SOLICITATION NOTICE
A -- Exome Sequencing Services
- Notice Date
- 12/21/2012
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-CSB-(HG)-2013-046-DLM
- Archive Date
- 1/15/2013
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- THIS IS A COMBINED SYNOPSIS/SOLICITATION FOR COMMERCIAL SERVICES PREPARED IN ACCORDANCE WITH THE FORMAT IN FAR SUBPART 12.6 AS SUPPLEMENTED WITH ADDITIONAL INFORMATION INCLUDED IN THIS NOTICE. THIS ANNOUNCEMENT CONSTITUTES THE ONLY SOLICITATION; PROPOSALS ARE BEING REQUESTED, AND A WRITTEN SOLICITATION DOCUMENT WILL NOT BE ISSUED. The solicitation number is NHLBI-CSB-(HG)-2013-046-DLM. The solicitation is being issued as a Request for Quotations (RFQ). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-62, December 20, 2012. The associated North American Industrial Classification System (NAICS) code for this procurement is 541711, Research and Development in Biotechnology with a business size standard of 500 Employees. This acquisition is being conducted in accordance with the procedures of Simplified Acquisition Procedures (Simplified Acquisition Threshold of $150,000.00) as set forth in FAR Part 13. Offerors must submit all questions concerning this solicitation in writing to dorothy.maxwell@nih.gov or maxwelld@mail.nih.gov. All questions must be received by 7:30 a.m. Eastern Time, December 31, 2012. All responses to questions will be made in writing, without identification of the questioner, and will be included in an amendment to the solicitation on or before, December 31, 2012. STATEMENT OF WORK AS FOLLOWS : Background : The National Human Genome Research Institute (NHGRI), Undiagnosed Disease Program (UDP) is a pilot program designed to address the needs of persons with debilitating medical conditions for which no diagnosis has been found despite an extensive workup. The goals of the UDP include finding accurate diagnoses and discovering new diseases that provide insight into human physiology and genetics. The UDP was established to evaluate participants who have undiagnosed medical conditions. UDP participants have generally received extensive diagnostic workups before traveling to the NIH and often have numerous and/or complex medical problems. The UDP program makes use of a diverse set of diagnostic and research techniques both to attempt to find a diagnosis and to generate research projects to advance medical knowledge. Purpose and Objectives : The purpose of this particular sequencing project is to perform clinical grade quality whole exome sequencing on patient and familial DNA. All data must be produced using the same instrumentation and methodology that the UDP has used thus far in order to allow integration of this data with it current databases and analysis using in-house software programs. Contractor Requirements: The contractor must provide the following : • Exome sequencing of 50 purified genomic DNA samples from the NIH Undiagnosed Diseases Program, without the need for phenol/chloroform extraction; • Exome sequencing must be performed using the Illumina HiSeq 2000 or 2500 and related instrumentation and software; • Enrichment must be performed using the Illumina TruSeq Enrichment Kit; • Samples must be sequenced to a least 75-fold (75X) average sequencing coverage at the raw data level with 100 bp paired-end sequencing method; • Turnaround time must be 8 weeks or less including sample QC; (NO Exceptions) • Raw data in the form of Fastq and BAM will be provided in a hard disk drive to the NIH UDP; • The contractor shall pay for shipment of the samples to the sequencing laboratory; • The contractor shall provide real time access to the status of the project, download reports and data immediately after they are available; • The contractor shall provide managerial support for the project 24/7. The sequencing instrumentation parameters, chemistry, and report data format must remain consistent with the results previously obtained in order to perform WES filtering and analyses with SNP data using programs developed in-house and to allow for direct comparison. Period and Place of Performance : • The contractor shall be provided 50 DNA samples for Exome sequencing by the Government. Clinical grade quality sequencing data will be provided on a hard disk drive to the NIH Undiagnosed Diseases Program in Fastq and BAM forms within 8 weeks of submission. NIH is located in Bethesda, Maryland. EVALUATION FACTORS: EVALUATION OF QUOTES : The Government intends to award a single contract from this solicitation. The Government reserves the right not to award a contract. Evaluation of Quotations: The Government reserves the right to make an award without discussions based solely upon initial proposals. EVALUATION FACTORS : (Total Factor Points: 100) 1. Sequencing must be performed on the Illumina HiSeq2000 or 2500 and enrichment performed using the Illumina TruSeq Enrichment Kit on genomic DNA purified without phenol/chloroform extraction.(30 Points) 2. Sequencing must be performed to a minimum 75X-fold average (higher is fine) coverage using 100 bp paired-end sequencing method. (30Points) 3. Raw data must be supplied as lane reads on a hard disk in Fastq format. (30 Points) 4. BAM files (10 Points). A. Price: (This will be Firm-Fixed Price): An evaluation of the offeror's price proposal ` will be made to determine if proposed prices are realistic for the work to be performed, reflect a clear understanding of the requirements, and are consistent with the technical proposal. Reasonableness determinations will be made by determining if competition exists, by comparing proposed prices with established commercial or GSA price schedules (if applicable), and/or by comparing proposed prices with the Independent Government Cost Estimate (IGCE). B. The Offeror must include in their quotation the price/unit price, labor rates, quantity, travel, shipping, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. In accordance with FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items, offerors must complete annual representations and certifications on-line at https://www.sam.gov/portal/public/SAM/. This contract incorporates one or more clauses by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. Also, the full text of a clause may be accessed electronically at this address: https://farsite.hill.af.mil/vffar1.htm CLAUSES INCORPORATED BY REFERENCE (FAR 52.252-2) (FEB 1998) 52.213-4: Terms and Conditions--Simplified Acquisitions (Other Than Commercial Items) Offerors shall submit their quotations no later than 7:30 a.m. Eastern Standard Time (EST) on December 31, 2012. The quotation proposals must reference the RFQ Number: NHLBI-CSB-(HG)-2013-046-DLM. All responsible offerors may submit a quotation proposal, which if timely received, shall be considered by the agency. Quotation Proposals can be emailed to the Contracting Specialist, Dorothy Maxwell at dorothy.maxwell@nih.gov or maxwelld@mail.nih.gov; Quotations shall not be deemed received by the Government until the quotation is entered into the e-mail address inbox set forth above. Faxed proposals will NOT be accepted. Offerors' proposals shall not be deemed received by the Government until the proposal is entered into the e-mail address inbox set forth above.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-(HG)-2013-046-DLM/listing.html)
- Place of Performance
- Address: NIH, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN02954752-W 20121223/121221234952-70ad0d5d2f5a166094007c80a7a99f70 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |