SOLICITATION NOTICE
66 -- MiSeq NextGen Sequencing System
- Notice Date
- 6/25/2014
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Agricultural Research Service - Midwest Area
- ZIP Code
- 00000
- Solicitation Number
- AG-5615-S-14-BB02
- Archive Date
- 7/20/2014
- Point of Contact
- Dolores J Milton, Phone: 6088900045
- E-Mail Address
-
dolores.milton@ars.usda.gov
(dolores.milton@ars.usda.gov)
- Small Business Set-Aside
- N/A
- Description
- This notice is a combined synopsis/solicitation, Notice of Intent. The Midwest Area for the Agricultural Research Service of the United States Department of Agriculture intends to issue an award to Illumina, Inc., 5200 Illumina Way, San Diego, CA 92122. This is a sole source procurement under the authority of FAR 6.302-1. Only one responsible source and no other supplies or services will satisfy agency requirements. This is a requirement of the Cereal Crops Research Unit (CCRU), USDA, ARS, MWA, Madison, WI for the vendor to provide a MiSeq NGS (NextGen Sequencing) System Cat# SY-410-1003) for DNA and RNA sequencing. Minimum requirements include: 1) Nucleic Acid Desktop Sequencer capable of taking direct introduction of quantitated DNA. 2) Performing amplification, sequencing, and data analysis in a single component system. 3) Fully automated generation of sequence using bridge amplification-to-sequencing automated workflow rather than physical shearing. 4) Emulsion PCR and bead quantitation that requires additional, ancillary equipment adding both to the cost and time spent processing. 5) Machine must be capable of producing both single and paired end reads with a high quality score: 2 x 150 bp, greater than 80% of the bases generated must be of a Q30 quality score or better. 6) Machine must have a workflow that can be easily adjusted to match our varying throughput needs. 7) Machine must be tunable, allowing a choice of runtimes, output (from 450Gb to 15Gb). 8) Kit configuration allowing the user to determine the configuration of the instrument for various (DNA, RNA, methylation, GBS, targeted resequencing, 16s metagenomics, etc) applications or to favor speed, accuracy, or cost, depending on priority. 9) Cereal Crops have very large and complex genomes and therefore the machine must provide robust high quality sequence reads that are minimally impacted by complex elements such as homopolymer runs (Loman et al., Nature Biotech, 2012) and repetitive elements. Interested parties should submit written (mail or email) responses including prices and technical data sufficient to determine capability to meet the entire requirement and must be received in this office within 10 calendar days after publication of this notice. It should be noted that no contract will be awarded on the basis of offers received in response to this notice, but will be used to determine if a comparable source more advantageous to the Government is available. If no affirmative written responses are received, negotiations will be held with Illumina, Inc. without further notice. This acquisition is being conducted under the Federal Acquisition Streamlining Act, Simplified Acquisition Procedures. SOLE SOURCE JUSTIFICATION: Upon the basis of the following justification, I, as Contracting Officer, hereby approve the use of other than full and open competition for the proposed contractual action pursuant to the authority of 10 U.S.C.2304(c)(1), FAR Subpart 6.302-1(a)(2)(iii), Only one responsible source and no other supplies or services will satisfy agency requirements. JUSTIFICATION 1. Agency and Contracting Activity (FAR 6.303-2(a)(1)). USDA, AGRICULTURAL RESEARCH SERVICE, Midwest Area, Procurement & Property Section 2. Nature/Description of Action(s) (FAR 6.303-2(a)(2)). a. This contracting action is to obtain a MiSeq NextGen Sequencing System to perform DNA and RNA sequencing of Cereal Crops. 3. Description of Supplies/Services (FAR 6.303-2(a)(3)). MiSeq Sequencing will be used to produce high quality reads of both targeted and whole genome targets in cereal grains. Furthermore RNA-Seq will be used to evaluate the RNA abundance in Barley cell types as well as rare transcript discovery. The MiSeq machine must be delivered to the customer within 30 days after placing the order. 4. Identification of Statutory Authority (FAR 6.303-2(a)(4)). 10 U.S.C. 2304 (c)(1), FAR Subpart 6.302-1. The services required by the agency are available from only one responsible source and no other type of services will satisfy the Agency requirements. 5. Demonstration of Contractor's Unique Qualifications (FAR 6.303-2(a)(5)). a. Only one responsible Source and No Other Supplies or Services will satisfy Agency Requirements (FAR 6.302-1(a)(2)) (1) FAR 6.302-1(a)(2) states, "When the supplies or services required by the agency are available from only one responsible source, or for DOD, NASA, and the Coast Guard, from only one or a limited number of responsible sources, and no other type of supplies or services will satisfy agency requirements, full and open competition need not be provided for." (2) The named vendor is the only producer of the MiSeq NextGen Sequencing system and the MiSeq is the sole Desktop Sequencer that satisfies all our requirements, according to our specifications. 6. Federal Business Opportunities - FedBizOpps.gov (FBO) Announcement/Potential Sources (FAR 6.303-2(a)(6). a. A Federal Business Opportunities (FedBizOpps) synopsis was published in 2008 in an attempt to identify other possible vendors of the required SNP's. b. Illumina was the only qualified source that responded. 7. Determination of Fair and Reasonable Cost (FAR 6.303-2(a)(7). After a thorough web search, no other suppliers were located that distribute the MiSeq System. 8. Description of Market Research (FAR6.303-2 (a)(8). USDA/ARS is constantly surveying the market and from this experience knows that the service is proprietary and only available from the vendor identified. 9. Any Other Supporting Facts (FAR 6.303-2(a)(9)) Due to the proprietary nature of the item needed to perform the research, at this time there is only one source available. 10. Listing of Interested Sources (FAR 6.303-2(a)(10). No other sources are available at this time. 11. Actions Taken to Remove Barriers to Competition (FAR 6.303(a)(11)). Vendor surveys are done from time to time to see if other existing items can meet the research requirements 12. Technical Requirements and Certifications; (FAR 6.303-1(b) and FAR 6.303-2(b) I certify that the facts and representations under my cognizance which are included in this justification and which form a basis for this justification are complete and accurate. CONTRACTING OFFICER CERTIFICATION: (FAR 6.303-1(A), 6.303-2(A)(12) I certify that this justification is accurate and complete to the best of my knowledge and belief. //s// Name: Dolores Milton Title: Contracting Officer
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/notices/644f0d895152a539acf2cf31737d877c)
- Place of Performance
- Address: USDA-ARS, US Dairy Forage Research Center, 1925 Linden Drive, Madison, Wisconsin, 53706-1108, United States
- Zip Code: 53706-1108
- Zip Code: 53706-1108
- Record
- SN03406245-W 20140627/140625235321-644f0d895152a539acf2cf31737d877c (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |