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FBO DAILY ISSUE OF SEPTEMBER 04, 2010 FBO #3206
SOLICITATION NOTICE

A -- Gene Targeting Services

Notice Date
9/2/2010
 
Notice Type
Presolicitation
 
NAICS
541711 — Research and Development in Biotechnology
 
Contracting Office
Department of Veterans Affairs;VISN 22 Network Contracting Activity;Building 149;5901 E. 7th Street;Long Beach CA 90822
 
ZIP Code
90822
 
Solicitation Number
VA26210RQ0484
 
Response Due
9/17/2010
 
Archive Date
11/16/2010
 
Point of Contact
Jan Smith
 
E-Mail Address
SS<br
 
Small Business Set-Aside
N/A
 
Description
The VISN 22 Network Contracting Activity intends to negotiate a firm, fixed-price sole source procurement (Commercial Item FAR Part 12) with inGenious Targeting Laboratory to provide gene targeting services for the Research Department of the VA San Diego Healthcare System, 3350 La Jolla Village Drive, San Diego, CA 92161 under the authority of FAR 6.302-1. Services to include: (1) Cloning & Construction of Targeting Vector for Hybrid 129S6/SvEvTac/C57BL/6 cell line, including analysis of gene structure, screening strategy design, probe design, isolation and confirmation of genomic BAC clone, targeting vector construction including insertion and confirmation of homology arms, flanking of the genomic targeting region by Lox-P sites for Cre-excision, insertion and confirmation of Neomycin selection cassette flanked by FRT sites for positive selection, and preparation of DNA for electroporation; (2) Electroporation of Hybrid 129S6/SvEvTac/C57BL/6 ES cells with targeting vector and Tissue Culture Work to isolate antibiotic-resistant clones, and: (3) Screening of ES Cell Clones to identify positives, including preparation of DNA from individual ES clones and confirmation of targeting events by PCR and Southern Blotting. The VA believes that inGenious Targeting Laboratory is the only company that can provide the services required as the inGenious Targeting Laboratory developed the Hybrid ES cell technology, creating a new line (129S6/SvEvTac strain with the C57BL/6 line) and is the only company to offer electroporation into this line. This notice of intent is not a request for competitive quotes. However, any firm that believes it can meet these requirements may give written notification to the Contracting Officer no later than September 9, 2010 at 12:00PM. Supporting evidence must be in sufficient detail to demonstrate the ability to comply with the above requirements for services. A determination by the Government not to compete this proposed contract based on responses to this notice is solely by the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. All inquiries for this procurement must be sent, in writing, to the Contracting Officer via e-mail at Jan.Smith1@va.gov or faxed to (562) 961-1396. The requester must include the RFQ Number, requester's name, address, telephone number, fax number, and e-mail address.
 
Web Link
FBO.gov Permalink
(https://www.fbo.gov/spg/VA/LBVANBC/VAMD/VA26210RQ0484/listing.html)
 
Place of Performance
Address: VA San Diego Healthcare System;Research (664/09/151);3350 La Jolla Village Drive;San Diego
Zip Code: 92161
 
Record
SN02265720-W 20100904/100902235709-86dbc8603ce9dfca8517a6d12820fe9c (fbodaily.com)
 
Source
FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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