Loren Data's SAM Daily™

FBO DAILY ISSUE OF JUNE 27, 2010 FBO #3137
SOURCES SOUGHT

66 -- Computerized Stereology Investigation System

Notice Date

6/25/2010
 

Notice Type

Sources Sought
 

NAICS

511210 — Software Publishers
 

Contracting Office

Department of Health and Human Services, Food and Drug Administration, Office of Acquisitions and Grants Services, NCTR-50 RM422 HFT-320, Jefferson, Arkansas, 72079, United States
 

ZIP Code

72079
 

Solicitation Number

1075444
 

Archive Date

7/27/2010
 

Point of Contact

Nicholas E Sartain, Phone: 870-543-7370
 

E-Mail Address

nick.sartain@fda.hhs.gov
(nick.sartain@fda.hhs.gov)
 

Small Business Set-Aside

N/A
 

Description

THIS IS FOR MARKET RESEARCH PURPOSES ONLY, NOT A REQUEST FOR PROPOSAL OR SOLICITATION. The Food and Drug Administration, Jefferson Laboratories, in Jefferson, Arkansas, is seeking small business sources, including, service-disabled veteran-owned small businesses, HUBZone small businesses, woman-owned small business and 8(a) certified companies. The government would also like large businesses capable of meeting this requirement to respond as well. The following information is provided to assist the FDA in conducting Market Research to identify potential contractors for this effort. The government is seeking sources capable of providing a computerized stereology investigation system plus apparatus for performing brain mapping, neuron tracing, anatomical mapping, and morphometry. The system to include stereo investigator microscope, computer and software/hardware. The government is requesting information from potential contractors that have a system similar to that of MBF Bioscience Neurolucide and Stereo Investigator microscope. The system should be capable of performing design-based stereology on isotropic or vertical (anisotropic) sections. The vendor must provide the installation, training and warranty. The system will be used to support studies involving research into sexual hormone-like compounds extensively exist in nature foods and/or food-processing chains and render physiology-interfering or pathophysiological impacts on human central nervous system, noticeably the sexual dimorphic brain structures. The current method in evaluation of the compounds in neurotoxicity, determination of changes in size of the sexual dimorphic brain structures of the experimental animals, is single-disciplinary approaching (anatomic or developmental neurobiological) and thus less sensitive, indicating the unmet need for general public safety screening of sexual hormone-like compounds. Our objective is to establish a multidisciplinary approach in assessing those compounds. We will investigate the brain structures by conventional histology, immunohistochemistry and projection-tracing marker(s), which simultaneously addresses morphological and biochemical bases and functional connection as well. Stereology is the state of the art technology that provides quantitative measurements necessary for the proposed tasks. MBF system components are as follows: A NL-15 Neurolucida - microscope based scientific research software 1)-3D Neuron Tracing and Reconstruction • -3D Serial Section Reconstruction • -Brain Mapping and Anatomical Mapping • -Quantitative Morphometric Analysis • -Image Analysis • -Particle Counting • -Create high resolution, publication quality 3D models • -Direct export of results to Microsoft Excel 2) SS-15 Stereo Investigator - advanced software for Design Based Stereology • -Automated workflows for Optical Fractionator • -Automated workflows to acquire multi-channel fluorescent 3D image stacks with systematic random sampling for offline analysis • -Automatic Contour Tracing • -Serial Section Manager • -2D Anatomical Mapping • -Automatic exportation of data and results to Microsoft Excel 3) NS-21 Image Stack Extension Module • -Capacity to analyze confocal brightfield and widefield fluorescence 3D image stacks. Require the capability to create and export brightfield and widefield fluorescence image stacks to Neurolucida and Stereo Investigator offline workstations. Require 3D image visualization. Requires Solid Modeling module for 3D surface rendering of traced neurons or ROI S. 4) NS-31 Solid Modeling Extension Module • -Serial section reconstructions appear as solid objects that can be dynamically rotated to any angle, and resized to any magnification. • -Real-time voxel rendering of 3D images • -Surface transparency and lighting conditions • -3D visualization of models (rotate and zoom) • -Easily export 3D model in large-format for publications and posters 5) NS-22 Virtual Slice Extension Module • -Capability of creating extremely high-resolution montages composed of images obtained from multiple microscopic fields-of-view. • -Require the resolution of the individual fields maintained in the montage, allowing for the capacity to zoom in and out without loss of resolution. Combine high magnification detail resolution of large fields of view provided by lower power objectives. 6) MS-S5 Software integration module • -for controlling automated microscope functions of a Zeiss AxioImager M2 and Z2 upright microscope model. 7) MS-S6 Software integration module for controlling motorized stage. 8) SD-60 Special motorized stage system • -motorized stage system: motorized stage, controller, encoder and software drivers along with brightfield software. 9) NL-87 Apotome Software Integration Module. Required for Neurolucida or Stereo Investigator software. 10) NL-11 Neurolucida - Workstation version • (no control of motorized stage or frame grabber) For Mapping, Neuron Tracing, 3D Serial Section Reconstruction, Morphometry, and Image Analysis. Includes the capability to import and analyze confocal, brightfield and widefield fluorescence 3D image stacks. • Includes 3D Volume Visualization enhancement software. • Requires Windows 7 or XP operating system. 11) NL-27 Upgrade Neurolucida/Workstation license (with attached modules) to a mobile license. • -Allows installation of multiple copies of Neurolucida, only one of which can be used at any given time. 12) NL-17 AutoNeuron upgrade for Neurolucida - Workstation version. • -Significantly enhances productivity for neuron tracing. This module provides fully automatic or inter-active neuron reconstruction from 2D images and 3D image stacks. Require/ include AutoNeuron workflow to guide and direct user through each step of the automated tracing process. Also add capability to produce 3D solid models of 3D serial section and 3D neuronal reconstructions. 13) NM-09 Neurolucida 3-Year+ Software Upgrade and Support Subscription. • -For new systems or renewals less than 2 years old. Provides 42 months of service for the cost of 36. B Computer Hardware/ or incidental PC components______ PC-90a PC workstation. • -2 units, one for Neurolucida (offline) PC, one for microscope, cameras, stage workstation PC. • -Intel® Quad Core Xeon Processor W3540 2.93GHz, 8MB L3 cache, 12GB DDR3 SDRAM, 2x500GB HD's in RAID 1 configuration. 1GB nVidia 9800 GT video card (dual DVI and/or VGA), 16X DVD+RW/+R drive, Enhanced Multimedia Keyboard, 5-button laser mouse, integrated Gigabit NIC. Windows 7, MUST be 64-bit operating system. • -Technical Support of at least 3 years. This is a custom designed PC which MUST be configured to the proprietary software necessary above and herein. C_____________Custom Imaging/camera components____________ 1) DV-50ª Monochrome digital camera. 2/3" chip • -1344H x1024V picture elements native output at 8+ frames per second. • - 12-bit, 1.37 megapixel imaging. Optimized for far-red fluorophores/epifluorescent imaging. Requires Firewire PC interface card and custom camera interface software for 32-bit and 64-bit systems. Requires a 426113 C-Mount Adapter - 60N C 0.63x, on the microscope. 2) DV-47d Color camera. • -1600H x 1200V picture elements native output at 10 frames per second. • -Multiple alternative binning modes (up to 8x8) frame rates, 1.92 megapixel imaging. Wide-bandwidth light sensitivity, optimal for brightfield and epifluorescent applications. Require software interface for 32-bit and 64-bit systems and Firewire PC interface card. Requires a ZCM-10a adjustable C-Mount Adapter - 60N to C-Mount, 1.0x adaptor on the microscope. D.___________Fluorescent and brightfield Microscope and interface components______ LM-330a Zeiss AxioImager M2 microscope system • configured for brightfield and multi-channel fluorescent work with Neurolucida and/or Stereo Investigator. Includes LED lamp, X-Cite120Q w/Iris fluorescent light source with spare bulb, motorized fluorescent filter turret and shutter, manual encoded 7x objective turret,manual condenser and dust cover. Objectives, C-Mounts and Filter Cubes separate. - ZO-20 Objective Set: • 2.5x, 5x, 10x, 20x, 40x Plan NeoFluar and 100x/1.40 Oil Plan Apo objectives -) ZDP-20 Motorized dual photo port upgrade kit for AxioImager -) ZAP-10 Zeiss Apotome with 3 Grids (HI, MI, LI) and cable -) FCA-302 Fluorescent filter cube set for DAPI (or Alexa 350, AMCA, BFP, Hoechst, etc.) Shift free -) FCA-338a Fluorescent filter cube set for Cy2 (or Alexa 488, FITC, eGFP, etc.) - Shift free -) FCA-343a Fluorescent filter cube set for Cy3 (or CFP, Alexa 546, 555, DiI, DsRed, Rhodamine, TRITC, etc.) shift free -) FCA-350 Fluorescent filter cube set for Cy5 (or Alexa 647, etc.) - Shift free $1,222 - ) VP-10 Vibration isolation platform for microscope. • Broad band vibration filter, especially efficient at low frequencies. Isolation bearing configuration will vary according to selected model of microscope __Tools and Hardware for incidental apparatus, instrument components__ A) CS-10 Calibration grid slide with reference grid squares in dimensions of 25um and 250um increments B) TK-10 Standard MBF Toolkit: • Extra long metric ball hex wrenches (1.5, 2.0, 2.5, 3, 4, 5mm). (1) English ball hex wrenches (5/64" to 5/16"). (1) Screwdriver set (slotted 1, 2, 3, 4mm, hillips #000, #00, #0, #1). C) ME-10 Focus Measurement Encoder • -Provides accuracy to 0.1um of the actual microscope stage focus position, 12mm range. Achieve closed-loop focus operation for more accurate Z-axis measurements via included microscope mounting bracket. 4. MS-88 2-axis computer-controlled stepping motor system • -comprised of 4"x3" XY stepping stage with linear encoders (0.05 um resolution, <3um accuracy), joystick, slide holder and 3-axis controller (Z axis for encoder feedback) with interface to PC. E) 426113 C-Mount Adapter • -60N C 0.63x for monochrome camera. F) ZCM-10a Adjustable C-Mount Adapter • -60N to C-Mount, 1.0x for color camera. __Training and set up for apparatus, instrument components__ A) OS-34 Three days of onsite installation/training. • Installation and training details to be determined in conjunction with trip scheduling. B) SI-21b MBF Zeiss ApoTome system integration fee. • Integration and testing of ApoTome systems supplied by vendor at vendor offices C) SI-10a System integration fee. • System testing/QA activites performed at vendor Bioscience, prior to system delivery. 4. IR-65 Remote training via telephone and VNC, 8 hrs @ $/hour The associated North American Industry Classification System (NAICS) Code is- 511210- Software Publishers; Small Business Size Standard is $25.0 Million. Potential contractors must indicate business size, DUNS number and proof of any set-aside certification and provide a contact name, the mailing address, phone number and email address of point of contact. Capability statements must provide enough information to determine if the company can meet the requirement. All descriptive material necessary for the government to determine whether the product offered meets the technical requirements including: technical specifications, descriptive material, literature, brochures and other information, which demonstrates the capabilities of the contractor to meet the requirement. The potential contractor should provide contact information to users of their proposed system and an estimated price range ($50k-$100k, $100k-$200k, etc). Interested Contractors must respond with capability statements which are due in person, by postal mail, fax or email to the point of contact listed below on or before July 12, 2010 by 13:00 hours (Central Time in Jefferson, Arkansas) at the Food and Drug Administration, OC/OA/OAGS, Attn: Nick Sartain, 3900 NCTR Road, HFT-320, Jefferson, AR 72079-9502. Reference FDA1075444. For information regarding this market survey, please contact Nick Sartain at (870) 543-7370, FAX (870) 543-7990 or email nick.sartain@fda.hhs.gov. Disclaimer and Important Notes. This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)."
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/FDA/NCTR/1075444/listing.html)
 

Place of Performance

Address: 3900 NCTR Road, Jefferson, Arkansas, 72079, United States

Zip Code: 72079
 

Record

SN02188373-W 20100627/100625234703-9df984c5db74100d4f52fcd8c53a58db (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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