SOURCES SOUGHT
66 -- Bioreactor Culture System
- Notice Date
- 7/15/2011
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of the Air Force, Air Force Materiel Command, ASC/PKO, 1940 ALLBROOK DRIVE, WRIGHT-PATTERSON AFB, Ohio, 45433-5309, United States
- ZIP Code
- 45433-5309
- Solicitation Number
- FA8601-11-T-0202SS
- Archive Date
- 7/29/2011
- Point of Contact
- Michael J. Allen, Phone: 9375224586
- E-Mail Address
-
michael.allen2@wpafb.af.mil
(michael.allen2@wpafb.af.mil)
- Small Business Set-Aside
- N/A
- Description
- This is a sources sought announcement only, and is not an intent to procure. A solicitation is not available at this time, and any requests for a solicitation will not receive a response. ASC/PKOA Contracting at Wright Patterson AFB, OH, is conducting market research to determine the availability of firms to provide a bioreactor culture system that will allow the growth of multiple large-scale bacterial cultures simultaneously and will also allow for monitoring and manipulation of bacterial growth conditions. We require two bioreactor systems, each consisting of an independent controller and a 2L vessel. Two independent systems are required to duplicate experiments, as well as to examine the influence of variables between the two systems. We specifically require a 2L vessel since the system will be used to "feed" a secondary growth chamber (to be acquired via a different mechanism), whole volume is also approximately 1L. A larger vessel would waste reagent, and therefore waste funds; a smaller system would not allow adequate circulation/turnover in the secondary growth chamber. System should provide control of mixing speed; addition of a medium, acid, base, and gas; and heating and cooling. It should monitor parameters such as pH, dissolve oxygen, temperature, medium level and biomass. We require that the system control temperature of bacterial cultures (up to 70 degrees C) via a silicone heating blanket and cold finger, with cold water valve and recirculating water chiller. Gas flow of air and oxygen should be included and controlled by rotometers. Oxygen flow should additionally be controlled by a solenoid valve. System must also be capable of running in a BioSafety Level 2 facility, so the bioreactor should be autoclave after experiments are finished. Bioreactor should be compatible with use in a closed system to flow medium out of the bioreactor and into a secondary vessel. Below are a summary of the major technical requirements necessary to meet our needs are: - Vessel should have a 2L total volume. - Vessel should be autoclave. - Vessel should have air outlet with condenser to prevent evaporation of liquid medium. - Vessel should be single walled. - Vendor will provide recirculaing water chiller with by-pass return. - System should include heating blanket for upward temperature control. - System should include cold finger for downward temperature control. - System should have auto-tuning PID to provide accurate temperature, pH, and dissolved O2 control without over shooting or fluctuating around parameter set point. - System should include sensors for dissolved oxygen, pH, level and temperature. - System should include at least 3 liquid pumps for acid and base addition and level control. - System should be capable of dose monitoring, i.e. track total volume of liquids added. - System should include a noninvasive biomass monitor to track optical density with these tech specification: o Continuous, real-time measurements o Linear response range: 0.1-300 OD units o Able to integrate with controlled by fermentor system o Baseline setting and subtraction o Operating range of 0 to 50 degrees C o Water resistant/splash proof - System should have I/O expansion capabilities with no fewer than: o 4 x differential or 8 single ended analog inputs o 4 analog outputs o 8 digital inputs o 8 digital outputs For the integration of additional accessories (pumps, sensors, temperature controllers, etc.) - System shall be agitated by motor-driven Rushton impeller and include a spin filter impeller (10um) - System should include 2 rotometers for air and oxygen flow. - Oxygen flow should be controlled by solenoid valve - System should include data acquisition software that allows monitoring and analysis of date generated during bacterial culture. - Head plate should have enough pors to accommodate: o Stirrer assembly o pH, dissolved oxygen, temperature and level probes/sensors o 6 dip tubes for acid, base, and media addition, removing spent media, circulating media o Sampling system (to be included) - System should come with liquid addition bottle and autoclavable rack Each potential source shall give a brief description of their product and ability to satisfy the requirements identified above. All responses should include product model number, manufacturer, specifications, delivery timeline, and any applicable warranties. Each potential source, if not the manufacturer, must be an authorized supplier capable of providing and honoring the commercial manufacturer's warranties on the prospective items that meet these requirements. All responses to this sources sought are limited to five pages and must include the following additional information: name and address of firm, commercial activity/government entity (CAGE) code, small or large business size status, point of contact name, title, phone, and email. Interested sources shall submit all replies by email to michael.allen2@wpafb.af.mil no later than 8:00 AM EST on Tuesday, 26 July 2011. Acknowledgement of receipt will not be made. THE GOVERNMENT DOES NOT INTEND TO AWARD A CONTRACT ON THE BASIS OF THIS SOURCES SOUGHT OR TO OTHERWISE PAY FOR THE INFORMATION SOLICITED. This request for information is for planning purposes only and shall not be considered as an Invitation for Bid (IFB), Request for Quotation (RFQ), or Request for Proposal (RFP), or as an obligation on the part of the Government to acquire any products or services. Your responses to this sources sought will be treated as information only. No entitlement to payment of direct or indirect costs or charges by the Government will arise as a result of contractor submission of responses to this sources sought or Government use of such information. The Government reserves the right to reject, in whole or part, any contractor input resulting from this sources sought. The information resulting from this sources sought may be included in one or more RFP's, which may be released via the FEDBIZOPPS.GOV portal.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USAF/AFMC/88 CONS/FA8601-11-T-0202SS/listing.html)
- Place of Performance
- Address: Wright Patterson AFB, Wright Patterson AFB, Ohio, 45433, United States
- Zip Code: 45433
- Zip Code: 45433
- Record
- SN02500762-W 20110717/110715235629-f020b3e0fd7be5ceaf4168f15841e019 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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