SOLICITATION NOTICE
B -- Development of a mouse model for Merkel Cell Carcinoma
- Notice Date
- 8/27/2013
- Notice Type
- Presolicitation
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Bldg 1050, Frederick, Maryland, 21702, United States
- ZIP Code
- 21702
- Solicitation Number
- NCI-130119-TK
- Point of Contact
- Tiffany D. Kittrell, Phone: 2402765434
- E-Mail Address
-
Tiffany.kittrell@nih.gov
(Tiffany.kittrell@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Title: Development of a mouse model for Merkel Cell Carcinoma Document Type: Presolicitation Notice Solicitation Number: NCI-130119-TK Proposed Posted Date: August 27, 2013 Proposed Response Date: September 10, 2013 Classification Code: B-Special studies and analysis NAICS Code: 541380- Testing laboratories Business Size Standard: $14 Million Description The National Cancer Institute (NCI) Dermatology Branch plans to procure on a sole source basis the services to develop a mouse model of Merkel Cell Carcinoma from Applied Stem Cell at 1165 O'Brien Drive, Suite A Menlo Park, CA 94025. The products herein are being procured in accordance with the simplified acquisition procedures as authorized by FAR Part 13.106-1 (b) (1). The North American Industry Classification System code is 541380 and the business size standard is 14 million. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. Period of Performance: One year from the time of award. The NIH, NCI, Dermatology Branch, Cutaneous Development and Carcinogenesis Section, work on Merkel Cell Carcinoma as well as studying normal Merkel Cell development and Merkel Cell Stem cells in skin. Mouse models are powerful means of studying skin tissue, and the laboratory has many mouse models to study normal Merkel Cell development in skin. The two mice NCI would like to have generated will be very useful in studying Merkel Cell Carcinoma. Specifically, by crossing these mice NCI will be able to study the effect of Merkel Cell Polyomavirus large T antigen under the control of the Cytokeratin 18 promoter in an inducible system. This system will be important for further understanding of the mechanisms involved in Merkel Cell Polyomavirus T antigen driven cancers and will provide a model for further study of pathways involved in the development of this aggressive disease. The purpose of this acquisition is to develop a mouse model of Merkel Cell Carcinoma which is critical for further study of the pathways involved in the development of this disease. This project requires the use of TARGATT technology. TARGATT, which is proprietary technology to Applied Stem cell, was developed specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene can be inserted efficiently into a defined, transcriptionally-active locus with high gene expression in a single copy fashion. Tasks shall include: • The contractor shall perform DNA pronuclear microinjection of our BAC clone into the C57BL6 mouse strain (MOUSE #1 KI/KO PROJECT (BAC) ) • The contractor shall provide breeding, housing, care, and genotyping for this BAC mouse project • The contractor shall make a TARGATT vector using a TetO-oncogene that will be provided by our lab • The contractor shall generate AttB-TetO-Oncogene construct, and confirm the construct by enzyme digestion and sequencing, as well as purifying the construct and verifying its purity. (MOUSE #2 TARGATT PROJECT) • The contractor shall generate TARGATT integrase • The contractor shall do TARGATT DNA pronuclear microinjection into the C57BL6 mouse strain • The contractor shall provide animal housing, care, and genotyping during the course of this project. B. Reports : At the completion of each milestone, the contractor shall send a written report to the Brownell Laboratory. In addition, the contractor shall communicate weekly via email/phone to maintain continuity. For Mouse #1 KI/KO project (BAC) the report shall include Mouse generation steps (client is providing the targeting vector construct to ASC). For Mouse #2 TARGATT project, the reports shall include: • DNA construct design, schematic map, sequence and restriction enzyme digestion results; • Generation of Integrase mRNA, microinjection, embryo implantation and Quality Control; • Generation of transgenic mouse/mice and genotyping data This is not a solicitation for competitive quotations. However, if any interested party, especially a small business, believes they can meet the above requirement, they may submit a proposal. All information furnished must be in writing and must contain sufficient detail to allow the NCI to determine if it can meet the above unique specifications described herein. The proposal must be received at the NCI Office of Acquisitions on or before 11:00 AM EST on September 10, 2013. All responses and questions must be in writing and can be faxed (301) 402-4513 or emailed to Tiffany Kittrell, Contract Specialist at Tiffany.kittrell@nih.gov. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award, contractors must have valid registration and certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Applications (ORCA) through sam.gov. No collect calls will be accepted. Please reference solicitation number NCI-130119-TK on all correspondences.
- Web Link
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FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/FCRF/NCI-130119-TK/listing.html)
- Record
- SN03164994-W 20130829/130828000402-72253110ff231acc25dcb97340d25810 (fbodaily.com)
- Source
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