SOLICITATION NOTICE
65 -- cAMP HiRange Assay Kits
- Notice Date
- 3/17/2011
- Notice Type
- Presolicitation
- NAICS
- 339999
— All Other Miscellaneous Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-CSB-(HG)-2011-135-DLM
- Archive Date
- 4/6/2011
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- Total Small Business
- Description
- THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) intends to negotiate and award a purchase order on a noncompetitive sole source basis to CisBio US, Inc., 135 South Road, Bedford, Massachusetts 01730-2204 to procure cAMP HiRange Kits. The sole source determination is based on the fact that National Institute of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency conducting and supporting making medical discoveries that improve people’s health and save lives. The mission of the NCGC is to apply the tools of small molecule screening and discovery to the development of chemical probe research tools, for use in the study of protein and cell functions, and biological processes relevant to physiology and disease. NCGC will optimize biochemical, cellular and model organism-based assays submitted by the biomedical research community; perform automated high-throughput screening (HTS); and perform chemistry optimization on confirmed hits to produce chemical probes for dissemination to the research community. These reagents allow the detection of cAMP in a homogenous assay format, which is critical to high throughput screening (HTS). The quantities being purchased here are urgently requested and will be used for large collection robotic screen. This requested cAMP HiRange kit is designed to address either the use of cell-based or cellular membranes-assays in GPCR activation and Adenylate Cyclase activity screening. The cAMP HiRange assay is highly robust and provides quality results while eliminating the costly need for a continuous supply of living cells. HTRF® (homogeneous time resolved fluorescence) is a technology based on TR-FRET, a combination of FRET chemistry and the use of fluorophores with long emission half-lives. While HTRF is based on TR-FRET chemistry it has many properties that separate it from other TR-FRET products. These include the use of Europium and Terbium cryptate chemistries. The lanthanides have a long life and their complexing to macrocycles to form cryptates allows increased stability. Finally, the use of a patented radiometric measurement enables correction for quenching and sample interferences. This requested kit is one of three ready-to-use assays manufactured by CisBio for measuring cAMP using HTRF® technology. Each kit is optimized for detecting a specific range of cAMP concentrations that allows us the ability to fine tuning our assay sensitivity. These kits enable high quality, cell-based assay screening in 96-, 384- or 1536-well formats. The primary assay was developed and optimized using this reagent for the Gnas1 inhibitor project at the NCGC, and has been miniaturized into the 1536-well plate format for high throughput screening. A requirement of this assay is that the detection reagents are highly specific to cAMP and can detect the presence of cAMP in a homogenous format. We plan to use this assay in a high throughput screen aimed at finding small molecule compounds that will inhibit activating mutations of the Gnas1 protein. This project is an effort to develop drugs that might attenuate the Gs signaling pathway in treatment of McCune Albright Syndrome. To this extent, we will measure the intracellular level of cAMP in a cell line that carries the Gnas1 activating mutation. We must use the same variables in our assays to achieve the desired outcomes and to maintain the integrity and compatibility of the project studies. All of the earlier assay optimization done by our group was performed with the cAMP HighRange kits. Additionally, the screening needs to be done with the same reagents in order to yield comparable and readily interpretable results. A switch to a different detection reagent would necessitate re-optimizing the assay. This would entail unacceptable substantial time delays and enormous additional costs. This is a small business set-aside. Industry Classification (NAICS) Code is 339999, All Other Miscellaneous Manufacturing, with size standard of 500 Employees. The acquisition is being conducted under FAR Part 13, simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001).and the resultant purchase order will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-48 (January 31, 2011). This notice of intent is not a request for competitive proposals. Interested parties may identify their interest and capabilities in response to this synopsis, by March 22, 2011, 7:30 a.m. Eastern Standard Time. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurement. Inquires to this announcement, referencing synopsis number NHLBI-CSB-(HG)-2011-135-DLM, may be submitted to the National Heart, Lung and Blood Institute, Office of Acquisition, COAC Services Branch, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-(HG)-2011-135-DLM/listing.html)
- Place of Performance
- Address: NIH, Rockville, Maryland, 20850, United States
- Zip Code: 20850
- Zip Code: 20850
- Record
- SN02403729-W 20110319/110317235006-be67e18a0ae04bef0996cd42a71a159b (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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