SOURCES SOUGHT
Q -- Deep Sequencing and Microarray Core
- Notice Date
- 7/5/2011
- Notice Type
- Sources Sought
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-CSB-(HL)-2011-233-DDC
- Archive Date
- 8/4/2011
- Point of Contact
- Deborah - Coulter, Phone: (301) 435-0368
- E-Mail Address
-
dc143b@nih.gov
(dc143b@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a Sources Sought Notice (SS). This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of ALL qualified sources that are interested in, and capable of, providing the Whole Exome Sequencing of Genes Underlying Subclinical Atherosclerosis services. The National Heart, Lung, and Blood Institute (NHLBI) does not intend to award a contract on the basis of responses nor otherwise pay for the preparation of any information submitted. As a result of this Sources Sought notice, the NHLBI may issue a Request for Quote (RFQ). THERE IS NO SOLICITATION AVAILABLE AT THIS TIME. However, should such a requirement materialize, no basis for claims against NHLBI shall arise as a result of a response to this Sources Sought notice or the NHLBI's use of such information as either part of our evaluation process or in developing specifications for any subsequent requirement. The NHLBI is seeking capability statements from all eligible business' under the North American Industry Classification System (NAICS) code 541712 with a size standard of 500 employees. All eligible business' responding to this Sources Sought Announcement must have the capabilities to provide the below services upon receipt of order. Interested parties are expected to review this notice to familiarize yourself with the requirements of this project; failure to do so will be at your firm's own risk. Background The National Institutes of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI), Cardiovascular and Pulmonary Branch, developed cell lines from patients with Lymphangioleiomyomatosis (LAM), a devastating lung disease of women. The cell lines developed are being used to understand the pathogenesis of this disease. A critical aspect for the understanding of the disease is to obtain information about the cells gene expression when treated with estrogen. We are also interested on determining the role of mutations in the tumor suppressor gene (Tuberous Sclerosis Complex 2) because it affects development of Lymphangioleiomyomatosis (LAM) which is a disease studied in this laboratory. This aspect is important because of the prevalence of LAM in women. We have studied many candidate genes involved in LAM but we can not get a full understanding of the disease. Thus, we would prefer to obtain a more global idea of the gene expression changes of these cells. Therefore, we would need to carry out gene expression profiles of cells non-treated or treated with estrogen. These experiments are essential for the goals of the branch. Purpose and Objectives: The main of objective of this requirement is to carry out gene expression profiles that would allow us to obtain data that will tell us differences of the genes present in each sample when treated with estrogens. The work will involve following services on the below samples: -Isolation of RNA from cells cultured in vitro. -Labeling of the RNA -Analysis of the molecules 1. Processing 40 Human samples on Illumina HT-12 beads arrays 2. Processing 48 18 mouse samples on Illumina WG-6 beads arrays 3. Processing 48 human samples on Illumina HT-12 beads arrays 4. Processing 24 mouse samples on Illumina WG-6 beads arrays The Government's estimated delivery time is 6 weeks from the time of sample shipment. Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s). All capability Statements sent in response to this SOURCES SOUGHT notice must be submitted electronically (via email) to Deborah Coulter, Contract Specialist, at coulterd@nhlbi.nih.gov in either MS Word or Adobe Portable Document Format (PDF), by July 20, 2011, 8:30 AM, EST. All responses must be received by the specified due date and time in order to be considered.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-(HL)-2011-233-DDC/listing.html)
- Place of Performance
- Address: John Hopkins Medical Institute, Baltimore, Maryland, 21205, United States
- Zip Code: 21205
- Zip Code: 21205
- Record
- SN02489589-W 20110707/110705234418-2de1914434d78baf6c67152a746780b0 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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