MODIFICATION
A -- RNA Sequencing
- Notice Date
- 6/27/2014
- Notice Type
- Modification/Amendment
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Other Defense Agencies, Uniformed Services University of the Health Sciences, UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES (USUHS), 4301 JONES BRIDGE ROAD, Bethesda, Maryland, 20814-4799, United States
- ZIP Code
- 20814-4799
- Solicitation Number
- HU0001-14-Q-0114
- Archive Date
- 7/2/2014
- Point of Contact
- Michelle Creenan,
- E-Mail Address
-
michelle.creenan@usuhs.edu
(michelle.creenan@usuhs.edu)
- Small Business Set-Aside
- N/A
- Description
- The purpose of this amendment is to answer questions received and extend the due date for quotes to 10:00 PM eastern local time, July 1st. All other terms and conditions remain the same. Q: What sequencing depth does the client require for this program? For example, identifying rare transcripts will require significantly deeper sequencing, which will affect cost per sample. Alternatively, a pricing matrix could be provided to allow the client to make a price/depth decision after receiving a proposal. Would this be preferred? A: We expect that the process would provide significant depth of sequencing that would identify rare sequences from a fairly homogeneous sample of between 500 nanograms to 1 microgram of total RNA sample submitted. Q: Is the client interested in small RNAs (i.e. miRNAs) as well as a separate sequencing service, or only mRNA transcripts? A: We will provide total RNA from which we would like mRNA sequencing only. Micro-RNA (miRNA) sequencing is not required. Q: What is the "sample type" that will be delivered to the performing lab? Purified RNA? Cell lysates? Cell pellets? A: We will provide the vendor roughly 1 microgram of purified total RNA from both controls and treated samples.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/notices/77fb1fd6d6c382f3d664122e9d6d8b40)
- Place of Performance
- Address: 4301 Jones Bridge Road, Bethesda, Maryland, 20814, United States
- Zip Code: 20814
- Zip Code: 20814
- Record
- SN03409305-W 20140629/140627235214-77fb1fd6d6c382f3d664122e9d6d8b40 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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