SOURCES SOUGHT
B -- Messenger Ribonucleic acid (mRNA) Expression Services
- Notice Date
- 6/27/2013
- Notice Type
- Sources Sought
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, Food and Drug Administration, Office of Acquisitions and Grants Services, 5630 Fishers Lane, Room 2129, Rockville, Maryland, 20857-0001
- ZIP Code
- 20857-0001
- Solicitation Number
- 1118902
- Archive Date
- 7/18/2013
- Point of Contact
- Sondea R Blair, Phone: 8705437469
- E-Mail Address
-
sondea.blair@fda.hhs.gov
(sondea.blair@fda.hhs.gov)
- Small Business Set-Aside
- N/A
- Description
- MARKET RESEARCH PURPOSES ONLY NOT A REQUEST FOR PROPOSAL OR SOLICITATION The U.S. Food and Drug Administration (FDA), is conducting market research to support the National Center for Toxicological Research's service requirement for the assessment of gene messenger ribonucleic acid (mRNA) expression changes in human cell lines. This sources sought notice is being issued to determine if small business manufacturers or small businesses capable of supplying a U.S. made product that satisfies our requirement of another small business manufacturer exist. The associated North American Industry Classification System (NAICS) Code is 541711- Research and Development in Biotechnology; Small Business Size Standard is 500 employees. Background: The FDA's, National Center for Toxicological research (NCTR) requires services for assessing gene messenger ribonucleic acid (mRNA) expression changes in human cell lines for comparison of the effects of tobacco on the cellular expression of the gene. Messenger Ribonucleic acid (mRNA) Expression Services The Government intends to provide a minimum of 1.25 ug of total RNA and projects providing a total of 166 samples within 6 months of contract the award date. Contractor Deliverables: -The contractor shall independently assess the quantity and quality of the RNA to ensure that it is acceptable for sequencing. -Preparation of libraries from the mRNA shall be able to run on Next-Generation (Next Gen) platform. -Sequencing of the libraries shall be accomplished using Illumina sequencing technology. -The parameters for the sequencing shall be paired end reads, 50 bases minimum per read, and a depth of coverage of at least 12 million read pairs per sample. -Contractor shall ensure alignment of the read data to the current reference human genome and transcriptome with transcript identifications assigned from the human Gene table as the definition for both gene and transcript level expression, modified so that transcript Identification Numbers (IDs) are guaranteed to be unique. -Contractor shall ensure a minimum of 85% unambiguous alignment to the transcriptome and genome -The following data analysis and output shall be provided on a portable hard drive of sufficient capacity to hold all the data (multiple hard drives may be used if the amount of data require it): a. Raw data file for each sample b. A table of the flow cell statistics outlining the quality and yield of the flow cell(s) where samples were clustered and sequenced c. Quality Control (QC) files regarding any presence of artifact/duplicate sequences, along with base distributions and qualities d. A tab-delimited text file with collapsed and normalized counts for each detected gene and isoform for each specimen e. An alignment file (BAM format) containing best alignments of each read to genome f. De-multiplexing of Illumina barcodes, if applicable g. Provision of a tab-delimited file containing normalized counts of each gene Qty: Minimum 166 Samples Period of Performance -9 months Upon receipt of the samples, the vendor shall complete the sequencing within 30 days and send the described deliverables within the following 60 days. Place of Performance Work shall be performed on-site at the contractor location with deliverables sent to the following Governmentlocation: US Food and Drug Administration National Center for Toxicological Research Jefferson Laboratory 3900 NCTR Road Jefferson, AR 72079 Responses to this sources sought shall unequivocally demonstrate that the respondent is regularly engaged in the manufacturing and/or sale of same or substantially similar items. Though the target audience is small business manufacturers or small businesses capable of supplying U.S. made product of another small business manufacturer or producer, all interested parties may respond. At a minimum, responses shall include the following: •Business name and bio, DUNS number, business address, business website, business size status (i.e., SB, VOSB, SDVOSB, HUBZone SB, SDB, WOSB, LB), point of contact name, mailing address (if different from business address), phone number and email address; [Provide this same information again is responding to provide a product manufactured by another firm]. •Past performance information for the manufacture and/or sale of same or substantially similar items to include date of sale, description (should also include drawings and photos), dollar value, client name, client address, client contact name, client point of contact mailing address (if different from that provided for client), client point of contact phone number, client point of contact email address, and name of the manufacturer (to include DUNS number and size status) if not the respondent. •Descriptive literature, brochures, marketing material, etc. detailing the nature of the items the responding firm is regularly engaged in manufacturing and/or selling. •If applicable, identification of the firm's GSA Schedule contract(s) by Schedule number and SINs that are applicable to this potential requirement are also requested. •If a large business, provide if subcontracting opportunities exist for small business concerns. The government is not responsible for locating or securing any information, not identified in the response. Interested Contractors must respond with capability statements which are due in person, by postal mail or email to the point of contact listed below on or before July 03, 2013 by 10:00 hours (Central Time in Jefferson, Arkansas) at the Food and Drug Administration, Field Operations Branch, FDA|OO|OFBA|OAGS|DAP, Attn: Sondea Blair, 3900 NCTR Road, HFT-320, Bldg 50, Rm 421 Jefferson, AR 72079-9502 or email sondea.blair@fda.hhs.gov. Reference FDA 1116032. Notice of Intent Responses to this sources sought announcement will assist the Government in determining whether or not any future requirement similar to this one should be set aside for small business, made available to full and open competition or procure through sole-source acquisition procedures. Disclaimer and Important Notes This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in FedBizOpps. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s). Additional Notes: If the stated requirements appear restrictive, please submit comments detailing the concern. Though this is not a request for quote, informational pricing for the service is encouraged.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/FDA/DCASC/1118902/listing.html)
- Place of Performance
- Address: Deliverables shall be sent to the following Government location:, US Food and Drug Administration, National Center for Toxicological Research, Jefferson Laboratory, 3900 NCTR Road, Jefferson, Arkansas, 72079, United States
- Zip Code: 72079
- Zip Code: 72079
- Record
- SN03102121-W 20130629/130628000800-694d47086c4379d71c4182633edad274 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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