SOLICITATION NOTICE
B -- Functional impact of UDP patients NCAPD2 variants on chromosome aberrations
- Notice Date
- 8/6/2015
- Notice Type
- Presolicitation
- NAICS
- 611310
— Colleges, Universities, and Professional Schools
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- HHS-NIH-NHLBI-CSB-(HG)-2015-203-DLM
- Archive Date
- 8/28/2015
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- INTRODUCTION: THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) intends to negotiate and award a purchase order on a noncompetitive sole source basis to University of Michigan, Molecular, Cellular, and Developmental Biology, 830 N. University Avenue, Ann Arbor, Michigan 48109-1048 to examine chromosome aberrations arising during cell division in C. elegans, benefiting from the experimental ease of engineering patient variant and the facile interrogation of chromosome aberrations at both the cytological and genomic levels for the National Institute of Health (NIH), National Human Genome Research Institute (NHGRI). Background: The sole source determination is based on the fact that the National Institute of Health (NIH) mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability. The National Human Genome Research Institute (NHGRI), mission has expanded to encompass a broad range of studies aimed at understanding the structure and function of the human genome and its role in health and disease. The National Human Genome Research Institute (NHGRI), Undiagnosed Disease Program (UDP) is a program designed to address the needs of persons with debilitating medical conditions for which no diagnosis has been found despite an extensive workup. The goals of the UDP include finding accurate diagnoses and discovering new diseases that provide insight into human physiology and genetics. The UDP was established to evaluate participants who have undiagnosed medical conditions. UDP participants have generally received extensive diagnostic workups before traveling to the NIH and often have numerous and/or complex medical problems. The UDP program makes use of a diverse set of diagnostic and research techniques both to attempt to find a diagnosis and to generate research projects to advance medical knowledge. In order to determine the function of 2 novel UDP patient mutations in NCAPD2 and how they lead to the pathogenicity of disease, the laboratory of Dr. Gyorgyi Czankovszki at the University of Michigan, Department of Molecular, Cellular and Developmental Biology will generate C. elegan models that contain the homologous F1278T and S1370Y mutations via CRISPR-Cas technology and analyze these strains for chromosomal alterations. This work will continue to support the UDP by providing functional analysis of suspected gene mutations to determine how they generate the unique disease phenotype seen in UDP patients. Purpose and Objectives: The purpose of this acquisition is to provide functional analysis for 2 mutations in the NCAPD2 gene seen in UDP patients by examination of patient specific transgenic C. elegans to analyze cytological and sub-microscopic chromosome aberrations. Specifically, this laboratory supports the research needs of the UDP by collaborating on particular clinically identified new and rare patient disease Period of Performance: 12 Months Upon Award Justification: Dr. Csankovski and the laboratory at the University of Michigan, Department of Molecular, Cellular and Developmental Biology has previous expertise in performing functional analysis of mutations and studying the relevance of these mutations in chromosomal segregation and structure by using the model organism of C. elegans. The knowledge gained from her previous studies, will assist the UDP in providing patient specific functional screening to determine chromosomal changes caused by the NCAPD2 variants. The contractor shall provide the following: • The contractor shall generate C. elegan models that contain the homologous mutations of the UDP patient. • The contractor shall introduce strains containing individual and compound heterozygous patient specific mutations into appropriate GFP-histone and/or GFP plasma membrane background strains. • The contractor shall perform time-lapse microscopy to examine embryonic cell divisions. • The contractor shall examine chromosomal defects by immunofluorescence microscopy on fixed tissues. • The contractor shall perform whole genome and comparative sequencing to analyze sub-chromosomal aberrations in the presence of each patient mutation in a homozygous state. • The contractor shall supply electronic copies of all reports appropriate for deposition in the UDP process management system and for other collaborations. Regulatory Authority: This acquisition is conducted under the authority of the Federal Acquisition Regulations (FAR) Subpart 13.106-1(b) Soliciting from a single source. Additional Information: Industry Classification (NAICS) Code is 611310, Colleges, Universities and Professional Schools with size standard in dollars $27.5M Per Annum. The acquisition is being conducted under FAR Part 13, Simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001). The resultant Contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-83 (July 2, 2015). This requirement is under the SAT of $150,000.00. This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must be received no later than 7:30 a.m. Eastern Standard Time (EST), August 13, 2015; and must reference synopsis number HHS-NIH-NHLBI-CSB-(HG)-2015-203-DLM, may be submitted to the National, Heart, Lung and Blood Institute, COAC Services Branch, Office of Acquisitions, Office of Management, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative. "All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency."
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