SOLICITATION NOTICE
65 -- SpectraMax M5e Multimode Plate Reader
- Notice Date
- 10/20/2009
- Notice Type
- Presolicitation
- NAICS
- 423450
— Medical, Dental, and Hospital Equipment and Supplies Merchant Wholesalers
- Contracting Office
- US Army Medical Research Acquisition Activity, ATTN: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014
- ZIP Code
- 21702-5014
- Solicitation Number
- W81XWH10T0020
- Response Due
- 10/23/2009
- Archive Date
- 12/22/2009
- Point of Contact
- Suzanne Rogers, 301-619-1020
- E-Mail Address
-
US Army Medical Research Acquisition Activity
(suzanne.rogers@us.army.mil)
- Small Business Set-Aside
- N/A
- Description
- THIS NOTICE IS PROVIDED FOR INFORMATION PURPOSES ONLY. THIS OPPORTUNITY IS AVAILABLE ONLY TO CONTRACTORS UNDER W81XWH10T0020. SpectraMax M5e microplate reader must have the following performance specifications: Must have a 3 mode (Abs, FI, Lum) cuvette port and 6- to 384- well microplate capabilities for all 5 modes (ABS, FI, TRF, FP, Lum). Must be certified for Cisbio Internationals HTRF assay. Must read absorbance in standard, commercially-available cuvettes and microcuvettes, 12 x 75 mm test tubes, and 96- or 384-microplates with a built-in cuvette port and a microplate drawer in the reader and performed with a single wavelength selection. Must use a Xenon flash lamp for longer lamp lifetime. Must have Dual Monochromators for tunable FP wavelength selection between 400 nm and 750 nm for excitation and emission wavelengths. Must have Dual Monochromators for tunable FI, TRF wavelength selection between 250 nm and 850 nm for excitation and emission wavelengths and tunable luminescence between 250 nm and 850 nm. Must have tunable absorbance wavelength selection between 200 nm and 1000 nm. No filters are required for purchase in the future when additional assays are added to laboratory. Must have both top and bottom reading optics for improved sensitivity in cell-based assays such as chemotaxis membrane migration assays. Must have a 4 nm absorbance bandwidth for superior peak resolution and increased accuracy of measurements for narrow bandwidth samples. Must have fixed fluorescence spectral bandwidth of 9 nm excitation and 15 nm emission for improved resolution between EX/EM components. Narrow bandwidth is critical when designing multi-fluorophore assays or when using narrow Stokes shift fluorophores. Must read up to four EX/EM wavelength pairs per microplate for multi-fluorophore assay design. Must have full wavelength scanning capability across the entire spectral range for defining actual EX and EM maxima. Can perform spectral scans in user-defined increments as small as 1 nm, and then can select assay wavelengths for actual maxima. Increases sensitivity for assay by working at actual conditions rather than using literature references. Must have Automatic PMT setting to adjust the optimum voltage for each well sample concentration and then normalizes the raw data to show RFU values. Wells are selectively reread at the optimal PMT setting. Photo-bleaching is eliminated because wells are not read unnecessarily. Must have dual PMTs: an enhanced, low background PMT for high sensitivity luminescence measurements including luciferase-based reporter gene assays and a separate digital/analog PMT for fluorescence reads. Must have the option to run the reader with a slow carriage speed to increase assay precision. Must have the following read times (minutes:seconds): 96-well 384-well " Absorbance 0:18 0:49 " Fluorescence Intensity 0:17 0:48 " Fluorescence Polarization 0:42 2:03 " Time-Resolved Fluorescence 0:17 0:48 " Luminescence 2:00 7:00 Must have thermal regulation for temperature dependent kinetic assays. Temperature is regulated up to 60C. Three heat sources provide uniform temperature regulation across the plate (+/- 0.5C well-to well @ 37C). One heat source above the plate creates a small temperature differential eliminating plate lid fogging during kinetic runs. Must offer compliance support tools including: " Software validation package " IQ/OQ/PQ documentation " Physical validation test plates for absorbance and fluorescence Must offer automatic validation of absorbance performance with the SpectraTest" ABS1 validation plate. The SpectraTest ABS1 validation plate provides NIST traceability, and performs 8 tests to easily verify optical performance for GXP compliance. Must offer automatic validation of fluorescence performance with the SpectraTest" FL1 validation plate. The SpectraTest FL1 validation plate performs 12 tests to easily verify optical performance for GXP compliance. Must be robot-compatible for automation without requiring any instrument modification. Must ship with software that provides microplate reader data acquisition and analysis support for both Windows XP and Macintosh OS X operating systems. Software must offer a common file format that allows data files to be edited, saved, and shared by users on both Windows XP and Macintosh OS X, provided on one CD-ROM. Software must ship with many read-to-run protocols for a variety of common assay applications. Software must have ability to design custom assay protocols or customize existing protocol to create meaningful reports, graphs, and multi-plate summaries, including data trend analysis. Also must be able to save protocol as a menu-available choice so that one touch of the Read button will allow completion of data report in final format. Software must allow for the ability to auto save multiple copies of assay data in all desired formats: " SoftMax Pro native ".TXT files ".XML files ".PDF Files (Mac OS X) Software must allow the following data analysis tools: " Simultaneous evaluation of multiple standard curves " Ability to easily graph multiple plots on multiple graph sections " 4- and 5-parameter curve fitting with parallel line analysis " BOOLEAN operators If, And, Or, Not, False, True " Auto-repair of broken formula links Software must support regulatory compliance requirements such as FDA 21CFR part 11, GLP, GMP, etc (collectively referred to as GXP). Software must have control over virtually every aspect of the microplate reader solution to meet these GXP regulations. These controls include required user logon, management of guest access, granular permission structure, support for electronic signatures, operation of reader within permissions structure, complete experiment audit trails, network and local AutoSave.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USA/USAMRAA/DAMD17/W81XWH10T0020/listing.html)
- Place of Performance
- Address: US Army Medical Research Acquisition Activity ATTN: MCMR-AAA, 820 Chandler Street Frederick MD
- Zip Code: 21702-5014
- Zip Code: 21702-5014
- Record
- SN01989021-W 20091022/091020235846-1ab0d8e9227fa8b60e8a355e735296a2 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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