SOLICITATION NOTICE
66 -- Brand Name or Equal Zeiss LSM780 NLO Laser Scanning Confocal Microscope
- Notice Date
- 8/25/2014
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Bldg 1050, Frederick, Maryland, 21702, United States
- ZIP Code
- 21702
- Solicitation Number
- N02RC42572-57
- Point of Contact
- Reyes Rodriguez, Phone: 240-276-5442, Seena Ninan, Phone: 240-276-5419
- E-Mail Address
-
reyes.rodriguez@nih.gov, ninans@mail.nih.gov
(reyes.rodriguez@nih.gov, ninans@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- I. Description of Requirement The NCI, CCR, Experimental Immunology Branch (EIB) provides a number of microscopes to support the imaging needs of researchers. The new laser scanning confocal microscope is needed to provide state of the art imaging technologies for EIB researchers, and to replace a 13 year old, and now obsolete, confocal microscope. The following features are necessary and unique to this particular instrument: • Inverted Microscope stand with Fully motorized 6 position compatible objective nosepiece and 6 position push and click motorized fluorescence filter turret • Motorized integrated Z focus with a step size of 10nm and travel range of 25mm • TFT touch panel independent of microscope stand with focus control and access to all microscope motorized functions. Can be placed conveniently by computer and monitors. • High Efficiency Hard Coated Shift Free filters for GFP, CY3, DAPI for Standard Visual Fluorescence Imaging. • High Resolution DC Closed Loop Scanning Stage with Joystick and Integrated Computer Control including Universal Specimen Holder Suitable for variety of slide and round dishes. • Definite Focus - Integrated focus which maintains focus during long term live cell imaging. Features of High Sensitivity Laser Scanning Confocal Microscope: • Must contain a 34-Channel Quasar(Spectral) GaAsP Detector, consisting of calibrated 32-Array GaAsp and two flanking single PMT detectors, enabling image acquisition with freely definable emission spectra and advanced spectral acquisition and analysis Spectral GaAsp detector allows increased QE(>45%) over standard PMT detectors, critical for our low light fluorescence applications. • System must include the following laser lines 355, 405, 458,488,514,561,594, and 633 • Hardware must support simultaneous acquisition from 34 channels plus TPMT and 2 additional detectors for up to 37 simultaneous 16 bit inputs. • Spectral GaAsP should allow image collecting in photon counting mode for single molecule applications or integration mode for standard fluorescence imaging. • Incorporates Primary Dichroioc Beam Splitter(Twin Gate) at 10 degree position, you achieve Extremely HIGH laser suppression on the order of 6-7 OD allowing superb reflection free imaging. The System omits all secondary dichroics for high efficiency emission collection. • Point Scanner must obtain at least 8 fps at 512x512 and e.g. 250 fps at 512x16 • Digital gain (noise free contrast enhancement prior to bit depth conversion).in addition to standard master gain pedestal. • Free selection of emission bands for detection, omitting secondary dichroics or emission filters. Must be possible to set the emission bands over laser lines and still have efficient laser blocking to obtain superior signal to noise with low light fluorescent samples. • Freely rotatable scan field (0-360 degree in 0.1° steps) with user definable size and shape due to independent galvo-mirrors. • Multiple (up to 99) freely definable real regions of interest (rROIs). Pixel precise control of the laser intensity and signal detection. Prevention of photo damage outside the rROIs boundaries necessary for work on living cells, FRAP, uncaging, photoactivation and photoconversion experiments. • Individual AOTF settings (line selection and attenuation) per rROI. • System must offer at least 14 different scan speed levels (28 levels including bi-directional scans) for the wide variety of specimens and applications we will design. This is especially critical for our single molecule experiments. • It must be possible to select different scan speeds for bleach ROIs and image frame. • Variable scan field size (hardware-zoom) from 0.6 to 40 in 0.1 steps for magnifications until the optical limits of resolution, with a scan field of 20 mm diagonally in the intermediate image plane. • Image format from 4x1 to 6144x6144 pixels in up to 35 detection channels simultaneously with up to 16 bit depth (65536 gray values) in all channels. (8, 12 OR 16 bit user selectable bit depth.) • Multitracking for the prevention of cross-talk by linewise or framewise switching of the laser excitation line, essential for multi-fluorescence applications and co-localization studies. • Advanced time-series module with automatic bleach and direct trigger-control to design the entire experimental protocol. All scan strategies can be applied (line, spline, frame, z-line, z-spline, z-stack, rROIs, z-rROIs) in the time series. • Absolute linear scanner movement to ensure equal pixel dwell-times as a prerequisite for any quantitative studies. • Calibration and linearization of the 32-Array GaAsp detector and the two flanking single PMTs, all 34-channels can be used for spectral acquisition and analysis. • 34-Channel GaAsP detector (GaAsp-array with 32 PMTs and two flanking single PMTs) in combination with the highly efficient holographic grating to provide an even spectral dispersion over the entire visual spectral range. • Spectral recycling loop efficiently recovers non-separated light, redirecting it onto the holographic grating. • Fast parallel acquisition in one single scan of the entire spectral range as a lambda stack (391-749 nm) covered by the 34-Channel QUASAR Detector. (Suitable for spectral analysis in living cells, e.g. colocalization studies and FRET-experiments). Up to 10 different fluorescent dyes can be imaged and separated simultaneously. • Spectral bandwidth for lambda stack acquisition to be selectable by the user. A full spectral image is used for unmixing (suitable for spectral imaging of living cells and later unmixing without any spectral distortions). • Reproducible high resolution spectral data acquisition with 3.2 nm or 4.9 nm steps through sequential spectral scanning in addition to the 10nm parallel spectral scanning. • Online Fingerprinting: Image channel definition not by emission bands but by reference spectra and live processing unmixing during image acquisition. • Spectra that can be created from a Lambda stack. • Free combination of manually defined, automatically extracted and spectra stored in the database is possible to perform unmixing on a lambda stack. • Potential to use the entire emission spectrum for dyes for detecting with much higher signal to noise ratios (in contrast to detection with bandpass techniques). • Optics designed for high transmission and aberration free imaging across the near UV to visible range, 350 - 1100 nm. • Light sensitive external BiG GAsP NDD detector for multi-photon imaging • Filter set BiG+ for green-red NLO imaging • Beam routing optics for NLO imaging using Coherent Chamaleon Vision II laser • Acquisition software includes multi-time/dimensional acquisition with multi-parametric experiment designer and both tile and position array settings. • Must include at least a 12 months warranty on parts and labor • Parts must be compatible with currently owned Zeiss AxioObserver Z1 wide-field microscope. II. Delivery Contractor(s) shall deliver the item within sixteen (16) weeks after award to NIH/NCI Bethesda, Maryland 20892. The Contractor that receives the award will be provided with the complete mailing address and point of contact. Upon delivery, contractor must notify the NCI Contracting Officer's Representative (COR) to schedule the installation date and time. III. Installation and Training To be carried out by the manufacturer and shall occur within approximately one week of delivery. IV. Payment Payment shall be made after delivery, installation, and successful operation of the microscope. Payment authorization requires submission and approval of invoice to the NCI COR and NIH Commercial Accounts. QUOTATIONS ARE DUE: September 2, 2014 at 11:00 am, EST. PROVISIONS AND CLAUSES: The following provisions and clauses will be incorporated by reference: 52.211-6 Brand Name or Equal (a) If an item in this solicitation is identified as quote mark brand name or equal, quote mark the purchase description reflects the characteristics and level of quality that will satisfy the Government's needs. The salient physical, functional, or performance characteristics that quote mark equal quote mark products must meet are specified in the solicitation. (b) To be considered for award, offers of quote mark equal quote mark products; including quote mark equal quote mark products of the brand name manufacturer, must- (1) Meet the salient physical, functional, or performance characteristic specified in this solicitation; (2) Clearly identify the item by- (i) Brand name, if any; and (ii) Make or model number; (3) Include descriptive literature such as illustrations, drawings, or a clear reference to previously furnished descriptive data or information available to the Contracting Officer; and (4) Clearly describe any modification the offeror plans to make in a product to make it conform to the solicitation requirements. Mark any descriptive material to clearly show the modification. (c) The Contracting Officer will evaluate quote mark equal quote mark products on the basis of information furnished by the offeror or identified in the offer and reasonably available to the Contracting Officer. The Contracting Officer is not responsible for locating or obtaining any information not identified in the offer. (d) Unless the offeror clearly indicates in its offer that the product being offered is an quote mark equal quote mark product, the offeror shall provide the brand name product referenced in the solicitation. (End of provision) 52.212-1 Instruction to Offerors Commercial Items 52.212-2, Evaluation Commercial Items: The purchase order will be awarded to the Lowest Price Technically Acceptable (LPTA). The award will be made on the basis of the lowest evaluated price of quotations meeting or exceeding the acceptability standards for the requirements/technical specifications stated above. The technical evaluation will be a determination based on information furnished by the vendor. The Government is not responsible for locating or securing any information which is not identified in the proposal. The Government reserves the right to make an award without discussions. 52.212-3 Offerors Representations and Certifications Commercial Items (November 2013) WITH DUNS NUMBER ADDENDUM (52.204-6 (July 2013): 52.212-4 Contract Terms and Conditions Commercial Items, applies to this acquisition. 52.247-34 FOB Destination 52.212-5 Contract terms and Conditions Required to Implement Statutes or Executive Orders---Commercial Items (January 2014). The following additional FAR clauses cited in this clause are applicable: (b) The Contractor shall comply with the FAR clauses in this paragraph (b) that the Contracting Officer has indicated as being incorporated in this contract by reference to implement provisions of law or Executive orders applicable to acquisitions of commercial items: 52.203-6, Restrictions on Subcontractor Sales to the Government, with Alternate I (41 U.S.C. 253g and 10 U.S.C. 2402) 52.203-13, Contractor Code of Business Ethics and Conduct 52.204-10 Reporting Executive Compensation and First Tier Subcontract Awards 52.209-6, Protecting the Government' Interest When Subcontracting with Contractors Debarred, Suspended, or Proposed for Debarment 52.209-9, Updates of Publicly Available Information Regarding Responsibility Matters 52.219-4 Notice of Price Evaluation Preference for HUBZone Small Business Concerns 52.219-8 Utilization of Small Business Concerns 52.219-28 Post Award Small Business Program Representation 52.222-3, Convict Labor 52.222-19 Child Labor Cooperation with Authorities and Remedies 52.222-21 Prohibition of Segregated Facilities 52.222-26 Equal Opportunity 52.222-35 Equal Opportunity for Veterans 52.222-36 Affirmative Action for Workers with Disabilities 52.222-40 Notification of Employee Rights Under the National Labor Relations Act 52.222-37 Employment Reports on Veterans 52.223-18, Encouraging Contractor Policies to Ban Text Messaging while Driving 52.225-1 Buy American Act - Supplies 52.225-3, Buy American Act--Free Trade Agreements--Israeli Trade Act 52.225-5 Trade Agreements 52.225-13 Restrictions on Certain Foreign Purchase 52.232-33 Payment by Electronic Funds Transfer System for awards Management 52.247-64, Preference for Privately-Owned U.S. Flag Commercial Vessels 52.246-2 Inspection of Supplies-Fixed Price Full text copies of the representations and certifications for other cited provisions and clauses many be obtained on line at the NCI website at http://rcb.cancer.gov/rcb-internnet/index.jsp or from Reyes Rodriguez, Contract Specialist at reyes.rodriguez@nih.gov. Quotations must be received in the NCI-OA contracting office by 11:00 a.m. EST on September 2, 2014. Please refer to the solicitation number N02RC42572-57 on all correspondence. Faxed or electronic mail quotations will be accepted. OFFERORS: Offers must be submitted on an SF-1449 with a completed 52.212-3 Offeror Representations and Certifications-Commercial Items-with DUNS Number Addendum, signed by an authorized representative of the offeror OR provide a copy of the valid certification registrations of the offeror's Central Contractor Registration (CCR) and Online Representations and Certifications Applications (ORCA) through sam.gov. In order to receive an award, contractor must be registered and have valid certification in the Central Contractor Registration (CCR) and the Online Representation and Certifications Applications (ORCA) through sam.gov.
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FBO.gov Permalink
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- Place of Performance
- Address: TBD, United States
- Record
- SN03479521-W 20140827/140826000717-5c41c2d31682448a92d8585d601033f3 (fbodaily.com)
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