SOLICITATION NOTICE
B -- Library Preparation and Sequencing of 167 total RNA samples
- Notice Date
- 7/31/2014
- Notice Type
- Presolicitation
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NIH-HHS-NHLBI-CSB-(HG)-2014-206-DLM
- Archive Date
- 8/23/2014
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- INTRODUCTION : The National Institute of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) on behalf of the National Human Genome Research Institute, (NHGRI), intends to negotiate and award a purchase order on a noncompetitive sole source basis to Axeq Technologies, 9700 Great Seneca Highway, Rockville, Maryland 20850 to procure the following: Procurement: 1. Library preparation and sequencing of 167 total RNA samples; 2. Library preparation will be done using the TruSeq stranded total RNA with Ribo-Zero kits (cytoplasmic ribosomal RNA removal) 3. Sequencing will be performed using the latest Illumina HiSeq 2000 systems and other related instrumentation and software with 100-bp paired-end sequencing method; and 4. The raw data and analyzed data will be provided as the deliverables. The analyzed data will only include: Mapping to human reference genome. BACKGROUND: The National Institutes of Health (NIH) is the nation's leading medical research agency and the primary Federal agency conducting and supporting medical discoveries that improve people's health and save lives. The National Human Genome Research Institute (NHGRI), National Institutes of Health Undiagnosed Disease Program (UDP) is a trans-National Institute of Health (NIH) initiative that focuses on the most puzzling medical cases referred to the NIH Clinical Center in Bethesda, MD. It was organized by the National Human Genome Research Institute (NHGRI), the NIH Office of Rare Diseases Research (ORDR) and the NIH Clinical Center. Many medical specialties from other NIH research centers and institutes contribute the expertise needed to conduct the program, including endocrinology, immunology, oncology, dermatology, dentistry, cardiology and genetics, among the dozens of participating senior attending physicians. A longstanding medical condition that eludes diagnosis by a referring physician can be considered undiagnosed and may be of interest to this clinical research program. identified new and rare diseases. Objectives: The UDP program makes use of a diverse set of diagnostic and research techniques both to attempt to find a diagnosis and to generate research projects to advance medical knowledge. Axeq has research knowledge in molecular biology, cell biology, genetics research, and the use of cutting edge research tools including geneotyping, Sanger sequencing and next-generation sequencing platforms that are used by UDP for their clinical and protocol study research. Axeq already possess the knowledge and experience with UDP patient samples and previously have provided transcriptome sequencing. Period of Performance : 12 Months Upon Award JUSTIFICATION: The NIH Undiagnosed Diseases Program requires Axeq to perform transcriptome sequencing using the Illumina HiSeq 2000 system with 100 million reads per sample in order to perform SNP and Indel analysis on patient and familial RNA to assist in determining the causative mutation in the patient. The sequencing instrumentation parameters, chemistry, and report data format must remain consistent with transcriptome results previously obtained in order to perform analyses with data using programs developed in-house and to allow for direct comparison for ongoing research. Additionally, Axeq Technologies has the capability to perform transcriptome sequencing with the following UDP requirements : • Library preparation and sequencing of 167 total RNA human samples from the NIH Undiagnosed Diseases Program; • 100 million individual reads per samples with the equivalence of 50 million paired-end reads and 10 Gb of raw data; • Library preparation must be performed using TruSeq stranded total RNA with Ribo-Zero kits; • Transcriptome sequencing must be performed using the Illumina HiSeq 2000 systems and other instrumentation and software leading to 100- bp paired end sequencing method; • Analyzed data will include the following: o Mapping to the human reference genome o Individual gene expression counts o SNP and Indel calls o Annotated SNPs • Turnaround time must be 8 weeks are less after samples have passed Macrogen's initial QC. • Data must be available through secure FTP download or shipped to the UDP on a hard disk. REGULATORY AUTHORITY: This acquisition is conducted under the authority of the Federal Acquisition Regulations (FAR) Subpart 13.106-1(b) Soliciting from a single source, only one responsible source and no other supplies or services will satisfy agency requirements. ADDITIONAL INFORMATION : Industry Classification (NAICS) Code is 541711, Research and Development in Biotechnology, with a business size standard of 500. The acquisition is being conducted under FAR Part 13, simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001). The resultant Contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-74 (July 1, 2014). This requirement is under the SAT of $150,000.00. This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must be received by August 8, 2014 and must reference synopsis number HHS-NIH- NHLBI-CSB-(HG)-2014-206-DLM, may be submitted to the National, Heart, Lung and Blood Institute, Office of Acquisition, COAC Services Branch, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative. "All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency."
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NIH-HHS-NHLBI-CSB-(HG)-2014-206-DLM/listing.html)
- Place of Performance
- Address: Contractors Location and NIH, United States
- Record
- SN03444671-W 20140802/140731235315-e32266f080f1f8432bbbbdda67480fa4 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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