computer interface that offers ease-of-use and comes with the option of mouse/keyboard and touchscreen capabilities. The system should have state-of-the-art technology to produce accurate and reliable results in a quick and efficient manner. All hardware and software components need to be of recent design using solid-state electronics and modern robotics. The Hidex Sense Microplate Reader is a compact, application ready high performance multimode microplate reader, compatible with 1 to 1536 well plates, featuring five detection modes with top and bottom read and automatic height adjustment. The system also includes temperature control (ambient + 4 °C to 65°C), which allows for a wider range of temperature experiments, and versatile microplate shaking that ensures consistency between plates, runs, and experiments. This system updates our current system and provided greater flexibility to accommodate our many assays for cell analysis such as viability, calcium uptake, enzymatic activity, and enzyme-based quantification of specific proteins. An optical filter system with large on-board capacity (32 filters) enables direct readiness with optimal performance for a wide variety of fluorescent and luminescent assays, without manual interaction. Absorbance assays are measured utilizing a spectrograph detector, providing complete (230nm0100nm)spectrum readout and in less than a second per sample. Several wavelengths can be measured simultaneously. This greatly expands the number of assays that can be carried out without manually changing the filters and increases the information output by providing a complete spectral scan, important in hemoglobin analysis. The offeror must include a completed copy of the following provisions: 1) FAR Clause 52.212-1 Instructions to Offerors - Commercial items; 2) FAR Clause 52.212-2, Evaluation - Commercial Items. As stated in FAR Clause 52.212-2 (a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be advantageous to the Government, price and other factors considered. The following factors will be used equally to evaluate offers: Technical Evaluation, Price, and Past Performance. Note: Past Performance Information: Vendors must submit a listing of the most recent contracts/awards (minimum of 3) which demonstrate similar work in nature to this Solicitation. Contracts/awards may include those entered with the Federal Government, state and local governments and commercial concerns. Include the following information for each contract or subcontract: 1.Name of Contracting Organization 2.Contract Number (for subcontracts provide the prime contract number and the subcontract number) 3.Contract Type 4.Total Contract Value 5.Description of Requirement 6.Contracting Officer's Name and Telephone Number 7.Program Manager's Name and Telephone Number 3) FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items; 4) FAR Clause 52.212-4, Contract Terms and Conditions - Commercial Items; 5) FAR Clause 52-212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items - Deviation for Simplified Acquisitions. The Dun and Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN) and the certification of business size shall be included. The clauses are available in full text at https://www.acquisition.gov. PLEASE NOTE: In order to receive an award, contractor must be registered and have valid certification in the System For Award Management (SAM) http://www.sam.gov Interested vendors capable of providing the Government with the items specified in this synopsis should submit their quotation to the below address. Quotations will be due on or before August 22, 2015 at 11:00 a.m. EST. Offersors shall provide an original and one copy of your quotation. The quotation must reference Solicitation number NIHLM2015618. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. Quotations must be submitted electronically to griffinv@mail.nih.gov
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDDKD/NIHLM2015618/listing.html)
- Record
- SN03843366-W 20150820/150818235657-3a21026c6ece5bcbd73f6df944b978b1 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |